Radiation biological dose estimation method based on serum iron/serum copper
A technology of radiation biological dose and serum iron, which is applied in the field of characteristic subject estimation, can solve the problems of high requirements for experimental technicians, long experimental time, and narrow response range, and achieves the effects of common test reagents, simple operation, and cumbersome operation.
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[0032] Estimation method of γ-ray biological dose based on mouse serum iron / serum copper
[0033] 1. Irradiation of mice:
[0034] Sixty male Kunming mice (18-22 g), 10 in each group, were irradiated with cobalt-60γ-rays of 0, 0.5, 1, 3, 5 and 7 Gy (unit: Gray) respectively. Radiation dose calibration was performed with Famar absolute dosimeter.
[0035] 2. Serum sample preparation of irradiated mice:
[0036] a) Anesthesia: intraperitoneal injection of pentobarbital sodium at a concentration of 1% (40mg / kg) in mice;
[0037]b) Blood collection by eyeball enucleation: fix the head of the mouse with the index finger and thumb, remove the eyeball of the mouse with tweezers, and collect peripheral blood using a disposable EP tube soaked in 10% HCl solution and fully rinsed with distilled water;
[0038] c) Standing: the collected peripheral blood was left standing at room temperature for 30 minutes;
[0039] d) Centrifugation: After standing still for 30 minutes, place the EP...
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