Composition for reducing uric acid and relieving gout and preparation method thereof

Abstract

The invention provides a composition for reducing uric acid and relieving gout and a preparation method thereof, and relates to the technical field of products for reducing uric acid and relieving gout. The composition comprises the following raw materials: 18-24 parts of phellinus igniarius, 9-13 parts of sunflower disc small molecule peptide extract, 3-7 parts of corn stigma, 4-6 parts of poria cocos, 2-5 parts of pawpaw, 3-6 parts of liquorice, 2-5 parts of astragalus extract and 3-6 parts of lettuce extract. The preparation method of the sunflower disc small-molecule peptide extract comprises the following steps: carrying out enzymolysis on sunflower disc powder twice, carrying out membrane filtration to obtain a sunflower disc small-molecule peptide solution, reacting the sunflower disc small-molecule peptide solution with chitosan oligosaccharide and transglutaminase, and carrying out freeze drying to obtain the sunflower disc small-molecule peptide extract. Through the proper raw material composition and proportion, the metabolic function can be effectively regulated to promote excretion of uric acid, so that the content of xanthine oxidase and adenosine deaminase in the liver is obviously reduced, the uric acid level is effectively reduced, the intestinal probiotic structure can be stabilized, the immunity is enhanced, the kidney is protected, and the good effects of reducing uric acid and relieving gout are achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of uric acid-lowering and gout-relieving products, in particular to a uric acid-lowering and gout-relieving composition and a preparation method thereof. BACKGROUND

[0002] Gout is a metabolic disease caused by abnormal metabolism of purine substances, leading to increased synthesis of blood uric acid or (and) decreased uric acid excretion, and deposition of sodium urate crystals in joints and other connective tissues. In recent years, with the improvement of people's living standards, excessive intake of high-purine food in daily diet leads to metabolic abnormalities, which is prone to cause gout. Patients with gout usually show joint redness, fever, and unbearable pain. At present, the main western medicine for treating gout is colchicine, non-steroidal anti-inflammatory drugs, corticosteroids and selective cyclooxygenase-2 inhibitors. These drugs have significant effects in the short term, but long-term use can cause diarrhea, abdominal pain, nausea, kidney failure and other adverse reactions, and can easily lead to disease recurrence after drug withdrawal, with poor patient compliance. Compared with western medicine, the raw materials derived from traditional Chinese medicine have higher safety, and can act on multiple links from multiple channels and multiple targets, which can make up for the defects of adverse effects of western medicine treatment, and can effectively control the occurrence and development. At present, various drugs and edible products for treating gout have appeared on the market, but the effect of lowering uric acid and relieving gout still needs to be improved. SUMMARY

[0003] In view of this, the present application provides a uric acid-lowering and gout-relieving composition and a preparation method thereof.

[0004] The technical scheme of the present application is as follows: A uric acid-lowering and gout-relieving composition comprises the following raw materials by weight: 18-24 parts of Phellinus baumii, 9-13 parts of sunflower disc small molecule peptide extract, 3-7 parts of corn silk, 4-6 parts of Poria cocos, 2-5 parts of papaya, 3-6 parts of licorice, 2-5 parts of radix astragali extract, and 3-6 parts of lettuce extract. The preparation method of the sunflower disc small molecule peptide extract is as follows: the sunflower disc is dried and ground to obtain sunflower disc powder, water and compound enzyme A are added to the sunflower disc powder for primary enzymolysis, then filtration is performed, the primary enzymolysis liquid is taken and compound enzyme B is added for secondary enzymolysis, the enzyme is inactivated, filtration is performed again, the secondary enzymolysis liquid is taken and subjected to microfiltration membrane and ultrafiltration membrane to obtain a polypeptide solution with a molecular weight less than 1000 Da, then chitooligosaccharide and transglutaminase are added to the sunflower disc small molecule peptide solution, stirring is performed, the enzyme is inactivated, and freeze-drying is performed to obtain the sunflower disc small molecule peptide extract.

[0005] Further, the complex enzyme A includes cellulase and β-glucanase with a mass ratio of 2-3:1-2; the complex enzyme B includes trypsin and alkaline protease with a mass ratio of 2-4:1-3; the water is added in an amount of 8-10 times the mass of the sunflower disc powder; the complex enzyme A is added in an amount of 0.8%-1.3% of the mass of the sunflower disc powder; the complex enzyme B is added in an amount of 1.0%-1.5% of the mass of the sunflower disc powder; the chitosan oligosaccharide is added in an amount of 20%-30% of the mass of the sunflower disc small molecule peptide solution; and the transglutaminase is added in an amount of 1%-3% of the mass of the sunflower disc small molecule peptide solution.

[0006] Further, the first enzymolysis is controlled at an enzymolysis temperature of 45-55℃ and a pH of 5.0-6.5, and the enzymolysis is performed for 2-3h; and the second enzymolysis is controlled at an enzymolysis temperature of 35-45℃ and a pH of 7.5-8.0, and the enzymolysis is performed for 1-2h.

