52 results about "Adenine dinucleotides" patented technology

Object: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors.

Method for Achieving the Object: A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.

The invention discloses a recombinant human NADH (nicotinamide-adenine dinucleotid) dehydrogenase subunit-4 gene and a constructing method of an expression vector thereof. The nucleotide sequence of the gene is shown in SEQ ID NO:1, and the size of the nucleotide sequence is 2889 bp. The constructing steps of adeno-associate virus vectors are as follows: firstly constructing a recombinant adeno-associated virus vector containing the human NADH dehydrogenase subunit-4 gene, and then coating, infecting, purifying, concentrating and identifying the recombinant adeno-associated virus. According to the method, the recombinant adeno-associated virus vector with the recombinant human NADH dehydrogenase subunit-4 gene can be quickly and simply constructed, and is packaged to obtain the adeno-associated virus vector with complex defects. The gene can be used for gene therapy of LHON (leber's hereditary optic neuropathy).

The invention relates generally to the field of industrial microbiology and alcohol production. More specifically, the invention relates to the use of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) to improve butanol production. Butanol production can be improved by providing sufficient amounts of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) in the production media.

Compositions for addressing one or more of the effects of aging are described. The compositions comprise a first component comprising repair system activator(s) such as nicotinamide adenine dinucleotide (NAD+), nicotinamide mononucleotide (NMN), nicotinamide riboside (NR), nicotinic acid adenine mononucleotide (NaMN), nicotinic acid adenine dinucleotide (NaAD), nicotinic acid riboside (NAR), 1-methylnicotinamide (MNM), cyclic adenosine monophosphate (cAMP) and combinations thereof; a second component comprising methyl donor(s), such as S-5'-adenosyl-L-methionine (SAM), methionine, betaine, choline, folate, vitamin B12, or combinations thereof; and a third component comprising antioxidant defense activators, such as H2O2, N2S, NaSH, Na2S, and several others, including combinations thereof.Methods of administering the disclosed compositions or separate formulations of repair system activator, methyl donors, and antioxidant defense activators are also disclosed.

The invention relates to a palladium nanoparticle/carbon nanofiber compound, a preparation method and an application thereof in electrocatalysis. The compound is directly prepared by a one-step electrospinning method. An electrode modified by the compound is used for direct eectrochemical detection on hydrogen peroxide, beta-nicotinamide adenine dinucleotide, dopamine, ascorbic acid and lithic acid. The electrode modified by the compound has the linear range from 0.2 micrometer to 20 millimetres and the detection limit of 0.2 micrometer when being used for the detection on the hydrogen peroxide and has the linear range of 0.2-716.6 micrometers and the detection limit of 0.2 micrometer when being used for the detection on the beta- nicotinamide adenine dinucleotide; and the peak-peak potential differences between the ascorbic acid and the dopamine, the dopamine and the lithic acid as well as the ascorbic acid and the lithic acid are respectively 244mV, 148 mV and 392 mV, thereby showingthat the modified electrode can be used for simultaneous eectrochemical detection of three substances. The compound can be used in the fields of catalysis, fuel cells and sensing.

It is intended to highly efficiently produce a large amount of novel FAD-GDH capable of more accurately measuring a glucose level. Provided is a transformant in which a DNA encoding a flavin adenine dinucleotide-binding glucose dehydrogenase selected from the group consisting of (a) a DNA encoding the amino acid sequence of SEQ ID NO: 20; (b) a DNA consisting of the base sequence of SEQ ID NO: 19; (c) a DNA having a base sequence homologous to the base sequence of SEQ ID NO: 19 and encoding a protein having a flavin adenine dinucleotide-binding glucose dehydrogenase activity; (d) a DNA encoding the amino acid sequence of SEQ ID NO: 34; (e) a DNA consisting of the base sequence of SEQ ID NO: 33; and (f) a DNA having a base sequence homologous to the base sequence of SEQ ID NO: 33 and encoding a protein having a flavin adenine dinucleotide-binding glucose dehydrogenase activity has been introduced. Further, a method for producing a flavin adenine dinucleotide-binding glucose dehydrogenase using the transformant is provided.

