The invention discloses a functional sports drink and a preparation method thereof. The functional sports drink comprises the following components: sweetener, creatine pyruvate, taurine, lysine, vitamin C, sodium chloride and/or sodium citrate, food flavor and purified water. The preparation method comprises the following steps: adding sweetener in a defined amount of purified water to dissolve, filtering; adding creatine pyruvate, taurine, lysine, vitamin C, sodium chloride and/or sodium citrate and food flavor in a defined amount of purified water in turn to dissolve, filtering; mixing the filtrates, performing constant volume process with purified water to obtain a 1000ml of finished solution; and sterilizing to obtain the functional sports drink. By using the functional sports drink, the fluid balance can be regulated, the exercise tolerance can be improved, the organism anti-fatigue ability can be increased, and the recovery of the organism from fatigue after exercise can be promoted.