[0007] Further, the preparation method of the astragalus extract includes the following steps: astragalus is crushed to obtain astragalus powder, the astragalus powder is soaked in water, and then complex enzyme C is added to perform enzymolysis, ultrasonic extraction, enzyme inactivation, filtration to obtain a filtrate, fermentation of the filtrate by adding lactobacillus, sterilization, filtration, vacuum concentration, addition of propylene glycol alginate, stirring, uniform mixing, drying, crushing, and obtaining of the astragalus extract.

[0008] Further, the mass ratio of the astragalus powder and water is 1:6-8; the added mass of the complex enzyme C is 0.8%-1.2% of the total mass of the astragalus powder and water; the complex enzyme C includes bromelain, β-glucanase, and pectinase with a mass ratio of 6-8:3-5:1-1.5; the added mass of the lactobacillus is 1%-1.5% of the mass of the filtrate; and the added amount of the propylene glycol alginate is 0.1%-0.3% of the mass of the filtrate after filtration.

[0009] Further, the soaking is performed at 40-50℃ for 60-70min; and the ultrasonic extraction is performed at a temperature of 50-60℃ and an ultrasonic power of 400-500W for 30-35min.

[0010] Further, the preparation method of the lettuce extract includes the following steps: the dried lettuce is crushed to obtain lettuce powder, ethanol solution is added to the lettuce powder for heating and reflux extraction, vacuum concentration, drying, and obtaining of the lettuce extract.

[0011] Further, the volume concentration of the ethanol solution is 60%-70%; and the solid-liquid ratio of the lettuce powder and anhydrous ethanol is 1:10-15 mg / mL.

[0012] The preparation method of the composition for reducing uric acid and relieving gout includes the following steps: S1, grind corn silk, tuckahoe, papaya, licorice respectively, and then mix them into a mixture, soak the mixture in water, and then reflux extract 2-3 times, combine the filtrate, reduce pressure concentration, dry, and grind to obtain a mixed extract; S2, mix the mixed extract, sunflower disc small molecule peptide extract, astragalus extract, and lettuce extract, granulate, dry, whole grain, sub-packaging, and packaging to obtain the composition for reducing uric acid and relieving gout.

[0013] Further, in the S1 step, the mass ratio of the mixture to water is 1:8-10; the soaking is performed at 30-40 DEG C for 50-60 min; after the reduction pressure concentration in the S1 step, the relative density of the concentrate is 1.25-1.30; and the drying is performed at 50-60 DEG C.

[0014] Compared with the prior art, the composition has the following beneficial effects: The composition of the present application uses the raw materials of Sanghuang, sunflower disc small molecule peptide extract, corn silk, tuckahoe, papaya, licorice, astragalus extract, and lettuce polypeptide, and prepares the sunflower disc small molecule peptide extract, astragalus extract, and lettuce polypeptide and the composition by a specific preparation method, which can have a synergistic effect, promote the absorption and utilization of active ingredients, effectively regulate the metabolic function to promote the excretion of uric acid, and significantly reduce the content of xanthine oxidase and adenosine deaminase in the liver, thereby effectively reducing the uric acid level, enhancing the immunity of the body, effectively protecting the kidney, avoiding the disorder of kidney function, and achieving good effects of reducing uric acid and relieving gout.

[0015] In the preparation of the sunflower disc small molecule peptide extract, the peptide substances in the sunflower disc can be effectively extracted by multiple enzymatic hydrolysis, and then the stability of the sunflower disc small molecule peptide extract can be improved and the effect of promoting the excretion of uric acid can be promoted under the synergistic action of chitooligosaccharide and transglutaminase. In the preparation of the astragalus extract, the active ingredients in the astragalus can be effectively extracted and promoted to be absorbed and utilized by using the composite enzyme C for enzymatic hydrolysis, and then using the lactobacillus plantarum strain for combined fermentation and extracting under the action of propylene glycol alginate, which can enhance the stability of the astragalus extract, the sunflower disc small molecule peptide extract, and the lettuce extract in maintaining the structure of the intestinal flora, inhibit the generation of harmful bacteria, thereby reducing the generation of uric acid from the intestinal tract, and promoting the excretion of uric acid through the intestinal tract by improving the intestinal barrier function. DETAILED DESCRIPTION

[0016] In order to better understand the technical content of the present application, the following specific examples are provided to further illustrate the present application.

[0017] The experimental methods used in the embodiments of the present application are conventional methods unless otherwise specified.

[0018] The materials, reagents and the like used in the embodiments of the present application can be obtained from commercial channels unless otherwise specified.

[0019] The cellulase, beta-glucanase, alkaline protease, bromelain, pectinase were from Zhejiang Yicun Biotechnology Co., Ltd.; the transglutaminase was from Xi'an Musen Biological Engineering Co., Ltd.; the trypsin was from Shaanxi Linghang Boundary Biological Technology Co., Ltd.