The invention discloses a construction method of a saccharomyces cerevisiae strain with a high yield of isobutanol and relates to preparation of microorganism by a recombinant DNA (Deoxyribonucleic Acid) technology. According to the method, the saccharomyces cerevisiae strain with the high yield of isobutanol is constructed by reforming saccharomyces cerevisiae cells by using molecular biology and the gene recombination technology. The method comprises the following steps: S1, constructing plasmids of over-expressing ILV2 (Induced Leukemia Virus), ILV3, ILV5 and ZWF1 genes of saccharomyces cerevisiae; and S2, constructing a saccharomyces cerevisiae strain HBGDAL-103 with the high yield of isobutanol. The method disclosed by the invention overcomes the deficiency that further improvement of the yield of isobutanol is restricted by factors such as imbalance of a coenzyme NADPH/NADP<+> (Reduced Form of Nicotinamide-Adenine Dinucleotide Phosphate)/(Nicotinamide-Adenine Dinucleotide Phosphate<+>) from pyruvic acid to 2-one isovaleric acid due to over-expressed ILV2, ILV3 and ILV5 in the saccharomyces cerevisiae cells of yielding isobutanol in the prior art.

The invention provides a carbon dioxide measurement kit. The carbon dioxide measurement kit mainly comprises the following components: 50 to 200mM of a buffer solution, 10 to 50% of a stabilizer, 0.1 to 5% of a surface active agent, 0.1 to 5g/L of a preservative, 1 to 20mM of a reaction accelerator, 2 to 10mM of 3-acetylpyridine adenine dinucleotide (reduction type), 5 to 50g/L of phosphoenolpyruvic acid, 50 to 500U/L of phosphoenolpyruvate carboxylase, and 1 to 3KU/L of malate dehydrogenase; a recycling system of 3-acetylpyridine adenine dinucleotide (reduction type) comprises (1) 2 to 20g/L of glucose substrate, (2) 100 to 2,000U/L of corresponding glucose dehydrogenase, and (3) 1 to 5mM of 3-acetylpyridine adenine dinucleotide. The carbon dioxide measurement kit is convenient to use and simple to operate; the components participating in coupling reaction are extra added, so that no pollution caused by endogenous and allogenic substances are introduced; the rate of the recycling system is below the main reaction rate, thus the main reaction cannot be interfered; the carbon dioxide measurement kit is high in stability and can be used for a long time.

The invention belongs to the technical field of analytical chemistry and relates to a detection method and application of PARP [poly(adenosine diphosphate-ribose) polymerase]. The detection method mainly includes: modifying a single-stranded DNA (c-kit-1) capable of specifically binding with the PARP on the surface of a gold electrode in a classical mercapto self-assembly mode and enabling c-kit-1 to form a tetramer configuration through treatment; after the tetramer configuration is incubated with the PARP, adding a specific catalytic substrate, namely NAD (nicotinamide ademinedinucleotide) of the PARP, to enable the PARP to self-catalyze to produce PAR with high negative charges; using the negative charges of the PAR for adsorbing electrical signal molecules RuHex with positive charges, quantifying the RuHex adsorbed on the surface of the electrode through an electroanalysis method, and drawing a standard curve according to a relation between electrochemical signals and PARP concentration so as to achieve sensitive PARP detection by measuring the electrochemical signals of to-be-detected samples and calculating. The detection method is high in repeatability and sensitivity and can be applied to detection of the PARP and an inhibitor 3-AB (3-aminobenzamide) thereof.

The invention discloses a cyclophorase detection method for quantitative detection of thio-nicotinamide-adenine dinucleotide (Thio-NAD). The cyclophorase detection method comprises the following steps of: preparing Tris-HCl buffer solution with pH of 8.5 to 10.50, wherein the Tris-HCl buffer solution includes a certain amount of coenzyme I (NADH), a sodium deoxycholate solution, 3Alpha-hydroxysteroiddehydrogenase (3Alpha-HSD) and trehalose, and adding a Thio-NAD-containing sample to be tested; dehydrogenizing and oxidizing sodium cholate or sodium deoxycholate by 3Alpha-HSD to form 3Alpha-ketosteroid; meanwhile, reducing Thio-NAD into reduced-type thio-nicotinamide-adenine dinucleotide (Thio-NADH); re-reducing 3Alpha-ketosteroid into sodium cholate or derivatives thereof by 3Alpha-HSD in the presence of NADH in the solution; meanwhile, dehydrogenating and oxidizing the NADH into NAD<+>, wherein the Thio NADH generated in the reaction has maximum absorption at a 405 nm place; and computing content of THE Thio-NADH of the sample to be tested by measuring optical density of the solution under a specific wavelength after reacting at 37 DEG C for some time. The cyclophorase detection method, provided by the invention, has the advantages of environmental friendliness, and stable test reagent, and is particularly suitable for bulk detection of Thio-NAD.

