Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of liquid calibrator of fructosamine determination kit

A kit and the technology of fructosamine, applied in the field of preparation of liquid calibrator of fructosamine determination kit, can solve the problems of hidden dangers in biological safety, inconvenient use, high price, etc., and achieve the reduction of biological safety risk and long-term stability Good effect of reducing energy consumption and cost

Pending Publication Date: 2022-03-01
威海威高生物科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned calibrator for determining fructosamine has the following disadvantages: (1) glycosylated human serum albumin requires human serum albumin as a reaction substrate, which has biological safety hazards; (2) 1-deoxy-1-morpholine-D-fructose and 1- Deoxy-1-piperidine-D-fructose is expensive, which increases production costs; (3) pentaacetate glucolipid is not easily soluble in water, and acetone needs to be added when dissolving, but acetone is volatile and affects the concentration
[0007] Among the above methods, the disadvantages of method 1 are: the use of human serum albumin, potential biosafety risks; the use of raw materials is high, the yield is low, and the process takes a long time; dialysis is required, and a large amount of raw materials are wasted; freeze-dried and freeze-dried products are required Reconstitution is required during use, which increases the inconvenience of use, and is prone to instability after reconstitution, large energy consumption and high cost
The disadvantages of method two are: high raw material usage, low yield, long time-consuming, human serum albumin is used, and potential biosafety risks

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of liquid calibrator of fructosamine determination kit
  • Preparation method of liquid calibrator of fructosamine determination kit
  • Preparation method of liquid calibrator of fructosamine determination kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method for a fructosamine assay kit liquid calibrator, comprising the steps:

[0025] (1) Saccharification process: Take 40g of bovine serum albumin and dissolve it in 1L of 0.2mol / L phosphate buffer, the pH of the phosphate buffer is 7.5, add 5g of glucose to dissolve completely, and then set the temperature at 1000r / min in a water bath at 60°C Stir for 8h and place in the refrigerator. Place at 2°C for 12 hours;

[0026] (2) Glucose removal process: Add 1KU of glucose oxidase, stir in a water bath at 25°C at 200r / min for 2h, and dilute with 0.2L of 0.2mol / L phosphate buffer.

[0027] (3) Subpackaging: Add 1 g of sodium azide after the reaction, subpackage into 1 mL / tube, and store at 2-8°C.

Embodiment 2

[0029] A preparation method for a fructosamine assay kit liquid calibrator, comprising the steps:

[0030] (1) Saccharification: Take 20g of sheep serum albumin and dissolve it in 1L of 0.2mol / L phosphate buffer, the pH of the phosphate buffer is 7.5, add 2.5g of glucose to dissolve completely, and set the temperature at 1200r / min in a water bath at 60°C Stir for 10 hours, place in the refrigerator, and place at 4°C for 20 hours to obtain reagent solution A;

[0031] (2) Glucose removal: Take out reagent solution A from the refrigerator and add 0.5KU of glucose oxidase, in a water bath at 35°C, stir at 500r / min for 0.5h during the water bath, and dilute with 0.1L of 0.2mol / L phosphate buffer;

[0032] (3) Packing: Add 0.6 g of potassium sorbate after the reaction in step (2), pack into 1 mL / bottle, and store at 2-8°C.

Embodiment 3

[0034] A preparation method for a fructosamine assay kit liquid calibrator, comprising the steps:

[0035] (1) Saccharification: Dissolve 55g of rabbit serum albumin in 1L of 0.2mol / L phosphate buffer, the pH of the phosphate buffer is 7.5, add 7g of glucose to dissolve completely, and stir at 1500r / min in a water bath at 60°C 8h, put it in the refrigerator, and place it at 3°C ​​for 8h to get reagent solution A;

[0036] (2) Glucose removal: take out reagent solution A from the refrigerator and add glucose oxidase 2KU, in a water bath at 30°C, stir at 500r / min for 3 hours during the water bath, and dilute with 0.35L of 0.2mol / L phosphate buffer;

[0037] (3) Packing: Add 1.5 g of methylisothiazolinone after the reaction in step (2), pack into 1 mL / bottle, and store at 2-8°C.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biological detection, in particular to a preparation method of a liquid calibrator of a fructosamine determination kit. The method comprises the following steps: (1) saccharifying: dissolving animal serum albumin in a 0.05-2 mol / L phosphate buffer solution until the concentration of the animal serum albumin reaches 10-60 g / L, adding glucose until the concentration of the glucose reaches 1.25-7.5 g / L, stirring for 6-10 hours at the speed of 800-2000 r / min under the water bath condition of 40-70 DEG C, and placing in a refrigerator for 5-24 hours to obtain a reagent solution A; (2) removing glucose; and (3) sub-packaging. The method is high in safety, low in raw material consumption, high in yield and low in time consumption, residual glucose is removed by using a biological method, freeze-drying and dialysis are not needed, and the long-term stability is good when the method is stored at 2-8 DEG C.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for preparing a liquid calibrator of a fructosamine assay kit. Background technique [0002] Serum fructosamine is a polymer ketamine structure formed by non-enzymatic glycosylation of glucose and serum protein, also known as glycosylated serum protein. Its concentration is positively correlated with blood sugar level and relatively stable. Since the half-life of serum protein is 17-20 days, fructosamine can reflect the average blood sugar level within 2-3 weeks before the test, and the determination of fructosamine can assist in the diagnosis, condition monitoring and curative effect observation of diabetes. [0003] The fructosamine assay kit (tetrazolium blue method) is used for the quantitative detection of fructosamine (FMN) in human serum samples in vitro. The currently reported calibration substances for the determination of fructosamine are: glycosy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/28
CPCG01N1/28G01N2001/2893
Inventor 黄周亮姚继承饶志明于士杰张秋霞
Owner 威海威高生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com