[0020] Embodiment 1 The composition for reducing uric acid and relieving gout of this embodiment comprises the following raw materials by weight: 24 parts of Phellinus baumii, 9 parts of sunflower disc small molecule peptide extract, 7 parts of corn silk, 4 parts of Poria cocos, 2 parts of papaya, 6 parts of licorice, 2 parts of astragalus extract, and 3 parts of lettuce extract.

[0021] The preparation method of the sunflower disc small molecule peptide extract is as follows: the sunflower disc is dried and ground to obtain sunflower disc powder, water is added in an amount of 9 times the mass of the sunflower disc powder, compound enzyme A is added in an amount of 1.3% of the mass of the sunflower disc powder, and compound enzyme B is added in an amount of 1.2% of the mass of the sunflower disc powder; the sunflower disc powder is added with water, compound enzyme A composed of cellulase and beta-glucanase in a mass ratio of 2:2 for primary enzymolysis, then filtered; the primary enzymolysis liquid is taken and added with compound enzyme B composed of trypsin and alkaline protease in a mass ratio of 3:2 for secondary enzymolysis, enzyme is inactivated, filtered again, the secondary enzymolysis liquid is taken and subjected to microfiltration membrane and ultrafiltration membrane to obtain a polypeptide solution with a molecular weight less than 1000 Da, thereby obtaining sunflower disc small molecule peptide solution; chitosan is added in an amount of 20% of the mass of the sunflower disc small molecule peptide solution, and transglutaminase is added in an amount of 3% of the mass of the sunflower disc small molecule peptide solution; the sunflower disc small molecule peptide solution is added with chitosan and transglutaminase and stirred to mix uniformly, enzyme is inactivated, and freeze-drying is performed to obtain the sunflower disc small molecule peptide extract.

[0022] The preparation method of the astragalus extract is as follows: astragalus is crushed to obtain astragalus powder, the astragalus powder is added with water in a mass ratio of 1:7, and compound enzyme C is added in an amount of 1.0% of the total mass of the astragalus powder and water; the astragalus powder is soaked in water at 50℃ for 60 min, and then added with compound enzyme C composed of bromelain, beta-glucanase and pectinase in a mass ratio of 8:3:1.5 for enzymolysis; ultrasonic extraction is performed at a temperature of 60℃ and an ultrasonic power of 500W for 30 min, enzyme is inactivated, then filtered to obtain a filtrate; the filtrate is added with Lactobacillus plantarum in an amount of 1.3% of the mass of the filtrate for fermentation, sterilized, filtered, concentrated under reduced pressure, added with propylene glycol alginate in an amount of 0.3% of the mass of the filtered filtrate, stirred to mix uniformly, and dried to obtain the astragalus extract.

[0023] The preparation method of the lettuce extract is as follows: the dried lettuce is crushed to obtain lettuce powder, 70% ethanol solution is added to the lettuce powder at a solid-liquid ratio of 1:12 (mg / mL), and the mixture is heated to reflux, concentrated under reduced pressure, and dried to obtain the lettuce extract.

[0024] The preparation method of the composition for reducing uric acid and relieving gout comprises the following steps: S1, corn silk, tuckahoe, papaya, and licorice are respectively crushed and mixed into a mixture, water is added to the mixture at a mass ratio of 1:8, and the mixture is soaked at 30℃ for 60 min, followed by reflux extraction for 3 times, the filtrates are combined, concentrated under reduced pressure to a relative density of 1.25, dried at 60℃, and crushed to obtain a mixed extract; S2, the mixed extract, sunflower disc small molecule peptide extract, astragalus extract, and lettuce extract are stirred, granulated, dried, sized, packaged, and packaged to obtain the composition.

[0025] Example 2:

[0026] The composition for reducing uric acid and relieving gout of this example comprises the following raw materials by weight: 18 parts of Phellinus baumii, 10 parts of sunflower disc small molecule peptide extract, 3 parts of corn silk, 6 parts of tuckahoe, 4 parts of papaya, 5 parts of licorice, 4 parts of astragalus extract, and 6 parts of lettuce extract.

[0027] The preparation method of the sunflower disc small molecule peptide extract is as follows: the dried sunflower disc is ground to obtain sunflower disc powder, water is added at a ratio of 10 times the mass of the sunflower disc powder, compound enzyme A is added at a ratio of 0.8% of the mass of the sunflower disc powder, and compound enzyme B is added at a ratio of 1.0% of the mass of the sunflower disc powder, the sunflower disc powder is subjected to one-step enzymolysis with the addition of water and compound enzyme A composed of cellulase and β-glucanase at a mass ratio of 3:1, then filtered, the one-step enzymolysis solution is subjected to two-step enzymolysis with the addition of compound enzyme B composed of trypsin and alkaline protease at a mass ratio of 2:1, the enzyme is inactivated, the two-step enzymolysis solution is filtered again, the polypeptide solution with a molecular weight of less than 1000 Da is obtained by microfiltration and ultrafiltration, then chitosan oligosaccharide is added at a ratio of 30% of the mass of the sunflower disc small molecule peptide solution, and transglutaminase is added at a ratio of 1% of the mass of the sunflower disc small molecule peptide solution, the sunflower disc small molecule peptide solution is stirred and mixed, the enzyme is inactivated, and freeze-drying is performed to obtain the sunflower disc small molecule peptide extract.