A sensor for detecting lactate dehydrogenase, used for detecting a lactate dehydrogenase content of a biological sample, comprising a substrate, an electrode group, a hydrophobic insulating layer, a cover and a reaction drug membrane. The electrode group includes a working electrode and a reference electrode and is located on the substrate, the hydrophobic insulating layer is arranged on the substrate and includes a gap, the cover covers the gap and is connected to the substrate A sample channel is formed between them to accommodate the reaction drug film, the reaction drug film is selected from the group consisting of lactic acid, nicotinamide adenine dinucleotide, diaphorase and potassium ferricyanide. The reaction drug film is in contact with the lactate dehydrogenase in the biological sample to generate a bioelectrochemical reaction, so that the electrode group undergoes an electrical signal change reflecting the content of the lactate dehydrogenase.

The invention discloses a method for improving bacillus glycerin metabolism to increase yield of poly gamma-glutamic acid by enhancing synthesis of flavin adenine dinucleotide (FAD). Three FAD enhanced synthetic strains are respectively built by enhancing expression of three key genes ribE, ribF and ribH in bacillus in a FAD synthesis path. In different liquid fermentation conditions, overexpression of ribE, ribF and ribH enables glycerin metabolic capacity to be improved by at least 22.02%, 20.62% and 23.92% respectively when compared with that of a comparison strain, and poly gamma-glutamicacid yield of these recombinant strains are at least 1.20, 1.18 and 1.27 times of that of the comparison strain. It shows that enhancing of FAD synthesis can remarkably improve bacillus glycerin metabolism and increase synthesis of poly gamma-glutamic acid. The method can improve glycerin utilization to enhance synthesis of a target produce and has wide application value in the aspect of utilizingglycerin as a raw material for microbial fermentation to synthesize a bio-based compound, and a new strategy is provided for microorganisms to efficiently utilize glycerin to synthesize bio-based chemicals.

The invention provides a method for preparing nucleosides of nicotinic acid or derivatives thereof, nicotinic acid adenine dinucleotide and nicotinic acid mononucleotide, an enzyme composition and application. The method for preparing the nucleosides of the nicotinic acid or the derivatives thereof comprises the step of carrying out reaction by using 5'-nucleotidase and mononucleotide of the nicotinic acid or the derivatives thereof or salt of the mononucleotide as a substrate, wherein the amino acid sequence of the 5'-nucleotidase is shown as SEQ ID NO.1. Compared with a chemical synthesis method, the method is safer and more reliable, and is more environment-friendly because the use of an organic solvent can be avoided. Nicotinamide nucleoside can be more effectively produced so as to meet increasing demands.

The invention discloses a transhydrogenase-1 activity determination kit and a use method thereof. The kit comprises a mixed solution of a Tris-HCl buffer solution, sucrose and EDTA, a Tris-HCl buffersolution, NADH and AcPyADP. The method is based on the detection principle of replacing the NADP<+> with a synthetic substrate 3-acetylpyridine adenine dinucleotide phosphate (APADP<+>) and calculating TH-1 activity through measuring a light absorption increasing speed at 375nm of APADPH generated by the reduction of APADP<+> catalyzed by TH-1, can effectively eliminate the interference of NAD andhas high detection specificity. Through a simple work liquid, only through adding a sample and a work liquid into a microcuvette or a 96-well plate, the determination is finished and detection is convenient. The kit can effectively isolate cytoplasmic proteins and mitochondrial proteins and accurately determine TH-1 located in the mitochondrial inner membrane.

The invention discloses a method for continuously preparing a 2-benzyl isoindolinone compound by adopting a microchannel reactor, which comprises the following steps: (1) respectively and simultaneously pumping a mixed solution containing 1,2-Benzenedimethanol, alcohol oxidase, flavin adenine dinucleotide and hydrogen peroxide reductase and air into a microchannel reaction device; carrying out a first reaction in a first microreactor containing nano MnO2; and (2) respectively and simultaneously pumping the first reaction effluent and a benzylamine compound solution into a second microreactor, and carrying out a second reaction to obtain the 2-benzyl isoindolinone compound as shown in a formula IV. The method has the advantages of short reaction time, high reaction conversion rate, few side reactions, small toxicity and pollution, low production cost, good product quality and the like, does not use noble metal catalysis in the reaction process, is green, environment-friendly, energy-saving and efficient, is suitable for popularization and application, and solves the problems of long reaction time, harsh reaction conditions and need of noble metal catalytic catalyst in the prior art.

