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A serum iron assay kit and its preparation method and application
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A technology for kits and serum iron, which is applied in the preparation of serum iron assay kits and the field of serum iron assay kits, can solve the problems of reagent sensitivity and specificity that are not ideal, and achieve high sensitivity, easy decomposition, and high specificity Effect
Active Publication Date: 2022-01-28
中拓生物有限公司
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Problems solved by technology
Chromogens widely used clinically, such as ferrous azine, furan triazine disodium salt (ferene), bathophenanthroline (BPZ), tripyridyl triline (TPZ), etc., the most commonly used chromogen is ferrous azine (such as The kit disclosed in CN 106814063 A), but the reagents generally have the disadvantages of sensitivity and specificity that are not ideal. The present invention improves the shortcomings of the existing serum iron determination kit to meet the requirements of clinical detection and chemical analysis. Require
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Embodiment 1
[0039] A conventional kit for measuring serum iron, which includes reagent R1 and reagent R2.
[0040] Reagent R1:
[0041] Glycine buffer 60mmol / L
[0042] Ascorbic acid 35 g / L
[0043] Thiourea 20mmol / L
[0044] Hydroxylamine hydrochloride 30 mmol / L
[0045] Tween-20 1 g / L
[0046] Sodium azide 1 g / L
[0047] pH 5
[0048] Reagent R2:
[0049] Glycine buffer 50mmol / L
[0050] Saphenanthroline 2 g / L
[0051] pH 3
[0052] The reagents R1 and R2 described in this example need to prepare buffer solution first, adjust the pH with hydrochloric acid and sodium hydroxide, adjust to an appropriate pH value, and then add other substances to dissolve to obtain the assay reagent.
Embodiment 2
[0054] Glycine buffer 60mmol / L
[0055] Ascorbic acid 35 g / L
[0056] Thiosemicarbazide 30mmol / L
[0057] Hydroxylamine hydrochloride 35 mmol / L
[0058] Tween-20 1 g / L
[0059] Sodium azide 1 g / L
[0060] pH 5
[0061] Reagent R2:
[0062] Glycine buffer 40mmol / L
[0063] Saphenanthroline 3g / L
[0064] pH 3
[0065] The preparation method is the same as in Example 1.
Embodiment 3
[0067] Acetate buffer 50 mmol / L
[0068] Ascorbic acid 35 g / L
[0069] Thiosemicarbazide 40mmol / L
[0070] Sodium metabisulfite 35 mmol / L
[0071] Tween-20 1 g / L
[0072] Sodium azide 1 g / L
[0073] pH 5.5
[0074] Reagent R2:
[0075] Glycine buffer 45mmol / L
[0076] Saphenanthroline 3g / L;
[0077] pH 2.5
[0078] The preparation method is the same as in Example 1.
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Abstract
The invention discloses a kit for measuring serum iron. The kit contains reagent R1 and reagent R2; the reagent R1 is composed of buffer, ascorbic acid, interference removing agent, stabilizer, surfactant and preservative; the reagent R2 The invention contains the following components: a buffer solution and saphenanthroline; and also relates to a preparation method and application of a kit for measuring serum iron. The stable serum iron of the present invention is a liquid double reagent, which does not need to be redissolved for preparation, and can be used directly after opening the bottle. By adding a stabilizer to the reagent R1, it is beneficial to maintain the stability and reducibility of ascorbic acid. Reagent R1 is added with interference removing agent, and thiourea is mixed with ascorbic acid solution, which can be used as reducing agent and masking agent to remove the interference of other ions. This reagent has higher sensitivity. The reagent is convenient to use, has high specificity, high accuracy, and has no pollution to biochemical instruments, which is conducive to the further promotion of the reagent in the market.
Description
technical field [0001] The invention relates to the field of medical immunological in vitro diagnostic reagents, in particular to a kit for measuring serum iron, and also relates to a preparation method and application of the kit for measuring serum iron. Background technique [0002] Iron is one of the most abundant essential trace elements in the human body. It is the main raw material for the synthesis of hemoglobin in red blood cells and is widely involved in the metabolic process in the human body. The average amount of iron in a normal adult is about 3-4.5g. About 70% of all the iron in the whole body exists in hemoglobin and a small amount exists in myoglobin, while the iron in the transport state of various enzymes and plasma accounts for only 2% of the iron in the whole body. In very small portions, iron deficiency or excess iron can cause various diseases. Transferrin, also known as transferrin (TRF, siderophilin), is the main iron-containing protein in plasma, wh...
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