[0028] The preparation method of the astragalus extract is as follows: astragalus is crushed to obtain astragalus powder, the astragalus powder and water are mixed at a mass ratio of 1:6, and the complex enzyme C is added in an amount of 0.8% of the total mass of the astragalus powder and water; the astragalus powder and water are soaked at 40°C for 70 min, and then the complex enzyme C composed of bromelain, beta-glucanase and pectinase at a mass ratio of 6:4:1 is added for enzymolysis; the ultrasonic extraction is performed at a temperature of 55°C and an ultrasonic power of 400W for 35 min; the enzyme is inactivated; then the filtrate is obtained by filtration; the filtrate is added with the lactobacillus plantarum at a mass ratio of 1.5% of the mass of the filtrate; the mixture is fermented, sterilized, filtered, concentrated under reduced pressure, and then the propylene glycol alginate is added in an amount of 0.1% of the mass of the filtered filtrate; the mixture is stirred, dried, and then crushed to obtain the astragalus extract.

[0029] The preparation method of the lettuce extract is as follows: the dried lettuce is crushed to obtain lettuce powder; the lettuce powder and anhydrous ethanol are mixed at a solid-liquid ratio of 1:15 mg / mL; the mixture is heated and refluxed with an ethanol solution with a volume concentration of 70%; the mixture is concentrated under reduced pressure, dried, and then crushed to obtain the lettuce extract.

[0030] The preparation method of the composition for reducing uric acid and relieving gout comprises the following steps: S1, corn silk, tuckahoe, papaya and licorice are respectively crushed and mixed into a mixture; the mixture and water are mixed at a mass ratio of 1:10; the mixture is soaked in water at 40°C for 50 min, and then reflux extracted twice; the filtrates are combined, concentrated under reduced pressure to a relative density of 1.30 of the concentrate, dried at 50°C, and then crushed to obtain a mixed extract; S2, the mixed extract, sunflower disc small molecule peptide extract, astragalus extract and lettuce extract are stirred, granulated, dried, sized, packaged and packaged to obtain the composition.

[0031] Example 3:

[0032] The composition for reducing uric acid and relieving gout of the example comprises the following raw materials by weight: 21 parts of phellinus igniarius, 13 parts of sunflower disc small molecule peptide extract, 6 parts of corn silk, 5 parts of tuckahoe, 5 parts of papaya, 3 parts of licorice, 5 parts of astragalus extract and 4 parts of lettuce extract.

[0033] The preparation method of the sunflower disc small molecule peptide extract is as follows: the sunflower disc is dried and ground to obtain sunflower disc powder, water is added in an amount of 8 times the mass of the sunflower disc powder, compound enzyme A is added in an amount of 1.1% of the mass of the sunflower disc powder, and compound enzyme B is added in an amount of 1.5% of the mass of the sunflower disc powder; the sunflower disc powder is added with water, compound enzyme A composed of cellulase and beta-glucanase in a mass ratio of 3:2 for one-step enzymolysis, then filtered, the one-step enzymolysis liquid is taken and added with compound enzyme B composed of trypsin and alkaline protease in a mass ratio of 4:3 for two-step enzymolysis, the enzyme is inactivated, filtered again, the two-step enzymolysis liquid is taken and subjected to microfiltration membrane and ultrafiltration membrane to obtain a polypeptide solution with a molecular weight of less than 1000 Da, thereby obtaining a sunflower disc small molecule peptide solution, then chitosan is added in an amount of 26% of the mass of the sunflower disc small molecule peptide solution, and transglutaminase is added in an amount of 2% of the mass of the sunflower disc small molecule peptide solution, the sunflower disc small molecule peptide solution is added with chitosan and transglutaminase and stirred to mix and react, the enzyme is inactivated, and freeze-drying is performed to obtain the sunflower disc small molecule peptide extract.

[0034] The preparation method of the astragalus extract is as follows: the astragalus is crushed to obtain astragalus powder, the astragalus powder is added with water in a mass ratio of 1:8, and compound enzyme C is added in an amount of 1.0% of the total mass of the astragalus powder and water; the astragalus powder is soaked in water at 50℃ for 60 min, and then added with compound enzyme C composed of bromelain, beta-glucanase and pectinase in a mass ratio of 7:5:1.2 for enzymolysis; ultrasonic extraction is performed at a temperature of 50℃ and an ultrasonic power of 500W for 30 min, the enzyme is inactivated, then filtered to obtain a filtrate; the filtrate is added with lactobacillus plantarum in an amount of 1% of the mass of the filtrate for fermentation, sterilized, filtered, concentrated under reduced pressure, then added with propylene glycol alginate in an amount of 0.2% of the mass of the filtered filtrate, stirred to mix, and dried to obtain the astragalus extract.

