The invention belongs to the field of
radiation biological
dose meters, and particularly relates to a characteristic project
estimation technique using serum iron and serum
copper as a marker. The technique includes the steps of measuring the concentration of the serum iron and the concentration of the serum
copper of a radiated mouse respectively by using a 2-(5-
bromine-2-
pyridine azobenzene)-5-2-diethylaminophenol (5-Br-PADAP) spectrophotometric method, calculating the ratio of the serum iron and the serum
copper, and then establishing a
dose-response curve between the serum iron / serum copper and a
radiation dose. According to the established dose-response curve, the dose response range of the biological
dose estimation technique is 0.5-7Gy, operation and index analysis are convenient, information of the
radiation dose can be acquired in 30 minutes, and the requirement for biological dose reconstruction can be met. Due to the fact that the technique has the advantages that the dose response is good, detection is convenient and
mass detection can be carried out, the technique has broad application prospects in biological
dose estimation for
nuclear radiation accidents or professional radiated workers.