Method for extracting curcumin from turmeric

Abstract

The invention discloses a method for extracting curcumin from turmeric, which comprises the following steps: adding a deep eutectic solvent solution into turmeric powder, adding pectinase, uniformly mixing, adding terpilenol, performing magnetic stirring extraction and centrifugation, and taking an upper solution, thereby obtaining the curcumin. According to the method, a solvent system combining a deep eutectic solvent (DES), pectinase and terpilenol is adopted, extraction and enrichment of curcumin in turmeric can be achieved through a simple one-step method, the process is greatly simplified, recycling and reutilization of reagents are achieved, and compared with an existing extraction method, the extraction method has the advantages of being easy to operate, green, environmentally friendly, high in yield and the like. The method is short in extraction time, low in production cost and suitable for large-scale industrial production.

Description

Technical Field

[0001] The invention relates to the technical field of natural active product extraction, and in particular to a method for extracting curcumin from turmeric. Background Art

[0002] Turmeric (Curcuma longa L.) is a plant of the genus Curcuma in the ginger family. It is a dual-purpose food and medicine material. The Compendium of Materia Medica states that turmeric can resist liver damage, lower blood pressure and blood lipids, and relieve pain. It has extremely high medicinal value. Curcumin, a natural dual-purpose food and medicine substance extracted from turmeric, can regulate human physiological functions and has a significant inhibitory effect on tumor cells. Turmeric and curcumin are listed as food additives by the World Health Organization and the Food and Agriculture Organization of the United Nations.

[0003] The extraction methods of curcumin in the prior art include ethanol reflux method, microwave extraction technology, supercritical CO2 extraction method, enzyme extraction technology, etc. These traditional extraction methods have the disadvantages of cumbersome operation steps, low extraction rate, time-consuming and high cost. CN202111317048.1 discloses a method for extracting curcumin by ionic liquid [BMIM] PF6 synergistic enzyme, which uses enzymes to destroy the cell walls of turmeric, promotes the release of curcumin, improves the activity of enzymes by ionic liquids, reduces the reaction activation energy, shortens the enzymatic hydrolysis time, and then uses anhydrous ethanol for vortex extraction, which improves the extraction rate of curcumin. However, this method is also carried out step by step, the operation steps are relatively cumbersome, and it does not involve the recycling of enzymes and ionic liquids, and [BMIM] PF6 is relatively expensive, which is not conducive to large-scale industrial production.

[0004] Therefore, it is necessary to study a method for extracting curcumin which is simple to operate, green and environmentally friendly, has a short extraction time, low production cost, and is suitable for large-scale industrial production. Summary of the invention

[0005] In order to overcome the shortcomings and deficiencies of the prior art, the object of the present invention is to provide a method for extracting curcumin from turmeric, which can achieve the extraction and enrichment of curcumin from turmeric through a simple one-step method, greatly simplifies the process, significantly shortens the extraction time, and can achieve the recovery and reuse of reagents.

[0006] The purpose of the present invention is achieved through the following technical solutions:

[0007] A method for extracting curcumin from turmeric comprises the following steps:

[0008] A low eutectic solvent solution is added to the turmeric powder, and pectinase is added to mix evenly, and then pineneol is added to perform magnetic stirring extraction, centrifuge, and take the upper solution to obtain curcumin.

[0009] The invention adopts a solvent system combining a deep eutectic solvent (DES)-pectinase-pinene alcohol, and utilizes the deep eutectic solvent (DES)-pectinase to synergistically destroy the cell wall of turmeric to release curcumin; and pinene alcohol (LogP2.79) has similar polarity to curcumin (LogP2.92), is immiscible with DES and does not affect the enzyme activity, and can realize the extraction and enrichment of curcumin in turmeric through a simple one-step method, greatly simplifies the process, significantly shortens the extraction time, realizes the reuse of enzymes and DES solvents, is green and environmentally friendly, and has low production cost.

[0010] Preferably, the deep eutectic solvent solution is selected from at least one of a mixture of choline chloride and ethylene glycol in a molar ratio of 1:3-1:5, a mixture of choline chloride and glycerol in a molar ratio of 1:2-1:4, and a mixture of choline chloride and urea in a molar ratio of 1:2-1:5.

[0011] Preferably, the water content of the deep eutectic solvent solution is 40%-60%.

[0012] Preferably, the solid-to-liquid ratio of the turmeric powder to the deep eutectic solvent solution is 1:20-1:50.

[0013] Preferably, the added amount of the pectinase accounts for 1-3% of the mass of the turmeric powder.

[0014] Preferably, the solid-liquid ratio of the turmeric powder to pineneol is 0.1 g: 2-5 ml.

[0015] Preferably, the temperature of the magnetic stirring extraction is 40-60°C, and the extraction time is 30-50 min.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The invention adopts a solvent system combining a deep eutectic solvent (DES)-pectinase-pinene alcohol, and can realize the extraction and enrichment of curcumin in turmeric through a simple one-step method, which greatly simplifies the process and realizes the recycling and reuse of reagents. Compared with the existing extraction method, the extraction method of the invention has the advantages of simple operation, green environmental protection, short extraction time, low production cost, and can be applicable to large-scale industrial production. DETAILED DESCRIPTION

[0018] The present invention is further described below by means of specific implementation methods. The following examples are preferred implementation methods of the present invention, but the implementation methods of the present invention are not limited to the following examples.

[0019] Embodiment 1:

[0020] A method for extracting curcumin from turmeric comprises the following steps:

[0021] Take about 0.1g of turmeric powder, weigh it accurately, add pectinase accounting for 2% of the weight of the turmeric powder, add 2ml of a low eutectic solvent with a water content of 60% (a mixture of choline chloride and ethylene glycol in a molar ratio of 1:4), add 2ml of pineneol, extract with magnetic stirring at 40℃ for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper solution, and obtain curcumin.

[0022] Embodiment 2:

[0023] A method for extracting curcumin from turmeric comprises the following steps:

[0024] Take about 0.1g of turmeric powder, weigh it accurately, add pectinase accounting for 3% of the weight of the turmeric powder, add 3ml of a low eutectic solvent with a water content of 50% (a mixture of choline chloride and glycerol in a molar ratio of 1:3), add 3ml of pineneol, extract at 50℃ with magnetic stirring for 50 minutes, centrifuge at 8000rpm for 10 minutes, take the upper solution, and obtain curcumin.

[0025] Embodiment 3:

[0026] A method for extracting curcumin from turmeric comprises the following steps:

[0027] Take about 0.1g of turmeric powder, weigh it accurately, add pectinase accounting for 1% of the weight of the turmeric powder, add 5ml of a low eutectic solvent with a water content of 70% (a mixture of choline chloride and urea in a molar ratio of 1:2), add 5ml of pineneol, extract with magnetic stirring at 60℃ for 40 minutes, centrifuge at 8000rpm for 10 minutes, take the upper solution, and obtain curcumin.

[0028] Comparative Example 1:

[0029] Take about 0.1 g of turmeric powder, add 2 ml of a low eutectic solvent (a mixture of choline chloride and ethylene glycol in a molar ratio of 1:4) with a water content of 60%, extract with magnetic stirring at 40°C for 30 minutes, centrifuge at 8000 rpm for 10 minutes, take the upper layer solution, and obtain solution ①.

[0030] Comparative Example 2:

[0031] Take about 0.1g of turmeric powder, add 2ml of low eutectic solvent (a mixture of choline chloride and ethylene glycol in a molar ratio of 1:4) with a water content of 60%, add 2ml of pineneol, extract with magnetic stirring at 40℃ for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper solution, and obtain solution ②.

[0032] Comparative Example 3:

[0033] Take about 0.1g of turmeric powder, weigh it accurately, add pectinase accounting for 2% of the weight of the turmeric powder, add 2ml of low eutectic solvent (a mixture of choline chloride and ethylene glycol in a molar ratio of 1:4) with a water content of 60%, add 2ml of ethyl acetate, extract with magnetic stirring at 40℃ for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper solution, and obtain solution ③.

[0034] The upper layer solution prepared in Example 1-3 and Comparative Example 1-3 was fixed to 50 ml, shaken, filtered through a 0.22 um filter membrane into a sample injection vial, and tested by high performance liquid chromatography. The results are shown in Table 1:

[0035] Table 1

[0036] Curcumin(mg / g) Example 1 48.89 Example 2 48.35 Example 3 46.47 Comparative Example 1 8.27 Comparative Example 2 26.27 Comparative Example 3 28.23

[0037] From the results in Table 1, it can be seen that deep eutectic solvent (DES), pectinase and pineneol have a synergistic effect, and the combination of the three can significantly improve the extraction and enrichment of curcumin.

[0038] Embodiment 4: the optimization process of extraction technology is as follows:

[0039] The present invention optimizes the conditions of the main influencing factors to obtain the best extraction conditions. The specific process is as follows:

[0040] 1. Optimization of water content (%) of deep eutectic solvent

[0041] Take about 0.1g of turmeric powder, weigh accurately, add pectinase accounting for 2% of the weight of turmeric powder, add 2ml of low eutectic solvent DES (water content 30-70%), add 2ml of pineneol, stir magnetically at 40℃ for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper layer solution, make up to 50ml, shake well, filter through 0.22um filter membrane into injection vial, and test by high performance liquid chromatography. The results are shown in Table 2:

[0042] Table 2

[0043]

[0044] From the results in Table 2, it can be seen that as the water content of the deep eutectic solvent increases, the content of curcumin increases continuously. When the water content reaches 60%, the content of curcumin reaches the highest or tends to be stable. When the water content reaches 70%, the content of curcumin decreases. Therefore, the water content of the deep eutectic solvent is preferably 40%-60%.

[0045] 2. Optimization of the solid-liquid ratio of turmeric powder and deep eutectic solvent

[0046] Take about 0.1g of turmeric powder, weigh accurately, add pectinase accounting for 2% of the weight of turmeric powder, add low eutectic solvent DES (1-5ml) with a water content of 60%, add 2ml of pineneol, stir magnetically at 40℃ for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper layer solution, make it up to 50ml, shake well, filter it through a 0.22um filter membrane into a sample vial, and test it by high performance liquid chromatography. The results are shown in Table 3:

[0047] Table 3

[0048]

[0049] From the results in Table 3, it can be seen that when the solid-liquid ratio of turmeric powder to the low eutectic solvent is 1:20-1:50, the extraction and enrichment effect is better.

[0050] 3. Optimization of pectinase addition

[0051] Take about 0.1g of turmeric powder, weigh accurately, add pectinase (accounting for 0.5-3% of the weight of turmeric powder), then add 2ml of low eutectic solvent DES with a water content of 60%, add 2ml of pineneol, stir magnetically at 40℃ for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper layer solution, make up to 50ml, shake well, filter through 0.22um filter membrane into a sample vial, and test by high performance liquid chromatography. The results are shown in Table 4:

[0052] Table 4

[0053]

[0054] From the results in Table 4, it can be seen that when the amount of pectinase added is 1-3% of the weight of the turmeric powder, the extraction and enrichment effect of curcumin is better.

[0055] 4. Optimization of magnetic stirring extraction temperature

[0056] Take about 0.1g of turmeric powder, weigh accurately, add pectinase accounting for 2% of the weight of turmeric powder, add 2ml of low eutectic solvent DES with a water content of 60%, add 2ml of pine alcohol, (30-70℃) magnetic stirring extraction for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper layer solution, make up to 50ml, shake well, filter through 0.22um filter membrane into a sample vial, and test by high performance liquid chromatography. The results are shown in Table 5:

[0057] Table 5

[0058]

[0059] From the results in Table 5, it can be seen that as the temperature increases, the content of curcumin increases continuously. When the stirring extraction temperature is 40-60°C, the extraction and enrichment effect of curcumin is better. When the stirring extraction temperature reaches 70°C, the content of curcumin decreases. Therefore, the preferred stirring extraction temperature is 40-60°C.

