The present invention provides a method for preparing paraffin sections of
mantis shrimp eyeballs, comprising the following steps: step one: sampling; step two: fixation selection: fix with 4-6%
paraformaldehyde at
room temperature for 25-35 minutes; step three:
dehydration treatment; step four: embedding the
wax-impregnated tissue block with a modified
paraffin wax; step five: continuously sectioning the sample using a
microtome; step six: flattening the slices in a 37°C water bath; step seven: dewaxing and rehydrating after incubation at 37°C for 6-12 hours; step eight: after
staining the sample, sealing the sample with a 50% sterile
glycerol aqueous solution. The method for preparing paraffin sections of
mantis shrimp eyeballs of the present invention comprises the improvement and optimization of procedures such as the
dissection of the
mantis shrimp eyeball shell, the selection of a fixative,
dehydration, and
waxing, breaking through the technical bottlenecks such as incomplete
dehydration and
waxing caused by the difficulty of dissecting the
mantis shrimp eyeball due to the shell being difficult to dissect, damage to the blade due to the hard shell, and fragmentation of the slices.