Serum apolipoprotein B assay kit, preparation method and application thereof

Abstract

The invention provides an apolipoprotein B (ApoB) assay kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components: a 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, NaCl, guanidine hydrochloride, sodium decyl sulfate, tetramethylurea, polyethylene glycol 6000, a surfactant and a preservative; and the reagent R2 is prepared from the 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, goat anti-human ApoB antibody coated latex particles, the surfactant, a stabilizer and the preservative. The invention also provides a preparation method and an application of the kit, and the kit is beneficial to exposure of antigen sites in lipoprotein and promotion of an antigen-antibody reaction due to mutualsynergy of multiple components, and is a liquid kit with strong stability, high sensitivity, good repeatability and low cost.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent determination, in particular to an apolipoprotein B assay kit, and also relates to a preparation method and application of the apolipoprotein B assay kit. Background technique [0002] Apolipoprotein is the protein part of plasma lipoprotein, which can bind and transport blood lipids to various tissues of the body for metabolism and utilization. A large number of studies have found that mutations in the apolipoprotein gene form polymorphisms of different allele types, and further form apolipoproteins of different phenotypes, which can affect blood lipid metabolism and utilization, thereby affecting hyperlipidemia, atherosclerosis, The occurrence and development of cardiovascular and cerebrovascular diseases. [0003] Apolipoprotein B (apolipoprotein B, ApoB) exists on the surface of low-density lipoprotein, and about 90% of apolipoprotein B is not distributed in low-density lipoprotei...

AI Technical Summary

Problems solved by technology

Double-antibody sandwich immunochemiluminescence assay (ILMA) has high accuracy and sensitivity, but the equipment is expensive, the reagents are inconvenient to store, and the price is high

Latex immunoturbidimetric method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. However, the existing Chinese-made kits have low analytical sensitivity, poor stability, and incomplete antibody-antigen binding. Shortcomings, the present invention improves on the shortcoming of existing ApoB test kits to meet the requirements of clinical detection and chemical analysis

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