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Sensor used for detecting lactic dehydrogenase

A lactate dehydrogenase and sensor technology, applied in the field of sensors for detecting lactate dehydrogenase, can solve problems such as inaccurate detection results, and achieve the effect of wide application range

Inactive Publication Date: 2016-10-05
EUMED BIOTECH
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  • Abstract
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Problems solved by technology

[0005] The main purpose of the present invention is to solve the problem of inaccurate detection results of existing lactate dehydrogenase sensors

Method used

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  • Sensor used for detecting lactic dehydrogenase
  • Sensor used for detecting lactic dehydrogenase
  • Sensor used for detecting lactic dehydrogenase

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Embodiment Construction

[0016] The detailed description and technical content related to the present invention are described as follows in conjunction with the accompanying drawings:

[0017] Please refer to figure 1 , figure 2 , image 3 , Figure 4 as well as Figure 5 shown, respectively, are a first appearance perspective view, a first structure exploded view, a second appearance perspective view, a second structure exploded view, and a top view of an embodiment of the present invention. As shown in the figure, the present invention is a sensor for detecting lactate dehydrogenase, which can be applied to detect the content of a lactate dehydrogenase in a biological sample. The sensor includes: a substrate ( 10), an electrode group (20), a hydrophobic insulating layer (30) and a cover body (40). The substrate (10) in this embodiment is elongated and has a periphery (11), the periphery (11) includes two long sides (111) and two short sides (112), the long sides (111) ) are parallel to each o...

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Abstract

A sensor for detecting lactate dehydrogenase, used for detecting a lactate dehydrogenase content of a biological sample, comprising a substrate, an electrode group, a hydrophobic insulating layer, a cover and a reaction drug membrane. The electrode group includes a working electrode and a reference electrode and is located on the substrate, the hydrophobic insulating layer is arranged on the substrate and includes a gap, the cover covers the gap and is connected to the substrate A sample channel is formed between them to accommodate the reaction drug film, the reaction drug film is selected from the group consisting of lactic acid, nicotinamide adenine dinucleotide, diaphorase and potassium ferricyanide. The reaction drug film is in contact with the lactate dehydrogenase in the biological sample to generate a bioelectrochemical reaction, so that the electrode group undergoes an electrical signal change reflecting the content of the lactate dehydrogenase.

Description

technical field [0001] The present invention relates to a sensor for detecting protease, in particular to a sensor for detecting lactate dehydrogenase Background technique [0002] Lactate dehydrogenase is an enzyme that catalyzes the mutual conversion of lactate and pyruvate. It exists in all animal tissues. It mainly exists in five forms in animals, namely LDH1, LDH2, LDH3, LDH4 and LDH5. LDH1 and LDH2 are more abundant in human myocardium, kidney and erythrocytes, and LDH4 and LDH5 are more abundant in skeletal muscle and liver. However, LDH3 is more abundant in tissues such as lung, spleen, pancreas, thyroid, adrenal gland and lymph node, so its tissue specificity can be used as a preliminary indicator for disease diagnosis. According to research, when humans or animals are infected, the level of lactate dehydrogenase in their bodies will increase, for example: when humans suffer from hepatitis, acute liver cell damage and skeletal muscle damage, LDH5 will increase; LD...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 沈燕元彭啟扬陈择铭
Owner EUMED BIOTECH
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