Traditional Chinese medicine composition for preventing and treating inflammatory anemia
A composition and inflammatory technology, applied in the field of biomedicine, to achieve the effect of broadening the scope of indications and achieving significant preventive and therapeutic effects
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Embodiment 1
[0023] Example 1 Lipopolysaccharide-induced inflammatory anemia model experiment in mice
[0024] 1. Materials and methods:
[0025] 1. Chinese medicine prescription: Chinese medicine composition includes 100 parts of angelica, 100 parts of astragalus, 30 parts of donkey-hide gelatin, 150 parts of motherwort, and 3 parts of tangerine peel. The preparation method is as described above, and it is prepared into pills.
[0026] 2. Animals C57B6 male mice, 8 weeks old, were randomly divided into four groups according to body weight after one week of adaptive feeding, which were blank control group (CON), traditional Chinese medicine group (AA), lipopolysaccharide group (LPS), and lipopolysaccharide group (LPS). The polysaccharide was dissolved in PBS, and the lipopolysaccharide and traditional Chinese medicine group (LPS+AA), 5 rats in each group.
[0027] The mice in each group were given medicine, the blank control group was given PBS, the Chinese medicine group was administere...
Embodiment 2
[0048] Example 2 Freund's complete adjuvant-induced inflammatory anemia pharmacological and pharmacodynamic experiments in rats
[0049] 1. Experimental materials
[0050] Experimental animals: male SD rats, 150±2g, 6-8 weeks old, commercially available.
[0051] Chinese medicine prescription is the same as embodiment 1.
[0052] 2. Animal feeding and grouping
[0053]After one week of adaptive feeding, male SD rats were weighed and marked with picric acid. Five rats were fed in each cage. The bedding in the cage was replaced every two days and wiped and disinfected with 75% alcohol. The temperature of the animal room was 23±2°C, and the relative humidity was 50±20%, natural lighting. All rats were free to eat and drink, and all operations strictly followed the "Regulations on the Management of Experimental Animals".
[0054] The model was established by subcutaneously injecting 0.15 mL of Mycobacterium tuberculosis with Freund's complete adjuvant at a concentration of 10 ...
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