Three-component biological glue as well as preparation and application thereof

A three-component, biological technology, used in tissue regeneration, drug delivery, medical science, etc., can solve the problems of lack of clinical treatment donors, inability to anastomose grafts, etc., to increase tissue adhesion, enhance mechanical strength, and reduce cost. The effect of glue time

Inactive Publication Date: 2017-09-12
HAINING ZHULUOJI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a three-component bioglue for soft tissue and vital organ defect repair and its preparation and application, which solves the problems of lack of donors and inability of grafts to match defects in current clinical treatment.

Method used

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  • Three-component biological glue as well as preparation and application thereof
  • Three-component biological glue as well as preparation and application thereof
  • Three-component biological glue as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Aldehydated natural polysaccharide: Dissolve 2g of carboxymethyl cellulose in 100ml of deionized water to prepare a 2% mass concentration solution. After it is completely dissolved, add 1g of sodium periodate and react for 1 hour at 20°C in the dark, then use 1.8ml of ethylene glycol terminates the reaction. Pour the reaction solution into a dialysis bag (permeate molecular weight of 8000-14000Da), use deionized water as the dialysate, dialyze at 15°C for 2 days, then store at -20°C for 0.5 hours, and then store at -80°C for 5 hours , And then freeze-dried at -50°C and 1 Pa for 24 hours to obtain aldehyde carboxymethyl cellulose with an aldehyde oxidation rate of 40%.

[0029] 2) Acrylic anhydride grafted natural polymer: Dissolve 1mmol of hyaluronic acid in 100ml of deionized water, then add 1mmol of hydrazine, after complete mixing, add 1mmol of 1-(3-dimethylaminopropyl)-3-ethyl A mixture of carbodiimide hydrochloride (EDC) and 1 mmol of N-hydroxysuccinimide (NHS) wa...

Embodiment 2

[0033] 1) Aldehydlated natural polysaccharide: Dissolve 10g dextran (molecular weight 70,000) in 100ml deionized water to prepare a solution with a mass concentration of 10%. After it is completely dissolved, add 10g sodium perchlorate and react for 3h at 25°C in the dark. The reaction was then terminated with 9 ml of ethylene glycol. Pour the reaction solution into a dialysis bag (permeate molecular weight of 8000-14000Da), use deionized water as the dialysate, dialyze at 25°C for 7 days, then take the retentate and store it at -20°C for 3 hours, then at -80°C Placed for 12 hours, then freeze-dried at -50°C and 99 Pa for 96 hours to obtain an aldehyde group dextran with an aldehyde group oxidation rate of 50%.

[0034] 2) Amino-modified natural biopolymer: Dissolve 1 mmol (purchased from Sigma) gelatin in 100 ml deionized water, and then add 30 mmol adipic acid dihydrazide. After complete mixing, add 5mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and...

Embodiment 3

[0038] 1) Aldehydated hyaluronic acid: Dissolve 2g of hyaluronic acid in 100ml of deionized water to prepare a solution with a mass concentration of 2%. After it is completely dissolved, add 2g of potassium periodate and react for 12h at 25°C in the dark, and then use 1.8 ml ethylene glycol terminates the reaction. Pour the reaction solution into a dialysis bag (permeate molecular weight of 8000-14000Da), use deionized water as the dialysate, dialyze at 15°C for 4 days, then take the retentate and store at -20°C for 1.5 hours, and then at -80°C Placed for 10 hours, then freeze-dried at -50°C and 50 Pa for 48 hours to obtain aldehyde-based hyaluronic acid with an aldehyde group oxidation rate of 42%.

[0039] 2) Acrylic anhydride grafted sodium alginate: Dissolve 1 mmol sodium alginate in 100 ml deionized water, and then add 15 mmol adipic acid dihydrazide. After mixing completely, add 3mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 3mmol of N-hydro...

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Abstract

The invention discloses three-component biological glue as well as preparation and application thereof. The three-component biological glue is prepared by mixing 1 to 20 percent by mass of aldehyde natural polysaccharide solutions, 1 to 20 percent by mass of amino modified natural biological macromolecular solutions and 1 to 15 percent by mass of acrylic anhydride grafted natural macromolecular solutions according to a volume ratio of 1:0.1-2:0.1-2. The tissue adhesiveness, the glue-forming time and the mechanical strength of the three-component biological glue for repairing defects of soft tissues and vital organ can be controlled by aldehyde content of aldehyde natural polysaccharide, the grafting density of amino in amino-modified natural biological macromolecules, the grafting density of the acrylic anhydride in acrylic anhydride modified natural macromolecules, the concentration of the aldehyde natural polysaccharide solutions, the concentration of the amino-modified natural biological macromolecular solutions and the concentration of acrylic anhydride grafted natural macromolecular solutions.

Description

(1) Technical field [0001] The invention relates to a three-component biological glue for soft tissue and important organ defect repair, and its preparation and application, which can be used in the field of tissue engineering. (2) Background technology [0002] Soft tissues include human skin, subcutaneous tissue, muscles, tendons, ligaments, joint capsules, synovial sacs, nerves, blood vessels, etc. Soft tissues are often traumatized or damaged due to external forces. Small traumas can be repaired spontaneously. For large traumas and defects, the body's self-repairing ability is very limited. Important organs, such as the liver and heart, can also be damaged due to congenital diseases or external forces. Similarly, the body's ability to repair itself is very limited. [0003] For major trauma and vital organ defects, there is also a lack of corresponding repair methods in clinical treatment. The current mainstream treatment is autologous tissue transplantation and surgical sutu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/52A61L27/60C08B11/12C08B11/20C08B37/04C08B37/08C08B37/02C08F251/00C08F251/02C08F220/32C08F220/08
CPCA61L27/26A61L27/52A61L27/60A61L2400/06A61L2430/20A61L2430/28A61L2430/34C08B11/12C08B11/20C08B37/0009C08B37/0024C08B37/0069C08B37/0072C08B37/0084C08F251/00C08F251/02C08F220/08C08F220/325
Inventor 周飞飞洪逸章淑芳欧阳宏伟
Owner HAINING ZHULUOJI BIOTECHNOLOGY CO LTD
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