Preparation method of liquid calibrator of fructosamine determination kit

A kit and the technology of fructosamine, applied in the field of preparation of liquid calibrator of fructosamine determination kit, can solve the problems of hidden dangers in biological safety, inconvenient use, high price, etc., and achieve the reduction of biological safety risk and long-term stability Good effect of reducing energy consumption and cost

Pending Publication Date: 2022-03-01
威海威高生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned calibrator for determining fructosamine has the following disadvantages: (1) glycosylated human serum albumin requires human serum albumin as a reaction substrate, which has biological safety hazards; (2) 1-deoxy-1-morpholine-D-fructose and 1- Deoxy-1-piperidine-D-fructose is expensive, which increases production costs; (3) pentaacetate glucolipid is not easily soluble in water, and acetone needs to be added when dissolving, but acetone is volatile and affects the concentration
[0007] Among the above methods, the disadvantages of method 1 are: the use o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of liquid calibrator of fructosamine determination kit
  • Preparation method of liquid calibrator of fructosamine determination kit
  • Preparation method of liquid calibrator of fructosamine determination kit

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] A preparation method of a fructum amine determination kit liquid calibration, including the following steps:

[0025] (1) Sugarization process: Take 40 g of bovine serum albumin is dissolved in phosphate buffer in 1 l0.2 mol / L, the pH of the phosphate buffer is 7.5, and 5 g of glucose is completely dissolved, and the 10 ° C water bath is 1000R / min. Stir, 8h, place it into the refrigerator. Place 12h at 2 ° C;

[0026] (2) Removal of the glucose process: 1 ku of glucose oxidase, 25 ° C water bath 200R / min was stirred for 2 h, diluted with 0.2 l0.2 mol / L of phosphate buffer.

[0027] (3) Dispensl: 1 g of sodium azide sodium was added after the reaction was completed, and was stored as 1 ml / branch, and 2-8 ° C was stored.

Example Embodiment

[0028] Example 2

[0029] A preparation method of a fructum amine determination kit liquid calibration, including the following steps:

[0030] (1) Swegulation: Take the blood-free protein 20g of the sheep in a phosphate buffer dissolved in 1 L0.2 mol / L, the pH of the phosphate buffer is 7.5, and 2.5 g of glucose is completely dissolved, 1200 r / min under 60 ° C water bath. Stir for 10 h, placed in a refrigerator, 20 h at 4 ° C, resulting in the reagent liquid A;

[0031] (2) Removal of glucose: Take the reagent liquid from the refrigerator to add glucose oxidase 0.5 ku, 35 ° C, and 500r / min in the water bath is stirred with 0.5 h, diluted with 0.1 l0.2 mol / L of phosphate buffer;

[0032] (3) Sub-installation: Step (2) After the reaction, 0.6 g of potassium sorbate was added, and it was set to 1 mL / branch, 2-8 ° C.

Example Embodiment

[0033] Example 3

[0034] A preparation method of a fructum amine determination kit liquid calibration, including the following steps:

[0035] (1) Sweets: Take 55 g of rabbit serum albumin in 1 L0.2 mol / L of phosphate buffer, pH of phosphate buffer is 7.5, and 7 g of glucose is completely dissolved, 1500 r / min under 60 ° C water bath is stirred. 8h, placed in a refrigerator, 8 h at 3 ° C, resulting in reagent liquid a;

[0036] (2) Removal of glucose: Take the reagent liquid A from the refrigerator to add glucose oxidase 2Ku, 30 ° C water bath, and 500r / min in the water bath for 3 h, diluted with 0.35 L0.2 mol / L of phosphate buffer;

[0037] (3) Dispensl: Step (2) After the reaction, 1.5 g of methylisothiazolinone was added, and the dispensing was 1 ml / branch, and 2-8 ° C was stored.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of biological detection, in particular to a preparation method of a liquid calibrator of a fructosamine determination kit. The method comprises the following steps: (1) saccharifying: dissolving animal serum albumin in a 0.05-2 mol/L phosphate buffer solution until the concentration of the animal serum albumin reaches 10-60 g/L, adding glucose until the concentration of the glucose reaches 1.25-7.5 g/L, stirring for 6-10 hours at the speed of 800-2000 r/min under the water bath condition of 40-70 DEG C, and placing in a refrigerator for 5-24 hours to obtain a reagent solution A; (2) removing glucose; and (3) sub-packaging. The method is high in safety, low in raw material consumption, high in yield and low in time consumption, residual glucose is removed by using a biological method, freeze-drying and dialysis are not needed, and the long-term stability is good when the method is stored at 2-8 DEG C.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for preparing a liquid calibrator of a fructosamine assay kit. Background technique [0002] Serum fructosamine is a polymer ketamine structure formed by non-enzymatic glycosylation of glucose and serum protein, also known as glycosylated serum protein. Its concentration is positively correlated with blood sugar level and relatively stable. Since the half-life of serum protein is 17-20 days, fructosamine can reflect the average blood sugar level within 2-3 weeks before the test, and the determination of fructosamine can assist in the diagnosis, condition monitoring and curative effect observation of diabetes. [0003] The fructosamine assay kit (tetrazolium blue method) is used for the quantitative detection of fructosamine (FMN) in human serum samples in vitro. The currently reported calibration substances for the determination of fructosamine are: glycosy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/28
CPCG01N1/28G01N2001/2893
Inventor 黄周亮姚继承饶志明于士杰张秋霞
Owner 威海威高生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap