One-bacterium multiple-enzyme bacterial strain as well as screening method and application thereof

Abstract

The invention relates to a one-bacterium multiple-enzyme bacterial strain as well as a screening method and an application thereof. The bacterial strain is bacillus subtilis (Bacillus subtilis 1.1111) and is collected in the China center for type culture collection with the collection number of CCTCC (China center for type culture collection) No: M2011286. The bacillus subtilis (Bacillus subtilis 1.1111) can be used for preparing the bacterial strains of nine enzymes, i.e. xylanase, protease, phytase, pectinase, lipase, sweet dew glucanase, glucoamylase and the like, and the yields of the protease, the sweet dew glucanase, amylase and the glucoamylase are very high. Meanwhile, the bacterial strain is proved to have strong endurance capacity on cholate, artificial gastric juice and artificial intestinal juice by simulating the internal cholate environment, the artificial gastric juice, artificial intestinal juice and the animal test, safety and growth simulation capability are shown to a tested animal, and a foundation is laid for effectively improving the enzyme production capability of the bacterial strain, simultaneously generating the xylanase, the protease, the phytase, the pectinase, the lipase, the sweet dew glucanase and the glucoamylase and realizing one-bacterium multiple-enzyme fermentation in the fermentation process. The mutual synergistic effect among various enzymes generated by the bacterial strain is strong, and the bacterial strain can be used as a feed additive to be applied to agricultural production for livestock, fowls, aquatic livestock and the like.

Description

technical field [0001] The invention relates to a bacterial enzyme strain, and also relates to a screening method and application of the strain, belonging to the technical field of microorganisms. Background technique [0002] Enzyme is a kind of biocatalyst. Feed enzyme preparation is widely used in animal production as a kind of high-efficiency, non-toxic and environmentally friendly "green" feed additive. The application of feed enzyme preparation can not only improve the digestibility and utilization rate of feed , improve the production performance of livestock, poultry and aquatic animals, reduce the content of nitrogen and phosphorus in excrement, and protect water and soil from pollution. It will have a very broad application prospect. [0003] Early enzyme preparations were directly extracted from animals and plants, but due to the long growth cycle of animals and plants and the influence of various factors such as geography, climate and seasons, they were not suita...

AI Technical Summary

Problems solved by technology

The early compound enzyme preparations were used after compounding single enzymes. This method easily caused high production costs, unstable quality, and inconvenient use.

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