Radiation biological dose estimation method based on serum iron/serum copper

A technology of radiation biological dose and serum iron, which is applied in the field of characteristic subject estimation, can solve the problems of high requirements for experimental technicians, long experimental time, and narrow response range, and achieves the effects of common test reagents, simple operation, and cumbersome operation.

Inactive Publication Date: 2013-08-28
NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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Problems solved by technology

[0007] Aiming at the problems of narrow dose response range, cumbersome operation, long experiment time and high requirements for experimental technicians in the existing biological dose estimation technology, the present invention develops a biological solution based on serum iron / serum copper that has a wide dose response range, fast and easy operation. Dose Estimation Techniques

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  • Radiation biological dose estimation method based on serum iron/serum copper
  • Radiation biological dose estimation method based on serum iron/serum copper
  • Radiation biological dose estimation method based on serum iron/serum copper

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Embodiment Construction

[0032] Estimation method of γ-ray biological dose based on mouse serum iron / serum copper

[0033] 1. Irradiation of mice:

[0034] Sixty male Kunming mice (18-22 g), 10 in each group, were irradiated with cobalt-60γ-rays of 0, 0.5, 1, 3, 5 and 7 Gy (unit: Gray) respectively. Radiation dose calibration was performed with Famar absolute dosimeter.

[0035] 2. Serum sample preparation of irradiated mice:

[0036] a) Anesthesia: intraperitoneal injection of pentobarbital sodium at a concentration of 1% (40mg / kg) in mice;

[0037]b) Blood collection by eyeball enucleation: fix the head of the mouse with the index finger and thumb, remove the eyeball of the mouse with tweezers, and collect peripheral blood using a disposable EP tube soaked in 10% HCl solution and fully rinsed with distilled water;

[0038] c) Standing: the collected peripheral blood was left standing at room temperature for 30 minutes;

[0039] d) Centrifugation: After standing still for 30 minutes, place the EP...

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Abstract

The invention belongs to the field of radiation biological dose meters, and particularly relates to a characteristic project estimation technique using serum iron and serum copper as a marker. The technique includes the steps of measuring the concentration of the serum iron and the concentration of the serum copper of a radiated mouse respectively by using a 2-(5-bromine-2-pyridine azobenzene)-5-2-diethylaminophenol (5-Br-PADAP) spectrophotometric method, calculating the ratio of the serum iron and the serum copper, and then establishing a dose-response curve between the serum iron / serum copper and a radiation dose. According to the established dose-response curve, the dose response range of the biological dose estimation technique is 0.5-7Gy, operation and index analysis are convenient, information of the radiation dose can be acquired in 30 minutes, and the requirement for biological dose reconstruction can be met. Due to the fact that the technique has the advantages that the dose response is good, detection is convenient and mass detection can be carried out, the technique has broad application prospects in biological dose estimation for nuclear radiation accidents or professional radiated workers.

Description

technical field [0001] The invention belongs to the field of radiation biological dosimeters, and in particular relates to a characteristic subject estimation technique using serum iron and serum copper as markers. Background technique [0002] With the wide application of nuclear technology in military and civilian fields, people pay more and more attention to radiation safety. Biological dose estimation technology is a method to calibrate the exposure dose of organisms by using radiation-sensitive markers in organisms. It is the basis for accurate triage of people around nuclear accidents, and it is also the basis for whether the suspected exposed people can obtain correct diagnosis, and then get timely treatment. The key to proper treatment. Biological dosimetry has the irreplaceable advantage of physical dosimetry because of its faithfulness, reproducibility and predictability of radiation injury prognosis. After more than 40 years of unremitting efforts, the biologica...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01T1/02G01N21/31
Inventor 张晓红张海黔闵璇宇胡晓丹娄志超张亚男
Owner NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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