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Novel serum alpha fetoprotein variant 3 determination kit, preparation method and application thereof

A technology of alpha-fetoprotein and heterogenous body, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of expensive equipment, high price, inconvenient storage of reagents, etc., so as to improve the analytical sensitivity and accuracy, and enhance the specificity. The effect of high degree of stability and automation

Active Publication Date: 2021-01-22
中拓生物有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current method for detecting alpha-fetoprotein isoform 3 is mainly double-antibody sandwich immunochemiluminescence assay (ILMA), which has high accuracy and sensitivity, but the equipment is expensive, the reagents are inconvenient to store, and the price is high
Latex immunoturbidimetric method has the advantages of high specificity, simple and quick operation, accurate and safe, automatic analysis, and low cost. However, there is no AFP-L3 biochemical kit in China, only AFP kit

Method used

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  • Novel serum alpha fetoprotein variant 3 determination kit, preparation method and application thereof
  • Novel serum alpha fetoprotein variant 3 determination kit, preparation method and application thereof
  • Novel serum alpha fetoprotein variant 3 determination kit, preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0051] A conventional assay kit for alpha-fetoprotein heterosome 3, which includes reagent R1 and reagent R2.

[0052] Reagent R1 contains the following components:

[0053] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0054] NaCl 9g / L;

[0055] Bovine serum albumin (BSA) 10g / L;

[0056] Polyethylene glycol 6000 8g / L;

[0057] Trehalose 8g / L;

[0058] GLAURIN 1%;

[0059] proclin300 1 ml / L.

[0060] Reagent R1 has a pH value of 7.0.

[0061] Reagent R2 contains the following ingredients:

[0062] Piperazine-1,4-diethanesulfonic acid (PIPES) buffer solution 50mmol / L;

[0063] Goat anti-human AFP-L3 antibody coated latex particles 15mg / L;

[0064] Goat anti-human AFP-L3 antibody coated magnetic nanoparticles 15mg / L;

[0065] Bovine serum albumin (BSA) 5g / L;

[0066] Polyethylene glycol 6000 5g / L;

[0067] Trehalose 5g / L;

[0068] GLAURIN 1%;

[0069] proclin300 1ml / L.

[0070] Reagent R2 has a pH of 6.8.

[0071] Wherein said percentag...

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Abstract

The invention provides an alpha fetoprotein variant 3 (AFP-L3) determination kit. The kit contains a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components: a piperazine-1, 4-diethylsulfonic acid (PIPES) buffer solution, NaCl, a surfactant, a suspending aid and a preservative; and the reagent R2 is prepared from the piperazine-1, 4-diethylsulfonic acid (PIPES) buffersolution, goat anti-human AFP-L3 antibody coated latex particles, goat anti-human AFP-L3 antibody coated magnetic nanoparticles, the suspending aid, a stabilizer and the preservative. The invention also provides a preparation method and an application of the kit, and the kit can effectively replace a chemiluminescence immunoassay analyzer kit, is a novel liquid kit with strong stability, high sensitivity, good repeatability and low cost, and is convenient for clinical development and application of projects.

Description

technical field [0001] The present invention relates to the technical field of biochemical reagent determination, in particular to a kit for measuring alpha-fetoprotein heterogeneity 3, and also relates to a preparation method and application of the kit for determination of alpha-fetoprotein heterogeneity 3. Background technique [0002] Primary hepatocellular carcinoma (HCC) is one of the common malignant tumors in my country, with a high mortality rate, ranking third in the death order of malignant tumors after gastric cancer and esophageal cancer. my country is a big country with hepatitis B, and there are about 120 million cases of hepatitis B virus carriers. Every year, 130,000 patients die of liver cancer, accounting for 45% of the world's liver cancer deaths. Like other tumor screenings, the prevention and treatment of liver cancer mainly lies in the "three early", that is, early detection, early diagnosis and early treatment. Early liver cancer, if treated scientifi...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/543G01N33/531
CPCG01N33/531G01N33/54326G01N33/54346G01N33/57438G01N33/57476
Inventor 刘安娜张刘云
Owner 中拓生物有限公司
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