[0035] The preparation method of the lettuce extract is as follows: the lettuce is dried and crushed to obtain lettuce powder, the lettuce powder is added with ethanol solution with a volume concentration of 60% in a solid-liquid ratio of 1:13 for heating reflux extraction, concentrated under reduced pressure, dried, and then obtained as the lettuce extract.

[0036] The preparation method of the composition for reducing uric acid and relieving gout comprises the following steps: S1, the corn stigma, the poria cocos, the papaya, the licorice are respectively crushed and mixed into a mixture, the mixture and water are added in a mass ratio of 1:9, the mixture is soaked in water at 40℃ for 60min, and then reflux extraction is carried out for 2-3 times, the filtrate is combined, concentrated under reduced pressure to the relative density of the concentrate is 1.25, dried at 60℃, and then obtained as the mixed extract by crushing; S2, the mixed extract, sunflower disc small molecular peptide extract, astragalus extract, lettuce extract are stirred and mixed, granulated, dried, granulated, packaged, and the composition is obtained.

[0037] Comparative Example 1:

[0038] The difference between this comparative example and Example 3 is that the raw material ratio of the composition is not the same, and the rest is consistent with Example 3.

[0039] The composition for reducing uric acid and relieving gout of this comparative example includes the following raw materials by weight: Phellinus baumii 8 parts, sunflower disc small molecular peptide extract 4 parts, corn silk 12 parts, Poria cocos 13 parts, papaya 10 parts, licorice 3 parts, astragalus extract 9 parts, lettuce extract 3 parts.

[0040] Comparative Example 2:

[0041] The difference between this comparative example and Example 3 is that the raw material does not contain sunflower disc small molecular peptide extract, and the rest is consistent with Example 3.

[0042] The composition for reducing uric acid and relieving gout of this comparative example includes the following raw materials by weight: Phellinus baumii 21 parts, sunflower disc small molecular peptide extract 13 parts, corn silk 6 parts, Poria cocos 5 parts, papaya 5 parts, licorice 3 parts, astragalus extract 5 parts, lettuce extract 4 parts.

[0043] Comparative Example 3:

[0044] The difference between this comparative example and Example 3 is that the raw material does not contain astragalus extract, and the rest is consistent with Example 3.

[0045] The composition for reducing uric acid and relieving gout of this comparative example includes the following raw materials by weight: Phellinus baumii 21 parts, sunflower disc small molecular peptide extract 13 parts, corn silk 6 parts, Poria cocos 5 parts, papaya 5 parts, licorice 3 parts, lettuce extract 4 parts.

[0046] Comparative Example 4:

[0047] The difference between this comparative example and Example 3 is that the sunflower disc small molecular peptide extract does not add chitosan oligosaccharide and transglutaminase stirring and uniform reaction in the preparation process, and the rest is consistent with Example 3.

[0048] The preparation method of the sunflower disc small molecule peptide extract of the comparative example is as follows: the sunflower disc is dried and ground to obtain sunflower disc powder, water is added in an amount of 8 times the mass of the sunflower disc powder, complex enzyme A is added in an amount of 1.1% of the mass of the sunflower disc powder, and complex enzyme B is added in an amount of 1.5% of the mass of the sunflower disc powder, the sunflower disc powder is added with water, complex enzyme A composed of cellulase and β-glucanase in a mass ratio of 3:2 for one-step enzymolysis, and then filtered, the one-step enzymolysis liquid is taken and added with complex enzyme B composed of trypsin and alkaline protease in a mass ratio of 4:3 for two-step enzymolysis, the enzyme is inactivated, filtered again, the two-step enzymolysis liquid is subjected to microfiltration membrane and ultrafiltration membrane to obtain a polypeptide solution with a molecular weight less than 1000 Da, a sunflower disc small molecule peptide solution is obtained, and the sunflower disc small molecule peptide extract is obtained by freeze-drying.

[0049] Comparative Example 5

[0050] The difference between the comparative example and Example 3 is that the enzymolysis process of the sunflower disc small molecule peptide extract is different, and the rest is the same as Example 3.

[0051] The preparation method of the sunflower disc small molecule peptide extract of the comparative example is as follows: the sunflower disc is dried and ground to obtain sunflower disc powder, water is added in an amount of 8 times the mass of the sunflower disc powder, complex enzyme A is added in an amount of 1.1% of the mass of the sunflower disc powder, and complex enzyme B is added in an amount of 1.5% of the mass of the sunflower disc powder, the sunflower disc powder is added with water, complex enzyme A composed of cellulase and β-glucanase in a mass ratio of 3:2, and complex enzyme B composed of trypsin and alkaline protease in a mass ratio of 4:3 for enzymolysis, the enzyme is inactivated, filtered, and the enzymolysis liquid is subjected to microfiltration membrane and ultrafiltration membrane to obtain a polypeptide solution with a molecular weight less than 1000 Da, a sunflower disc small molecule peptide solution is obtained, and then chitosan oligosaccharide is added in an amount of 26% of the mass of the sunflower disc small molecule peptide solution, and transglutaminase is added in an amount of 2% of the mass of the sunflower disc small molecule peptide solution, chitosan oligosaccharide and transglutaminase are added to the sunflower disc small molecule peptide solution and stirred to react, the enzyme is inactivated, and the sunflower disc small molecule peptide extract is obtained by freeze-drying.

