New strain of pseudoalteromonas flavipulchra and use thereof

A technology of pseudoalteromonas and bacterial strains, applied in the fields of application, bacteria, bacterial peptides, etc., can solve the problems of breeding animal damage, loss of micro-ecological adjustment effect, etc., achieve obvious antibacterial effect and improve the effect of immune preparations

Inactive Publication Date: 2012-01-04
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, considering the differences in the living environment, growth and development process, and intestinal bacterial flora composition of aquatic animals and terrestrial animals, beneficial bacteria preparations from non-farmed water bodies may cause harm to farmed animals, or fail to become dominant bacteria as soon as possible and lose The effect of microecological regulation

Method used

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  • New strain of pseudoalteromonas flavipulchra and use thereof
  • New strain of pseudoalteromonas flavipulchra and use thereof
  • New strain of pseudoalteromonas flavipulchra and use thereof

Examples

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Effect test

Embodiment 1

[0048] Embodiment 1: Pseudoalteromonas jinlai JG1 bacterial strain is used as feed additive

[0049] 1) Expanded culture of JG1 strain

[0050] First prepare 1L of 2216E liquid medium (5g of peptone, 1g of yeast extract, 0.01g of ferric phosphate, 1000ml of seawater after boiling, adjust the pH value to 7.6-7.8 with 5% sodium hydroxide solution), and then inoculate JG1 strain in the prepared culture medium with shaking at 28°C until OD 600 When it is about 0.5, the culture is stopped and used as JG1 expansion culture medium.

[0051] 2) Feed adult fish with JG1 expanded culture solution as a feed additive

[0052] according to 10 6 Ratio / g of feedstuff JG1 expanded culture solution was mixed into the feedstuff to feed the pufferfish, and the feed intake was increased by more than 50%.

[0053] The JG1 seed solution cultivated overnight was inoculated into the sterilized 2216E liquid medium with 1% inoculum amount, cultured at 150r / min, 28°C for 24h, so that the strain JG1 ...

Embodiment 2

[0055] Embodiment 2: Pseudoalteromonas jinlai JG1 bacterial strain is used as immunopotentiator

[0056] 1) Expanded culture of JG1 strain

[0057] First prepare 1L of 2216E liquid medium (5g of peptone, 1g of yeast extract, 0.01g of ferric phosphate, 1000ml of seawater after boiling, adjust the pH value to 7.6-7.8 with 5% sodium hydroxide solution), and then inoculate JG1 strain in the prepared culture medium with shaking at 37°C until OD 600 When it is about 0.5, the culture is stopped and used as JG1 expansion culture medium.

[0058] 2) Use JG1 expanded culture medium as immune enhancer

[0059] According to the final concentration of 10 5 / ml JG1 expanded culture solution was added to tongue sole seedling water body, the results showed that under the condition of no antibiotics, the survival rate of fry increased by 30% compared with the control group using antibiotics, and the results of salinity test showed that the stress resistance of fry was significant Enhanced,...

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Abstract

The invention relates to a new strain of pseudoalteromonas flavipulchra and use thereof. The new strain is the JG1 strain of the pseudoalteromonas flavipulchra, and the collection number of the JG1 strain of pseudoalteromonas flavipulchra is CGMCC No.5009; and the JG1 strain produces Pfa protein during metabolism. The JG1 strain of pseudoalteromonas flavipulchr, which is disclosed by the invention, can achieve antibacterial effects by means of bacteriocin protein, small molecular compounds and the like and can inhibit various common pathogenic bacteria in aquaculture animals; and the results of animal tests on zebra fish, mantis shrimps, scallops, clams and the like indicate that JG1 has neither toxic nor side effects on animals and that the bacterial preparation prepared by using the JG1 can be effectively used in actual production as a synergist in aquiculture. The Pfa protein produced in the metabolism of the JG1 strain has an obvious antibacterial effect and can be used as a preparation for improving the immunity in aquaculture animals.

Description

technical field [0001] The invention belongs to the technical field of screening beneficial microorganisms in aquatic products, and in particular relates to a new strain of Pseudoalteromonas aureus and application thereof. Background technique [0002] As a fast-growing industry in the contemporary era, aquaculture not only brings abundant aquatic food, but also creates trillions of dollars in economic value. According to the data report of the Food and Agriculture Organization of the United Nations (FAO), the total output of the world's aquaculture industry in 2006 reached 51.63 million tons, accounting for 36% of the total world fishery output in 2006. Since 1970, the scale of the world's aquaculture industry has grown at an average annual rate of 9.2%, while in contrast, the average annual growth rate of the capture industry and land-based aquaculture industry is only 1.4% and 2.8%, respectively. [0003] However, while developing rapidly and creating huge wealth, the aq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31C07K14/195A23K1/16C12R1/01A23K20/147A23K50/80
Inventor 张晓华史晓翀于敏汤开浩金光
Owner OCEAN UNIV OF CHINA
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