The invention discloses a tumor isocenter multi-angle non-planar percutaneous puncture method belonging to a novel method that a doctor carries out tumor percutaneous puncture on a tumor patient under the guidance of CT in the technical field of medical treatment, and solving the problems of high dependence on experiences and techniques of an operator, easy generation of larger puncture errors, long occupation time of puncture, large difficulty in puncturing smaller tumors or deeper tumors in early stage, and low success rate in the tumor percutaneous puncture technology. The technical scheme is as follows: the tumor isocenter multi-angle non-planar percutaneous puncture method comprises the steps of sharing an isocenter by a center point of a semi-circular piercer and a center point of a tumor through coordinate system transfer, and driving a puncture needle for realizing multi-angle non-planar puncture through sliding of an arrow rest and rotating of the piercer. The tumor isocenter multi-angle non-planar percutaneous puncture method has the advantages that the problems of high dependence on experiences and techniques of the operator, easy generation of larger puncture errors, long occupation time of puncture, large difficulty in puncturing smaller tumors or deeper tumors in early stage, and low success rate in the tumor percutaneous puncture technology are solved, vital organs, puncture taboo organs and taboo focuses can be effectively avoided, the tumor percutaneous puncture error can be shortened to 2mm under the guidance of the CT, and the highest tumor percutaneous puncture precision at home and abroad is achieved.