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Total iron binding force detection kit and preparation method thereof

A detection kit and iron binding force technology, applied in the biological field, can solve the problems of difficult popularization, cumbersome operation steps, and high detection cost

Inactive Publication Date: 2019-12-13
苏州普瑞斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The atomic absorption method not only requires special expensive instruments, but also requires precipitation and centrifugation. The detection cost is high, the operation steps are cumbersome, and it is difficult to popularize clinically.

Method used

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  • Total iron binding force detection kit and preparation method thereof
  • Total iron binding force detection kit and preparation method thereof
  • Total iron binding force detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of total iron binding capacity detection kit of the present invention comprises reagent R1 and reagent R2, each component and concentration of described reagent R1 comprise: Tris buffer solution, its concentration is 80mmol / L, thiourea, its concentration is 30mmol / L, hydroxylamine hydrochloride, its concentration is 150mmol / L, ferrous ammonium sulfate, its concentration is 5mmol / L, TritonX-100, its concentration is 0.2%, Proclin-950, its concentration is 0.8ml / L;

[0025] The concentration of each component of the reagent R2 includes: Tris buffer solution, whose concentration is 30mmol / L, ferrozine, whose concentration is 20mmol / L, ascorbic acid, whose concentration is 10mmol / L, Proclin-950, whose concentration is 0.8 ml / L.

[0026] The pH value of the reagent R1 is 4.00.

[0027] The pH value of the reagent R2 is 7.00.

[0028] A preparation method of a total iron binding capacity detection kit, comprising the following steps: A), preparation of reagent R1: ...

Embodiment 2

[0035] A kind of total iron binding capacity detection kit of the present invention comprises reagent R1 and reagent R2, each component and concentration of described reagent R1 comprise: Tris buffer solution, its concentration is 120mmol / L, thiourea, its concentration is 45mmol / L, hydroxylamine hydrochloride, its concentration is 220mmol / L, ferrous ammonium sulfate, its concentration is 20mmol / L, TritonX-100, its concentration is 1.0%, Proclin-300, its concentration is 1.2ml / L;

[0036] The concentration of each component of the reagent R2 includes: Tris buffer solution, whose concentration is 55mmol / L, ferrozine, whose concentration is 35mmol / L, ascorbic acid, whose concentration is 30mmol / L, Proclin-300, whose concentration is 1.2 ml / L.

[0037] The pH value of the reagent R1 is 4.5.

[0038] The pH value of the reagent R2 is 7.6.

[0039] A preparation method of a total iron binding capacity detection kit, comprising the following steps: A), preparation of reagent R1: ...

Embodiment 3

[0046] A total iron binding capacity detection kit of the present invention comprises reagent R1 and reagent R2, each component and concentration of described reagent R1 comprises: Tris buffer solution, its concentration is 160mmol / L, thiourea, its concentration is 60mmol / L, hydroxylamine hydrochloride, its concentration is 300mmol / L, ferrous ammonium sulfate, its concentration is 30mmol / L, TritonX-100, its concentration is 2.0%, thimerosal, its concentration is 2.0ml / L;

[0047] The concentration of each component of the reagent R2 includes: Tris buffer solution, whose concentration is 80mmol / L, ferrozine, whose concentration is 50mmol / L, ascorbic acid, whose concentration is 50mmol / L, thimerosal, whose concentration is 2.0ml / L L.

[0048] The pH value of the reagent R1 is 5.00.

[0049] The pH value of the reagent R2 is 8.30.

[0050] A preparation method of a total iron binding capacity detection kit, comprising the following steps: A), preparation of reagent R1:

[005...

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Abstract

The invention belongs to the technical field of biology and belongs to a total iron binding force detection kit, in particular, a preparation method of a total iron binding force detection kit. The preparation method involves the preparation of a reagent R1 and the preparation of a reagent R2; a solution preparation tank is arranged on a magnetic stirrer; a solution is made to keep a medium-speedrotating state; a Tris buffer solution, ferrous azine, ascorbic acid and a second buffer solution are added when stirring is performed; stirring is performed for 30 minutes until the materials are completely dissolved, and the solution is clear and transparent and has no precipitate at the bottom of the solution preparation tank; and volume fixing is carried out, so that the volume of the solutioncan be a final volume. According to the detection kit, a spectrophotometric method is adopted to detect a total iron binding force with the ferrous azine used as a substrate; serum iron combined withtransferring, in an acidic medium, is dissociated from the transferrin; and the serum iron is reduced into Fe <2+> by a reducing agent, the Fe <2+> and the ferrous azine generate a purplish red compound; a absorbance value at 562nm is detected by a spectrophotometer, so that total iron content in serum and plasma samples can be detected; and the linear range of the kit can reach 50-400 micromole / L.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a total iron binding capacity detection kit, in particular to a preparation method of the total iron binding capacity detection kit. Background technique [0002] The determination of total iron-binding capacity is a routine test item. Numerous clinical data show that iron-deficiency anemia, acute hepatitis and other diseases lead to a decrease in total iron-binding capacity, while liver cirrhosis, kidney disease, uremia and hemochromatosis and other diseases can lead to total iron-binding capacity. The iron binding capacity increases, and the determination of the total iron binding capacity has important clinical value for the diagnosis of related diseases and the observation of treatment effects. [0003] At present, the clinical determination of total iron binding capacity mainly adopts the atomic absorption method adsorbed by light magnesium carbonate and various complex photometry...

Claims

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Application Information

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IPC IPC(8): G01N33/90
CPCG01N33/90
Inventor 袁嘉扬张凤
Owner 苏州普瑞斯生物科技有限公司
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