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A kit for measuring serum high-density lipoprotein cholesterol

A high-density lipoprotein and cholesterol technology, applied in the field of biochemical reagent determination, can solve problems such as poor anti-interference ability and poor stability, and achieve the effects of excellent stability, improved stability and convenient use.

Active Publication Date: 2021-08-31
中拓生物有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The current method for detecting high-density lipoprotein cholesterol is mainly the direct enzymatic method in biochemical reagents. This method has the advantages of high specificity, simple and fast operation, accuracy and safety, automatic analysis, and low cost. The cartridge has disadvantages such as poor stability and poor anti-interference ability. The present invention improves the shortcomings of the existing high-density lipoprotein cholesterol kit to meet the requirements of clinical detection and chemical analysis.

Method used

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  • A kit for measuring serum high-density lipoprotein cholesterol
  • A kit for measuring serum high-density lipoprotein cholesterol
  • A kit for measuring serum high-density lipoprotein cholesterol

Examples

Experimental program
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Effect test

Embodiment 1

[0055] A conventional kit for measuring high-density lipoprotein cholesterol, which includes reagent R1 and reagent R2.

[0056] Reagent R1 contains the following components:

[0057] 3-morpholine propanesulfonic acid buffer 50 mmol / L;

[0058] α-Cyclic glucan sulfate 0.5 mmol / L;

[0059] Dextran sulfate 0.5g / L;

[0060] Pullulan 0.5g / L;

[0061] Magnesium chloride 2 mmol / L;

[0062] Sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylanilide 0.5mmol / L;

[0063] Triton-100 1%;

[0064] proclin300 1ml / L;

[0065] Reagent R2 contains the following ingredients:

[0066]3-morpholine propanesulfonic acid buffer 50mmol / L;

[0067] Cholesterol esterase 100U / L;

[0068] Cholesterol oxidase 3KU / L;

[0069] Peroxidase enzyme 10KU / L;

[0070] 4-aminoantipyrine 0.5mmol / L;

[0071] Flavin adenine dinucleotide disodium 0.3g / L;

[0072] Bovine serum albumin 5g / L;

[0073] Trehalose 5g / L;

[0074] Triton-100 1%;

[0075] proclin300 1ml / L;

[0076] Wherein said percentage is vo...

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Abstract

The invention provides a high-density lipoprotein cholesterol assay kit, the kit contains reagent R1 and reagent R2; the reagent R1 contains the following components: 3-morpholine propanesulfonic acid (MOPS) buffer solution, α-cyclic dextran Sulfate, dextran sulfate, pullulan, magnesium chloride, sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, surfactant, preservative; Reagent R2 : 3‑morpholinopropanesulfonic acid (MOPS) buffer, cholesterol esterase (CEH), cholesterol oxidase (COD), peroxidase enzyme (POD), 4‑aminoantipyrine, flavin adenine di Nucleotide Disodium, Surfactant, Stabilizer, Preservative. The invention also provides the preparation method and application of the kit. The kit is a liquid kit with strong stability, strong anti-interference ability, high accuracy and good repeatability.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent measurement, in particular to a high-density lipoprotein cholesterol measurement kit, and also relates to a preparation method and application of the high-density lipoprotein cholesterol measurement kit. Background technique [0002] High-density lipoprotein cholesterol (High density lipoprotein cholesterol, HDL-C) is mainly synthesized in the liver. It is an anti-atherosclerotic lipoprotein, which can transport cholesterol from extrahepatic tissues to the liver for metabolism, and excrete it through bile. High-density lipoproteins can take up cholesterol from the cell membrane, catalyzed by lecithin cholesterol acyltransferase to form cholesterol esters, and then transfer the carried cholesterol esters to very low-density lipoproteins and low-density lipoproteins. The cholesterol content in high-density lipoprotein is relatively fixed, containing about 20% to 30% of the total choleste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/60C12Q1/44C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/44C12Q1/60C12Q2326/96
Inventor 刘安娜
Owner 中拓生物有限公司
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