A kit for measuring serum total antioxidant status

An antioxidant and kit technology, which is applied in material analysis by observing the impact on chemical indicators, measurement of color/spectral properties, and analysis by chemical reaction of materials, etc., can solve the problem of poor stability and anti-interference ability. Poor and other problems, to achieve the effect of improving stability, cheap price, and excellent performance indicators

Active Publication Date: 2021-03-30
中临健康科技产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current method for detecting the status of total antioxidants is mainly the colorimetric method in biochemical reagents. This method has the advantages of high specificity, simple and fast operation, accuracy and safety, automatic analysis, and low cost. The box has shortcomings such as poor stability and poor anti-interference ability. The present invention improves the shortcomings of the existing total antioxidant status kit to meet the requirements of clinical testing and chemical analysis.

Method used

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  • A kit for measuring serum total antioxidant status
  • A kit for measuring serum total antioxidant status
  • A kit for measuring serum total antioxidant status

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A conventional assay kit for total antioxidant status, which includes reagent R1 and reagent R2.

[0047] Reagent R1 contains the following components:

[0048] 3-morpholine propanesulfonic acid (MOPS) buffer 100 mmol / L;

[0049] Disodium edetate 0.1 mmol / L;

[0050] Sodium silicate 0.1 mmol / L;

[0051] Sodium pyrophosphate 0.1 mmol / L;

[0052] Triton x-100 0.1%;

[0053] proclin300 1ml / L;

[0054] Reagent R2 contains the following ingredients:

[0055] 3-morpholine propanesulfonic acid (MOPS) buffer 100mmol / L;

[0056] Potassium peroxodisulfate 0.5mmol / / L;

[0057] 2,2-azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS) 0.5mmol / L;

[0058] Potassium carbonate 0.1mmol / L;

[0059] Triton x-100 0.1%;

[0060] proclin300 1ml / L;

[0061] Wherein said percentage is volume ratio.

[0062] Reagent R1 and reagent R2 described in this example need to prepare 3-morpholine propanesulfonic acid buffer first, adjust the pH with hydrochloric acid or so...

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Abstract

The invention provides a total antioxidant state determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains a 3-morpholine propanesulfonic acid (MOPS) buffer solution, sodium silicate, sodium pyrophosphate, ethylenediamine tetraacetic acid disodium salt, a surfactant and a preservative; and the reagent R2 comprises the 3-morpholine propanesulfonic acid (MOPS) buffer solution, potassium peroxydisulfate, 2, 2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS), potassium carbonate, the surfactant and the preservative. The invention alsoprovides a preparation method and application of the kit. The kit is a liquid kit with strong stability, strong anti-interference capability, high accuracy and good repeatability.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent determination, in particular to a total antioxidant state determination kit, and also relates to the preparation method and application of the total antioxidant state determination kit. Background technique [0002] Reactive oxygen species (Reactive oxygen species, ROS) mainly include hydroxyl radicals, superoxide radicals and hydrogen peroxide. Active oxygen is produced during the normal physiological metabolism of cells or tissues, and some environmental factors such as ultraviolet radiation, γ-ray irradiation, smoking, environmental pollution, etc. can also induce the production of active oxygen. After the generation of reactive oxygen species, it can cause oxidative damage to intracellular lipids, proteins, and DNA, and induce oxidative stress, which in turn leads to various atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system damage. systemic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 刘安娜
Owner 中临健康科技产业有限公司
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