[0060] 5. Optimization of magnetic stirring extraction time

[0061] Take about 0.1g of turmeric powder, weigh accurately, add pectinase accounting for 2% of the weight of turmeric powder, add 2ml of low eutectic solvent DES with a water content of 60%, add 2ml of pineneol, stir magnetically at 40℃ (10-60 minutes), centrifuge at 8000rpm for 10 minutes, take the upper layer solution, make it up to 50ml, shake well, filter it through a 0.22um filter membrane into a sample vial, and test it by high performance liquid chromatography. The results are shown in Table 6:

[0062] Table 6

[0063]

[0064] From the results in Table 6, it can be seen that when the stirring extraction time is 30-50 min, the extraction and enrichment effect of curcumin is better.

[0065] 6. Optimization of the solid-liquid ratio of turmeric powder and pineneol

[0066] Take about 0.1g of turmeric powder, weigh accurately, add pectinase accounting for 2% of the weight of turmeric powder, add 2ml of low eutectic solvent DES with a water content of 60%, add pineneol (1-5ml), stir magnetically at 40℃ for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper layer solution, make up to 50ml, shake well, filter through 0.22um filter membrane into a sample vial, and test by high performance liquid chromatography. The results are shown in Table 7:

[0067] Table 7

[0068]

[0069] From the results in Table 7, it can be seen that when the solid-liquid ratio of turmeric powder to pinolein is 0.1 g: 2-5 ml, the extraction and enrichment effect of curcumin is better.

[0070] Example 5: Reuse of DES and enzymes

[0071] Take about 0.1g of turmeric powder, weigh accurately, add pectinase accounting for 2% of the weight of turmeric powder, add 2ml of low eutectic solvent DES with a water content of 60%, add 2ml of pine alcohol, stir magnetically at 40℃ for 30 minutes, centrifuge at 8000rpm for 10 minutes, take the upper solution, make up to 50ml, shake well, filter through 0.22um filter membrane into a sample vial; collect the lower solution to make up to 2ml, add 0.1g of turmeric and 2ml of pine alcohol solution, repeat the above experiment, the results are shown in Table 8:

[0072] Table 8

[0073]

[0074] From the results in Table 8, it can be seen that DES and enzyme were recycled three times, and there was no significant difference in the extraction and enrichment of curcumin. After the fourth recycling, there was a significant decrease. Therefore, the present invention not only realizes the extraction and enrichment of curcumin in turmeric by a simple one-step method, greatly simplifies the process, significantly shortens the extraction time, and can simultaneously realize the reuse of enzyme and DES solvent, which is green and environmentally friendly and has low production cost.

Claims

1. A method for extracting curcumin from turmeric, characterized in that: The steps include: A low eutectic solvent solution is added to the turmeric powder, and pectinase is added to mix evenly, and then pineneol is added to perform magnetic stirring extraction, centrifuge, and take the upper solution to obtain curcumin.

2. The method for extracting curcumin from turmeric according to claim 1, wherein: The deep eutectic solvent solution is selected from at least one of a mixture of choline chloride and ethylene glycol in a molar ratio of 1:3-1:5, a mixture of choline chloride and glycerol in a molar ratio of 1:2-1:4, and a mixture of choline chloride and urea in a molar ratio of 1:2-1:

5.

3. The method for extracting curcumin from turmeric according to claim 2, wherein: The water content of the deep eutectic solvent solution is 40%-60%.

4. The method for extracting curcumin from turmeric according to claim 1, wherein: The solid-to-liquid ratio of the turmeric powder to the low eutectic solvent solution is 1:20-1:

50.

5. The method for extracting curcumin from turmeric according to claim 1, wherein: The added amount of the pectinase accounts for 1-3% of the mass of the turmeric powder.

6. The method for extracting curcumin from turmeric according to claim 1, wherein: The solid-liquid ratio of the turmeric powder to pineneol is 0.1 g: 2-5 ml.

7. The method for extracting curcumin from turmeric according to claim 1, wherein: The temperature of the magnetic stirring extraction is 40-60° C., and the extraction time is 30-50 min.

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