Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A stable serum pepsinogen II assay kit and its preparation method and application

A technology of pepsinogen and kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of susceptibility to chylomicrons (CM), inconvenient storage of reagents, expensive instruments and equipment, etc., and achieve enhanced anti-interference ability , excellent performance indicators, excellent stability

Active Publication Date: 2021-05-18
中拓医学检验有限公司 +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Double-antibody sandwich immunochemiluminescence assay (ILMA) has high accuracy and sensitivity, but the equipment is expensive, reagents are inconvenient to store, and the price is high; colloidal gold colorimetry is simple to operate and produces fast results, but it can only be used for qualitative research and cannot Quantitative
Latex immunoturbidimetric method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. ) impact and other shortcomings, the present invention improves on the shortcomings of the existing pepsinogen II kit to meet the requirements of clinical testing and chemical analysis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A stable serum pepsinogen II assay kit and its preparation method and application
  • A stable serum pepsinogen II assay kit and its preparation method and application
  • A stable serum pepsinogen II assay kit and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A conventional pepsinogen II assay kit, which includes reagent R1 and reagent R2.

[0052] Reagent R1 contains the following components:

[0053] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0054] NaCl 9g / L;

[0055] Sodium hydroxide 0.1g / L;

[0056] Propylene glycol fatty acid ester 1%;

[0057] Polyoxyethylene Sorbitan Hexa Stearate 1%;

[0058]proclin300 1 ml / L.

[0059] Reagent R1 has a pH value of 7.2.

[0060] Reagent R2 contains the following ingredients:

[0061] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0062] Mouse anti-human PGII antibody coated latex particles 30mg / L;

[0063] Bovine serum albumin (BSA) 5g / L;

[0064] Polyvinylpyrrolidone k30 5g / L;

[0065] Mannitol 5g / L;

[0066] Glycerol 10ml / L;

[0067] Triton 100 1%;

[0068] proclin300 1ml / L.

[0069] Reagent R2 has a pH of 6.8.

[0070] Wherein said percentage is volume ratio.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a pepsinogen II (PGII) assay kit, the kit contains reagent R1 and reagent R2; reagent R1 contains the following components: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, NaCl , sodium hydroxide, surfactants, preservatives. Reagent R2: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, mouse anti-human PGII antibody coated latex particles, suspending agent, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit can effectively avoid the interference of chylomicrons, and adopts different kinds of protective agents and suspending agents at the same time. It is a kind of kit with strong stability, high sensitivity and good repeatability. Low cost liquid kit.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent assay, in particular to a pepsinogen II assay kit, and also to a preparation method and application of the pepsinogen II assay kit. Background technique [0002] Pepsinogen (pepsinogen), synthesized by the main cells of the oxyntic gland, becomes pepsin in the gastric cavity through the action of hydrochloric acid (HCL) or active pepsin (pepsin), and decomposes protein into fat, peptone and a small amount of peptides . The optimum pH for the enzyme action is 2, and the enzyme activity is lost after entering the small intestine. [0003] Pepsinogen is the precursor of pepsin. According to its biochemical properties and immunogenicity, it is divided into 2 subgroups. The immunogenicity of components 1-5 is the same, called pepsinogen I, which is mainly composed of fundic glands. Secreted by chief cells and mucus neck cells; components 6 and 7 are called pepsinogen II, in addition to be...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/573G01N33/543G01N33/539
CPCG01N33/539G01N33/54313G01N33/573
Inventor 刘安娜
Owner 中拓医学检验有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com