[0052] Comparative Example 6

[0053] In the preparation process of the sunflower disc small molecule peptide extract, chitosan oligosaccharide is replaced by glucosamine, and the rest is the same as Example 3.

[0054] The preparation method of the sunflower disc small molecule peptide extract of the comparative example is as follows: the sunflower disc is dried and ground to obtain sunflower disc powder, 8 times the mass of the sunflower disc powder of water is added, 1.1% of the mass of the sunflower disc powder of composite enzyme A is added, 1.5% of the mass of the sunflower disc powder of composite enzyme B is added, the sunflower disc powder is added with water, composite enzyme A composed of cellulase and β-glucanase in a mass ratio of 3:2 for primary enzymolysis, then filtration is performed, the primary enzymolysis solution is taken and added with composite enzyme B composed of trypsin and alkaline protease in a mass ratio of 4:3 for secondary enzymolysis, enzyme inactivation is performed, filtration is performed again, the secondary enzymolysis solution is taken and subjected to microfiltration membrane and ultrafiltration membrane to obtain a polypeptide solution with a molecular weight less than 1000 Da, then sunflower disc small molecule peptide solution is obtained, 26% of the mass of the sunflower disc small molecule peptide solution of amino glucose is added, 2% of the mass of the sunflower disc small molecule peptide solution of transglutaminase is added, the sunflower disc small molecule peptide solution is added with amino glucose and transglutaminase and stirred to mix uniformly, enzyme inactivation is performed, and freeze-drying is performed to obtain the sunflower disc small molecule peptide extract.

[0055] Comparative example 7:

[0056] The difference between the comparative example and example 3 is that the preparation method of the astragalus extract does not contain propylene glycol alginate, and the rest is the same as example 3.

[0057] The preparation method of the astragalus extract is as follows: the astragalus is crushed to obtain astragalus powder, 1:8 of the mass of the astragalus powder and water is added, 1.0% of the total mass of the astragalus powder and water of composite enzyme C is added, the astragalus powder is soaked in water at 50°C for 60 min, then composite enzyme C composed of bromelain, β-glucanase and pectinase in a mass ratio of 7:5:1.2 is added for enzymolysis, ultrasonic extraction is performed at a temperature of 50°C and an ultrasonic power of 500 W for 30 min, enzyme inactivation is performed, then filtration is performed, the filtrate is taken, 1% of the mass of the filtrate of Lactobacillus plantarum is added to the filtrate for fermentation, sterilization is performed, filtration is performed, concentration is performed under reduced pressure, freeze-drying is performed, and crushing is performed to obtain the astragalus extract.

[0058] Comparative example 8:

[0059] The difference between the comparative example and example 3 is that Lactobacillus plantarum is not added for fermentation in the preparation process of the astragalus extract, and the rest is the same as example 3.

[0060] The preparation method of the astragalus extract is as follows: astragalus is crushed to obtain astragalus powder, the astragalus powder and water are mixed at a mass ratio of 1:8, and the complex enzyme C is added in an amount of 1.0% of the total mass of the astragalus powder and water; the astragalus powder and water are soaked at 50°C for 60 min, and then the complex enzyme C composed of bromelain, beta-glucanase and pectinase at a mass ratio of 7:5:1.2 is added for enzymolysis; the ultrasonic extraction is carried out at a temperature of 50°C and an ultrasonic power of 500W for 30 min; the enzyme is inactivated; then the filtrate is obtained by filtration; the filtrate is concentrated under reduced pressure; then 0.2% of propylene glycol alginate based on the mass of the filtrate is added to the filtrate; the propylene glycol alginate is stirred and mixed uniformly in the filtrate; and then the mixture is dried and crushed to obtain the astragalus extract.

[0061] The compositions prepared in Examples 1-3 and Comparative Examples 1-8 are subjected to a mouse uric acid reduction experiment.

[0062] 60 SPF female Kunming mice weighing 20-22 g are randomly divided into a blank group, a model group, Example 1-3 groups and Comparative Example 1-8 groups after adaptive feeding for 3 days, 8 mice in each group.

