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A stable serum iron detection kit

A technology for detecting kits and serum iron, which can be used in the measurement of color/spectral characteristics, material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc. It can solve the problems of high iron toxicity, etc. To achieve the effect of enhanced stability, cheap price and convenient use

Active Publication Date: 2019-03-12
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extracellular iron bound to ferritin enters unstable iron pools in the cell through endocytosis, and this form of iron is highly toxic

Method used

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  • A stable serum iron detection kit
  • A stable serum iron detection kit
  • A stable serum iron detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A conventional kit for detecting serum iron, which includes reagent R1 and reagent R2.

[0030] Wherein the reagent R1 consists of:

[0031] pH 4.2 Tris buffer 60mmol / L

[0032] Ascorbic acid 100mmol / L

[0033] Sodium azide 10mmol / L

[0034] Surfactant TritonX-100 1%

[0035] Reagent R2 consists of:

[0036] pH 4.2 Tris buffer 60mmol / L

[0037] Ferrozine 50mmol / L

[0038] Sodium azide 10mmol / L

[0039] The reagent R1 and reagent R2 described in this embodiment need to be prepared by adding the buffer substance Tris first, and then adding other substances after adjusting the pH to an appropriate pH value with hydrochloric acid. The test kit described in this embodiment, when in use, is to use Mindray 800 full-automatic biochemical analyzer with double reagent function, and utilize the endpoint method to measure, the operation is as follows:

[0040] Add 20 μl of physiological saline, sample or calibrator, then add 200 μl of R1 reagent for pre-incubation for 5 min...

Embodiment 2

[0045] Accuracy verification test: the serum iron detection reagent of Example 1 was used as the experimental group, and a serum iron detection kit with good accuracy and stability recognized on the market was used as the control group for detection, and 20 clinical serum samples were tested The test was carried out, and the test results are shown in Table 1. Correlation curves for the two reagents were obtained (eg figure 1 Shown), the results show that the correlation coefficient of the two kits is 0.9989, indicating that the correlation between the two is relatively good. It proves that the added and changed components of the kit of the present invention will not affect its accuracy, and the kit still maintains a good accuracy.

[0046] Table 1 Example 1 reagent and market-approved serum iron detection kit comparative detection results

[0047]

Embodiment 3

[0049]Linear correlation verification test: Prepare a high-value sample with an iron content of 180 μmol / L, serially dilute it with normal saline, and prepare 6 samples with different concentrations, the concentrations are 180 μmol / L, 144 μmol / L, 108 μmol / L, 72 μmol / L L, 36 μmol / L, 0 μmol / L. The reagents of Example 1 and the control group were used for detection respectively. The samples of each concentration were measured three times, and the average values ​​were taken respectively. The detection results are shown in Table 2.

[0050] Table 2 The detection results of the reagent linear correlation verification experiment in Example 1

[0051]

[0052] As shown in the above table, the correlation coefficients of embodiment 1 and the detection results of the control group reagents are all greater than 0.990, and the correlation coefficients of the detection results of the reagents of embodiment 1 are slightly greater than the correlation coefficients of the detection result...

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Abstract

The invention discloses a serum iron ferrous pyrazine colorimetric detection kit, and belongs to the technical field of clinical in vitro detection reagents. The kit is prepared from a reagent R1 and a reagent R2. Buffer solutions of the reagent R1 and the reagent R2 are replaced as 60mM of Tris buffer solution with pH of 4.2, and then a surfactant of the reagent R1 is replaced as TrixtonX-100. The stability of the kit is improved, the linear dependence is better, the accuracy of the reagents are good, and the further popularization and use in the market are benefited.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a serum iron immunoturbidimetric detection kit. Background technique [0002] Iron is one of the main trace elements in the human body, and it is often combined with transferrin in the form of ferric ions and transported. Iron plays an important role in the process of cell metabolism, and an appropriate amount of iron plays an important role in oxygen transport in biological organisms, biological oxidation of cells, bacterial pathogenicity, control of oxygen free radicals, DNA replication in cells, and cell reproduction. In addition, iron ions can also participate in the synthesis of hemoglobin, myoglobin and essential enzymes in the human body, and play a vital role in human metabolism and various physiological activities. [0003] Under normal circumstances, the human body maintains a balance between the amount of iron excreted by the human body th...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 谭柏清李敏甘宜梧包兴艳
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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