The invention belongs to the technical field of agricultural weeding chemicals, and particularly relates to a novel weedicide which is mainly applicable to weeding of sugarcane, corn and sorghum fields. The novel weedicide is characterized by comprising the following active ingredients in percentage by weight: 20-80% of simazine, 20-80% of atrazine, 3-15% of auxiliaries, and the balance of fillers. The novel weedicide belongs to uptake and translocation type weedicide, and inhibits the photophosphorylation action in a plant body through the uptake and translocation of stem leaves and roots of plants, the processes of NADP (nicotinamide - adenine dinucleotide phosphate) reduction and CO2 fixation and the like, damages the photosynthesis of plants, and influences the absorption of nutrients in weeds so as to stop the growth of plants. According to the novel weedicide, two ingredients have synergistic interaction, the weedicide controlling spectrum can be effectively extended, various weeds can be prevented through once application, the application amount of the weedicide can be greatly decreased, the cost can be lowered and the adverse effect on the environment can be reduced, the drug resistance of the weeds can be postponed, and the defects that the drug resistance of the weeds due to long-time use of one weedicide is increased, weedicide effect is decreased, and the like can be overcome.

The invention discloses a chemical synthesis method of 3-acetylpyridine adenine dinucleotide, comprising the following steps: (1) D-ribose is used as a starting material to prepare monophosphate-3-acetylpyridine-alpha-D-nucleoside; (2) adenosine is used as a main raw material to prepare morpholine adenosine monophosphate; and (3) a docking reaction between morpholine adenosine monophosphate and monophosphate-3-acetylpyridine-alpha-D-nucleoside is carried out to prepare 3-acetylpyridine adenine dinucleotide. According to the chemical synthesis method, a commercial industrial product D-ribose is used as the starting raw material; any enzyme is not used during the preparation process of an intermediate; price of raw materials is low and the raw materials are easy to purchase; and production cost of the product is reduced greatly. In addition, by the chemical synthesis method, yield of the product is high, and total yield of the reaction is 4.2% and is remarkably higher than product yield of a present biosynthesis method.

The invention discloses a nicotinamide ribose derivative cosmetic raw material and an application thereof in cosmetics. The nicotinamide ribose derivative cosmetic raw material comprises the followingcomponents in parts by weight: 15 to 20 parts of nicotinamide ribose chloride; 10 to 14 parts of dihydronicotinamide ribose, 10 to 12 parts of beta-nicotinamide mononucleotide, 4 to 7 parts of NAD4,3 to 5 parts of NADH, 4 to 6 parts of oxidized nicotinamide adenine dinucleotide sodium salt, 4 to 6 parts of thiooxidized nicotinamide adenine dinucleotide and 4 to 6 parts of oxidized nicotinamide adenine dinucleotide lithium salt, and the nicotinamide ribose derivative cosmetic raw material relates to the technical field of cosmetics. The invention discloses the nicotinamide ribose derivative cosmetic raw material and the application thereof in cosmetics, the nicotinamide ribose and derivatives thereof can improve the mitochondrial function in cells; mitochondrial function decline is closely related to human aging, functions of sirtuin are activated, mitochondrial activity is improved, and cell damage caused by free radicals is prevented, so that the effect of delaying cell aging is achieved, functions of epidermal cells and other cells in a human body are improved, skin health can be maintained, and skin aging is delayed.

The invention particularly relates to a fusion enzyme and application of the fusion enzyme in a paper-based biosensor. Glucose dehydrogenase (GDH) taking flavin adenine dinucleotide (FAD) as a coenzyme has good stability, is developed into a portable glucose sensor and has a good application prospect. The invention provides a GDH-linker-CBM fusion enzyme constructed by a flavin adenine dinucleotide (FAD) dependent glucose dehydrogenase (GDH) gene and a carbohydrate binding module (CBM) gene, and the GDH-linker-CBM fusion enzyme is prepared into a paper-based sensor through the modification effect of the carbohydrate binding module and a connecting peptide. The enzyme activity, the detection sensitivity and the anti-interference capability of the paper-based sensor are effectively improved, and a good market development prospect is realized.