Serum iron determination kit and preparation method and application thereof
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A kit and serum iron technology, which is applied in biological testing, material testing products, etc., can solve the problems of unsatisfactory sensitivity and specificity of reagents, and achieve the effects of high sensitivity, high accuracy and convenient use.
Active Publication Date: 2019-04-12
中拓生物有限公司
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Problems solved by technology
Chromogens widely used clinically, such as ferrous azine, furan triazine disodium salt (ferene), bathophenanthroline (BPZ), tripyridyl triline (TPZ), etc., the most commonly used chromogen is ferrous azine (such as The kit disclosed in CN 106814063 A), but the reagents generally have the disadvantages of sensitivity and specificity that are not ideal. The present invention improves the shortcomings of the existing serum iron determination kit to meet the requirements of clinical detection and chemical analysis. Require
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Embodiment 1
[0039] A conventional kit for measuring serum iron, which includes reagent R1 and reagent R2.
[0040] Reagent R1:
[0041] Glycine buffer 60mmol / L
[0042] Ascorbic acid 35 g / L
[0043] Thiourea 20mmol / L
[0044] Hydroxylamine hydrochloride 30 mmol / L
[0045] Tween-20 1 g / L
[0046] Sodium azide 1 g / L
[0047] pH 5
[0048] Reagent R2:
[0049] Glycine buffer 50mmol / L
[0050] Saphenanthroline 2 g / L
[0051] pH 3
[0052] The reagents R1 and R2 described in this example need to prepare buffer solution first, adjust the pH with hydrochloric acid and sodium hydroxide, adjust to an appropriate pH value, and then add other substances to dissolve to obtain the assay reagent.
Embodiment 2
[0054] Glycine buffer 60mmol / L
[0055] Ascorbic acid 35 g / L
[0056] Thiosemicarbazide 30mmol / L
[0057] Hydroxylamine hydrochloride 35 mmol / L
[0058] Tween-20 1 g / L
[0059] Sodium azide 1 g / L
[0060] pH 5
[0061] Reagent R2:
[0062] Glycine buffer 40mmol / L
[0063] Saphenanthroline 3g / L
[0064] pH 3
[0065] The preparation method is the same as in Example 1.
Embodiment 3
[0067] Acetate buffer 50 mmol / L
[0068] Ascorbic acid 35 g / L
[0069] Thiosemicarbazide 40mmol / L
[0070] Sodium metabisulfite 35 mmol / L
[0071] Tween-20 1 g / L
[0072] Sodium azide 1 g / L
[0073] pH 5.5
[0074] Reagent R2:
[0075] Glycine buffer 45mmol / L
[0076] Saphenanthroline 3g / L;
[0077] pH 2.5
[0078] The preparation method is the same as in Example 1.
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Abstract
The invention discloses a serum iron determination kit. The kit comprises a reagent R1 and a reagent R2. The reagent R1 comprises a buffer solution, ascorbic acid, a de-interfering agent, a stabilizer, a surfactant, and a preservative. The reagent R2 comprises a buffer solution and bathophenanthroline disulfonic acid disodium salt. The invention further relates to a preparation method and application of the serum iron determination kit. The stable serum iron is a liquid double-reagent, reconstitution preparation is not required, and therefore the stable serum iron can be directly used when taken out from a bottle. The stabilizer is added in the reagent R1 to facilitate stability and reducibility maintenance of the ascorbic acid. The de-interfering agent is added in the reagent R1, thioureais mixed with a ascorbic acid solution, and therefore the R1 can be adopted as a masking agent as well as a reducing agent, and interference from other ions can be avoided. The reagent has high sensitivity. The kit is convenient to use, is high in specificity, is high in accuracy, is free of pollution to biochemical instruments, and can be further popularized in the market.
Description
technical field [0001] The invention relates to the field of medical immunological in vitro diagnostic reagents, in particular to a kit for measuring serum iron, and also relates to a preparation method and application of the kit for measuring serum iron. Background technique [0002] Iron is one of the most abundant essential trace elements in the human body. It is the main raw material for the synthesis of hemoglobin in red blood cells and is widely involved in the metabolic process in the human body. The average amount of iron in a normal adult is about 3-4.5g. About 70% of all the iron in the whole body exists in hemoglobin and a small amount exists in myoglobin, while the iron in the transport state of various enzymes and plasma accounts for only 2% of the iron in the whole body. In very small portions, iron deficiency or excess iron can cause various diseases. Transferrin, also known as transferrin (TRF, siderophilin), is the main iron-containing protein in plasma, wh...
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