An angiotensin-converting enzyme assay kit and its preparation method and application

An angiotensin and converting enzyme technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of poor repeatability, poor anti-interference performance, etc., to ensure specificity, promote catalysis, Uniform and stable reaction system

Active Publication Date: 2021-03-30
中科梅奥(山东)医学检验有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the activity of angiotensin-converting enzyme is easily inhibited by factors such as bilirubin, common angiotensin-converting enzyme detection reagents have disadvantages such as poor repeatability and poor anti-interference performance.

Method used

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  • An angiotensin-converting enzyme assay kit and its preparation method and application
  • An angiotensin-converting enzyme assay kit and its preparation method and application
  • An angiotensin-converting enzyme assay kit and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] An angiotensin-converting enzyme assay kit, comprising reagent R1 and reagent R2.

[0047] The components of reagent R1 are:

[0048]

[0049] The components of reagent R2 are:

[0050]

[0051]

[0052] The pH of reagent R1 is 7.5; the pH of reagent R2 is 8.2;

[0053] (1) Preparation of reagent R1: Take an appropriate amount of water, add the raw materials shown in R1 in turn, stir well to dissolve one raw material, then add the next raw material, adjust to the required pH value with hydrochloric acid or sodium hydroxide, and dilute to the required volume.

[0054](2) Preparation of reagent R2: Take an appropriate amount of water, add the raw materials shown in R2 in turn, stir well to dissolve one raw material, then add the next raw material, adjust the pH value with hydrochloric acid or sodium hydroxide, and dilute to the required volume.

Embodiment 2

[0056] The components of reagent R1 are:

[0057]

[0058] The components of reagent R2 are:

[0059]

[0060] The pH of reagent R is 7.8; the pH of reagent R2 is 8.2.

[0061] The preparation method is the same as in Example 1.

Embodiment 3

[0063] The components of reagent R1 are:

[0064]

[0065]

[0066] The components of reagent R2 are:

[0067]

[0068] The pH of reagent R was 8.3 and the pH of reagent R2 was 8.5.

[0069] The preparation method is the same as in Example 1.

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Abstract

The invention provides an angiotensin converting enzyme assay kit, the kit contains a reagent R1 and a reagent R2, the reagent R1 comprises the following components: N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid, sodium chloride, an ion activator, polyglycerol fatty acid ester, 4-hydroxy 2, 2, 6, 6-tetramethyl-1-oxo-piperidine (AAQ-2) and a preservative; and the reagent R2 is preparedfrom the following components: N-tri (hydroxymethyl) methyl-3-aminopropanesulfonic acid, FAPGG, a stabilizer and a preservative. The invention also provides a preparation method and application of thekit. The kit is a liquid kit with strong anti-interference capability, strong stability and good repeatability.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent assay, in particular to an angiotensin-converting enzyme assay kit and its preparation method and application. Background technique [0002] Angiotensin converting enzyme (ACE, EC3.4.15.1) is a Zn ubiquitous in mammalian tissues 2 +-dependent carboxydipeptidase, a membrane-integrated single-chain glycoprotein, mainly exists in pulmonary vascular endothelial cells, and can also be found in most tissues such as uterus, placenta, heart, kidney, brain, and adrenal cortex. Through the renin-angiotensin system RAS (rennin-angiotensin system) and kallikrein-kinin system, ACE plays an important role in regulating blood pressure, electrolyte and fluid balance, cardiovascular system development and structural remodeling. in Zn 2+ In the presence of conditions, ACE can hydrolyze the peptide bond between Phe8-His9 in the decapeptide angiotensin I (angiotensin I, Ang I), to produce the octapeptid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/37
CPCC12Q1/37
Inventor 刘安娜王洪星
Owner 中科梅奥(山东)医学检验有限公司
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