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Serum alpha 1-acid glycoprotein measurement kit

An acid glycoprotein, bovine serum albumin technology, applied in biological testing, measuring devices, material testing products, etc., can solve problems such as poor stability and low sensitivity, and achieve improved analytical sensitivity, stability, and dispersion. Effects of sex and biocompatibility and targeting

Inactive Publication Date: 2019-05-10
中拓生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the α1-acid glycoprotein assay kit with good linear range and high sensitivity in the market currently has samples limited to urine and cannot detect α1-acid glycoprotein in human serum (such as the reagents disclosed in CN 105403712 A and CN 105628942 A box), and the test kit used to detect α1-acid glycoprotein (such as the kit disclosed in CN 103076456 A) in human serum has the problems of poor stability and low sensitivity. The technical problem to be solved by the present invention is to provide A α1-acid glycoprotein assay kit with high sensitivity, good linear range and strong stability, which can detect serum samples in batches

Method used

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  • Serum alpha 1-acid glycoprotein measurement kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053]An α1-acid glycoprotein immunoturbidimetric detection kit, which includes reagent R1, reagent R2, and calibrator.

[0054] The reagent R1 is composed of:

[0055] Phosphate buffer 18mmol / L

[0056] Sodium chloride 110mmol / L

[0057] Magnetic nanoparticles coated with polyethylene glycol 1%

[0058] pH 7.5

[0059] Reagent R2 consists of:

[0060] Phosphate buffer 15mmol / L

[0061] Goat anti-human α1-acid glycoprotein antibody 32mg / ml

[0062] Triton 100 1 g / L

[0063] Bovine serum albumin 1 g / L

[0064] Kason 1 g / L

[0065] pH 6.0

[0066] The calibrator consisted of:

[0067] Phosphate buffer 25mmol / L

[0068] α1-acid glycoprotein antigen 13mg / ml

[0069] Bovine serum albumin 1g / L

[0070] Mannitol 1g / L

[0071] Sodium azide 1g / L

[0072] pH 5.5

[0073] The preparation method of the above-mentioned α1-acid glycoprotein assay reagent comprises the following steps:

[0074] (a) Preparation of polyethylene glycol-coated magnetic nanoparticles: Weigh 9 g o...

Embodiment 2

[0079] Reagent R1 consists of:

[0080] Phosphate buffer 20mmol / L

[0081] Sodium chloride 110mmol / L

[0082] Magnetic nanoparticles coated with polyethylene glycol 1%

[0083] pH 7.5

[0084] Reagent R2 consists of:

[0085] Phosphate buffer 15mmol / L

[0086] Goat anti-human α1-acid glycoprotein antibody 30mg / ml

[0087] Tween-20 1 g / L

[0088] Bovine serum albumin 1 g / L

[0089] proclin300 1 g / L

[0090] pH 6.0

[0091] The calibrator consisted of:

[0092] Phosphate buffer 25mmol / L

[0093] α1-acid glycoprotein antigen 13mg / ml

[0094] Bovine serum albumin 1g / L

[0095] Mannitol 1g / L

[0096] Sodium azide 1g / L

[0097] pH 5.5

[0098] The preparation method is the same as in Example 1.

Embodiment 3

[0100] Reagent R1 consists of:

[0101] Phosphate buffer 20mmol / L

[0102] Sodium chloride 110mmol / L

[0103] Magnetic nanoparticles coated with polyethylene glycol 1.5%

[0104] pH 7.5

[0105] Reagent R2 consists of:

[0106] Phosphate buffer 15mmol / L

[0107] Goat anti-human α1-acid glycoprotein antibody 32mg / ml

[0108] Triton X-100 1 g / L

[0109] Bovine serum albumin 1 g / L

[0110] proclin300 1 g / L

[0111] pH 6.0

[0112] The calibrator consisted of:

[0113] Phosphate buffer 25mmol / L

[0114] α1-acid glycoprotein antigen 13mg / ml

[0115] Bovine serum albumin 1g / L

[0116] Mannitol 1g / L

[0117] Sodium azide 1g / L

[0118] pH 5.5

[0119] The preparation method is the same as in Example 1.

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Abstract

The invention discloses a serum alpha 1-acid glycoprotein measurement kit, comprising a reagent R1, a reagent R2 and a calibration product, wherein the reagent R1 is composed of a buffer solution, sodium chloride and polyethylene glycol coated magnetic nano-particles; the reagent R2 is composed of a buffer solution, a goat anti-human alpha 1-acid glycoprotein antibody, a surfactant, a stabilizer and a preservative; the calibration product is composed of a buffer solution, an alpha 1-acid glycoprotein antigen, bovine serum albumin, mannitol and sodium azide. The invention further discloses an application of the kit. The alpha 1-acid glycoprotein is a liquid double-reagent, which can be directly used by opening the bottle without reconstitution. By adding the polyethylene glycol coated magnetic nano-particles to the reagent R1, the stability of the magnetic nano-particles is improved, and the antibody forms agglomeration on the magnetic nano-particles, thereby greatly improving the analytical sensitivity of the reagent. By adding the bovine serum albumin and glycerol to the reagent R2, the stability of the reagent is improved.

Description

technical field [0001] The invention relates to the field of medical immunological in vitro diagnostic reagents, in particular to an α1-acid glycoprotein assay kit, and also relates to a preparation method and application of the α1-acid glycoprotein assay kit. Background technique [0002] α1-acid glycoprotein (α1-acidglycoprotein, AAG, earlier known as whey mucinoid) has a molecular weight of nearly 40,000, contains about 45% sugar, and has a pI of 2.7-3.5, including hexose, hexosamine and Sialic acid is the glycoprotein with the highest sugar content and the strongest acidity in human blood. Because the AAG gene structure and amino acid sequence are similar to those of retinal binding protein and β1-microglobulin, it is considered to be a member of the lipocalin superfamily. [0003] α1-acid glycoprotein is the main acute phase reaction protein. When stimulated by inflammation, the concentration of AAG can increase rapidly, and it is a sensitive marker of inflammation in t...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/531G01N33/537G01N33/577
Inventor 解苇生王飞刘安娜隗勇
Owner 中拓生物有限公司
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