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A stable serum pepsinogen I assay kit and its preparation method and application

A technology of pepsinogen and reagent kits, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve problems such as inability to quantify, expensive instruments and equipment, and inconvenient storage of reagents

Active Publication Date: 2021-05-18
中拓医学检验有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Double-antibody sandwich immunochemiluminescence assay (ILMA) has high accuracy and sensitivity, but the equipment is expensive, reagents are inconvenient to store, and the price is high; colloidal gold colorimetry is simple to operate and produces fast results, but it can only be used for qualitative research and cannot Quantitative
Latex immunoturbidimetric method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. ) impact and other shortcomings, the present invention improves the shortcomings of the existing pepsinogen I kit to meet the requirements of clinical testing and chemical analysis

Method used

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  • A stable serum pepsinogen I assay kit and its preparation method and application
  • A stable serum pepsinogen I assay kit and its preparation method and application
  • A stable serum pepsinogen I assay kit and its preparation method and application

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Embodiment 1

[0051] A conventional pepsinogen I assay kit, which includes reagent R1 and reagent R2.

[0052] Reagent R1 contains the following ingredients:

[0053] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0054] NaCl 9g / L;

[0055] Sodium hydroxide 0.1g / L;

[0056] Propylene glycol fatty acid ester 1%;

[0057] Polyoxyethylene Sorbitan Hexa Stearate 1%;

[0058] proclin300 1 ml / L.

[0059] Reagent R1 has a pH value of 7.2.

[0060] Reagent R2 contains the following ingredients:

[0061] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0062] Mouse anti-human PGI antibody coated latex particles 30mg / L;

[0063] Bovine serum albumin (BSA) 5g / L;

[0064] Polyvinylpyrrolidone k30 5g / L;

[0065] Mannitol 5g / L;

[0066] Glycerol 10ml / L;

[0067] Triton 100 1%;

[0068] proclin300 1ml / L.

[0069] Reagent R2 has a pH of 6.8.

[0070] Wherein said percentage is volume ratio.

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Abstract

The invention provides a pepsinogen I (PGI) assay kit, the kit contains reagent R1 and reagent R2; reagent R1 contains the following components: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, NaCl , sodium hydroxide, surfactants, preservatives. Reagent R2: piperazine-1,4-diethanesulfonic acid (PIPES) buffer, mouse anti-human PGI antibody coated latex particles, suspending agent, stabilizer, preservative. The invention also provides the preparation method and application of the kit. The kit can effectively avoid the interference of chylomicrons, and adopts different kinds of protective agents and suspending agents at the same time. It has strong stability, high sensitivity and good repeatability. Low cost liquid kit.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent assay, in particular to a pepsinogen I assay kit, and also to a preparation method and application of the pepsinogen I assay kit. Background technique [0002] Pepsinogen (pepsinogen), synthesized by the main cells of the oxyntic gland, becomes pepsin in the gastric cavity through the action of hydrochloric acid (HCL) or active pepsin (pepsin), and decomposes protein into fat, peptone and a small amount of peptides . The optimum pH for the enzyme action is 2, and the enzyme activity is lost after entering the small intestine. [0003] Pepsinogen is the precursor of pepsin. According to its biochemical properties and immunogenicity, it is divided into 2 subgroups. The immunogenicity of components 1-5 is the same, called pepsinogen I, which is mainly composed of fundic glands. Secreted by chief cells and mucus neck cells; components 6 and 7 are called pepsinogen II, in addition to bein...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/573G01N33/543G01N33/539
CPCG01N33/539G01N33/54313G01N33/573
Inventor 刘安娜
Owner 中拓医学检验有限公司
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