Serum apolipoprotein A1 determination kit, preparation method and application thereof

Abstract

The invention provides an apolipoprotein A1 (ApoA1) determination kit, which contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a 4-hydroxyethylpiperazineethanesulfonic acid (HEPES) buffer solution, NaCl, guanidine hydrochloride, sodium decyl sulfate, tetramethylurea, polyethylene glycol 6000, a surfactant and a preservative, and the reagent R2 is prepared from a 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, goat anti-human ApoA1 antibody coated latex particles, a surfactant, a stabilizer and a preservative. The invention also provides a preparation method and application of the kit, wherein the kit is beneficial to exposure of antigen sites in lipoprotein and promotion of antigen-antibody reaction due to mutualsynergy of multiple components, and is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

Description

technical field [0001] The present invention relates to the technical field of biochemical reagent assay, in particular to an apolipoprotein A1 assay kit, and also relates to a preparation method and application of the apolipoprotein A1 assay kit. Background technique [0002] Apolipoprotein A1 (apolipoprotein A1, ApoA1) is the main component of human serum high-density lipoprotein and is expressed in large quantities in serum. The main function of ApoA1 is to promote the transport of lipids, regulate enzyme activity, and guide the binding of plasma lipoproteins to cell surface receptors in lipoprotein metabolism. It can activate lecithin cholesterol acyltransferase to transport and decompose cholesterol in the liver. It plays an important role in the process of fat metabolism in the body and is an indicator of anti-atherosclerosis. Therefore, the reduction of ApoA1 can promote the formation of atherosclerosis. [0003] The main reason for the low level of apolipoprotein A...

AI Technical Summary

Problems solved by technology

Double-antibody sandwich immunochemiluminescence assay (ILMA) has high accuracy and sensitivity, but the equipment is expensive, the reagents are inconvenient to store, and the price is high

Latex immunoturbidimetric method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. However, the existing Chinese-made kits have low analytical sensitivity, poor stability, and incomplete antibody-antigen binding. Shortcomings, the present invention improves on the shortcoming of existing ApoA1 kit to meet the requirements of clinical detection and chemical analysis

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