[0063] During the experiment, the mice in each group are respectively given intragastrically 60 mg·kg -1 of adenine and 200 mg·kg -1 of potassium oxonicate for modeling, once a day, the volume of the drug is 0.2 mL / 10 g, and the continuous administration lasts for 14 days; from the beginning of modeling, the mice in Examples 1-3 and Comparative Examples 1-8 are given intragastrically the compositions prepared in Examples 1-3 and Comparative Examples 1-8, once a day, the dosage of the composition is 2.5 g / kg, the volume of the drug is 20 mL / kg, the blank group and the model group are given intragastrically distilled water with the same volume, and the continuous administration lasts for 14 days; 1 hour after the last administration, the mice are taken out by enucleation, blood samples are taken, and the serum is obtained by centrifugation at 3500 r / min for 10 min; the contents of SUA (serum uric acid), SCr (serum creatinine) and BUN (blood urea nitrogen) in the serum are determined according to the kit instructions; the mice are dissected, the livers are taken out, mixed with physiological saline at a ratio of 1:9, homogenized, and centrifuged at 3500 r / min for 10 min; the contents of XOD (xanthine oxidase) and ADA (adenosine deaminase) in the liver tissue homogenate are determined according to the ELISA kit, and the results are shown in Table 1.

[0064] Table 1

[0065] The results of the above examples 1-3 and comparative examples 1-8 show that the present application extracts sunflower disc small molecule peptide extract and astragalus extract by suitable raw material ratio and using suitable method, so that each raw material plays a synergistic effect, which can reduce the content of serum uric acid, serum creatinine and blood urea nitrogen, and also can significantly reduce the content of xanthine oxidase and adenosine deaminase in liver, effectively reduce the content of uric acid in the body, thereby effectively reducing the uric acid level and relieving the symptoms of gout, and achieving a higher gout relief effect. Compared with example 1, the raw materials of comparative example 2 do not contain sunflower disc small molecule peptide extract, the sunflower disc small molecule peptide extract of comparative example 4 does not add chitosan oligosaccharide and transglutaminase during preparation, the enzymolysis process of the sunflower disc small molecule peptide extract of comparative example 5 is different, and the sunflower disc small molecule peptide extract of comparative example 6 replaces chitosan oligosaccharide with glucosamine during preparation, which all leads to the decrease of the effect of the composition, indicating that the present application can improve the enzymolysis efficiency by using suitable enzymolysis process, and can improve the stability of sunflower disc small molecule peptide extract and the effect of sunflower disc small molecule peptide extract on uric acid excretion under the synergistic effect of chitosan oligosaccharide and transglutaminase, thereby achieving good uric acid lowering effect. Compared with example 1, the raw materials of comparative example 3 do not contain astragalus extract, the preparation method of the astragalus extract of comparative example 7 does not contain propylene glycol alginate, and the preparation process of the astragalus extract of comparative example 8 does not add lactobacillus fermentum for fermentation, which all leads to the decrease of the uric acid lowering effect of the composition, indicating that the present application can improve the stability of astragalus extract in maintaining the structure of intestinal flora, inhibit the generation of harmful bacteria, thereby reducing the generation of uric acid from the intestinal tract, and possibly improve the intestinal barrier function and promote the excretion of uric acid through the intestinal tract, by adding astragalus extract prepared by a specific method and the synergistic effect of propylene glycol alginate during preparation.

[0066] The composition prepared in example 3 of the present application is used for gout subjects to take, the composition of the present application is a powder, the product specification is 6g / bag, 10 bags / box; the taking method is: 1 bag each time, 2 times a day, and taking with warm boiled water.

[0067] Specific diseases are as follows: Subject 1: Mr. Zhang, male, 31 years old, suffering from hyperuricemia, continuously taking 1 box of the composition of the present application, without taking any uric acid lowering drugs during the period, after taking, the uric acid value is reduced from 391μmol / L to 338μmol / L.

[0068] Subject 2: Mr. Shen, 74 years old, has a history of gout for 11 years, after gout attack, the toes cannot walk, and the fingers are limited in movement; after continuously taking the composition of the application for 3 boxes, without taking any uric acid-lowering drugs during the period, after taking, the toes have not been painful, the finger joints have been obviously improved, the movement is not limited, and the uric acid value is reduced from 518 μmol / L to 431 μmol / L.

[0069] Subject 3: Ms. Yang, 71 years old, has high uric acid gout, continuously takes the composition of the application for 1 box, without taking any uric acid-lowering drugs during the period, after taking, the uric acid value is reduced from 484 μmol / L to 449 μmol / L.

[0070] In conclusion, the composition of the application has good uric acid-lowering and gout-relieving effects.

[0071] The above only describes the preferred embodiments of the application and is not intended to limit the application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the application shall be included in the protection scope of the application.

