Non-invasive method for isolation and detection of fetal DNA

a fetal dna and non-invasive technology, applied in the field of non-invasive methods for isolation and detection of fetal dna, can solve the problems of not being able to distinguish fetal cells from maternal cells, not being able to confirm the presence of fetal dna in cells, and not being able to successfully isolate cells subsequently shown to contain fetal dna
US20060051775A1Inactive Publication Date: 2006-03-09CHILDRENS HOSPITAL
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
CHILDRENS HOSPITAL
Publication Date
2006-03-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

A method of detecting the presence or absence of the fetal DNA sequence of interest in fetal DNA derived from a sample of peripheral blood obtained from a pregnant woman is described. The method involves obtaining a sample peripheral blood from a pregnant woman, treating the sample of peripheral blood such that the fetal DNA present in the fetal nucleated cells is made available for detection and detecting the presence or absence of the fetal DNA sequence of interest in the available fetal DNA. The proportion of fetal nucleated cells present in the sample of peripheral blood can be increased forming a sample enriched in fetal nucleated cells prior to the detection step. The fetal DNA sequence of interest can be detected by treating the peripheral blood sample such that fetal DNA present in the sample is made available for hybridization with a DNA probe and subsequently contacting the available fetal DNA with a DNA probe hybridizable to fetal DNA of interest under hybridization conditions. The presence or absence of hybridization between the DNA probe and the fetal DNA of interest is detected as an indication of the presence or absence of the fetal DNA of interest.
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Description

RELATED APPLICATIONS

[0001] This application is a continuation-in-part application of U.S. Ser. No. 07 / 772,689, filed on Oct. 7, 1991, which is a continuation-in-part application of U.S. Ser. No. 07 / 706,393, filed on May 28, 1991, now abandoned, which is a continuation-in-part of U.S. Ser. No. 07 / 436,057, filed on Nov. 13, 1989, now abandoned, all being entitled “Non-Invasive Method for Isolation and Detection of Fetal DNA” by Diana W. Bianchi. The contents of all of the forementioned applications are hereby expressly incorporated by reference.FUNDING

[0002] Work described herein was supported by the National Institute of Health and Children's Hospital Medical Center. BACKGROUND

[0003] A variety of fetal cell types—platelets, trophoblasts, erythrocytes and leucocytes—cross the placenta and circulate transiently within maternal blood (Schroder, J., J. Med. Genet. 12:230-242 (1975); Douglas G. W. et al., Am. J. Obstet. Gynec., 78:960-973 (1959)). There have been numerous reports of ef...

Claims

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