Claims

1. A composition for reducing uric acid and alleviating gout, characterized by comprising, The raw materials include the following components by weight: Phellinus baumii 18-24 parts, sunflower disc small molecule peptide extract 9-13 parts, corn stigma 3-7 parts, Poria cocos 4-6 parts, papaya 2-5 parts, licorice 3-6 parts, astragalus extract 2-5 parts, lettuce extract 3-6 parts; The preparation method of the sunflower disc small molecule peptide extract is as follows: the sunflower disc is dried and ground to obtain sunflower disc powder, water and compound enzyme A are added to the sunflower disc powder for primary enzymolysis, then filtration is performed, the primary enzymolysis solution is taken and compound enzyme B is added for secondary enzymolysis, the enzyme is inactivated, filtration is performed again, the secondary enzymolysis solution is taken and subjected to microfiltration membrane and ultrafiltration membrane to obtain a polypeptide solution with a molecular weight less than 1000 Da, the sunflower disc small molecule peptide solution is obtained, chitosan oligosaccharide and transglutaminase are added to the sunflower disc small molecule peptide solution, stirring is performed, the enzyme is inactivated, and freeze-drying is performed to obtain the sunflower disc small molecule peptide extract.

2. The composition for reducing uric acid and alleviating gout according to claim 1, wherein The compound enzyme A includes cellulase and β-glucanase in a mass ratio of 2-3:1-2; the compound enzyme B includes trypsin and alkaline protease in a mass ratio of 2-4:1-3; the water is added in an amount of 8-10 times the mass of the sunflower disc powder; the compound enzyme A is added in an amount of 0.8%-1.3% of the mass of the sunflower disc powder; the compound enzyme B is added in an amount of 1.0%-1.5% of the mass of the sunflower disc powder; the chitosan oligosaccharide is added in an amount of 20%-30% of the mass of the sunflower disc small molecule peptide solution; and the transglutaminase is added in an amount of 1%-3% of the mass of the sunflower disc small molecule peptide solution.

3. The composition for reducing uric acid and alleviating gout according to claim 1, wherein The primary enzymolysis is controlled at an enzymolysis temperature of 45-55°C, a pH of 5.0-6.5, and an enzymolysis time of 2-3 h; and the secondary enzymolysis is controlled at an enzymolysis temperature of 35-45°C, a pH of 7.5-8.0, and an enzymolysis time of 1-2 h.

4. The composition for reducing uric acid and alleviating gout according to claim 1, wherein The preparation method of the astragalus extract is as follows: astragalus is crushed to obtain astragalus powder, the astragalus powder is soaked in water and then added with compound enzyme C for enzymolysis, ultrasonic extraction is performed, the enzyme is inactivated, then filtration is performed to take the filtrate, the filtrate is added with lactobacillus for fermentation, sterilization is performed, filtration is performed, concentration is performed under reduced pressure, propylene glycol alginate is added, stirring is performed, and then drying, crushing are performed to obtain the astragalus extract.

5. The composition for reducing uric acid and alleviating gout according to claim 4, characterized by, The astragalus powder and water are in a mass ratio of 1:6-8; the compound enzyme C is added in an amount of 0.8%-1.2% of the total mass of the astragalus powder and water; the compound enzyme C includes bromelain, β-glucanase and pectinase in a mass ratio of 6-8:3-5:1-1.5; the lactobacillus is added in an amount of 1%-1.5% of the mass of the filtrate; and the propylene glycol alginate is added in an amount of 0.1%-0.3% of the mass of the filtrate after filtration.

6. The composition for reducing uric acid and alleviating gout according to claim 4, wherein The soaking is performed at 40-50°C for 60-70 min; and the ultrasonic extraction is performed at a temperature of 50-60°C and an ultrasonic power of 400-500 W for 30-35 min.

7. The composition for reducing uric acid and alleviating gout according to claim 1, wherein The preparation method of the lettuce extract is as follows: lettuce is dried and crushed to obtain lettuce powder, ethanol solution is added to the lettuce powder for heating reflux extraction, concentration is performed under reduced pressure, and then drying is performed to obtain the lettuce extract.

8. The composition for reducing uric acid and alleviating gout according to claim 1, wherein The volume concentration of the ethanol solution is 60%-70%; and the solid-liquid ratio of the lettuce powder to anhydrous ethanol is 1:10-15 mg / mL.

9. A method of preparing the composition for reducing uric acid and alleviating gout according to any one of claims 1 to 8, characterized in that, The method comprises the following steps: S1, corn silk, tuckahoe, papaya, licorice are crushed respectively and mixed into a mixture, water is added to the mixture and soaked, then reflux extraction is carried out 2-3 times, the filtrate is combined, concentrated under reduced pressure, dried, crushed, and a mixed extract is obtained; S2, the mixed extract, sunflower disc small molecular peptide extract, astragalus extract, and lettuce extract are stirred, mixed, granulated, dried, whole-grained, packaged, and the composition for reducing uric acid and relieving gout is obtained.

10. The method for preparing the composition for lowering uric acid and relieving gout according to claim 9, characterized in that, In the S1 step, the mass ratio of the mixture to water is 1:8-10; the soaking is carried out at 30-40℃ for 50-60min; in the S1 step, after the concentration under reduced pressure, the relative density of the concentrate is 1.25-1.30; the drying is carried out at 50-60℃.

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