Preparing method for magnetic control ratio fluorescence adapter sensor for sensitivity detection of fumonisins B1
An aptamer sensor, sensitive detection technology, applied in fluorescence/phosphorescence, instrumentation, biological testing, etc., can solve the problems of high cost, low sensitivity, and easily disturbed probe concentration.
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[0039] (1) Preparation of gQDs aqueous solution and rQDs aqueous solution:
[0040] Prepare gQDs and rQDs according to the existing method, specifically as follows: Weigh 0.0638g tellurium powder and 0.0449g sodium borohydride into a 10mL colorimetric tube, add 4mL ultrapure water, pass N 2 After deoxygenation for 15 min, place in a refrigerator at 4° C. to react to obtain a sodium hydride telluride (NaHTe) solution. 0.1142gCdCl 2 2.5H 2 Add O and 75 μL of mercaptopropionic acid to a three-neck flask containing 50 mL of secondary water, stir magnetically for 5 min under nitrogen protection, adjust the pH of the solution to 8.5 with 2M NaOH, quickly add 2 mL of the NaHTe solution prepared above, continue stirring for 30 min, and reflux at 100 °C 0.5h and 12h, gQDs were obtained after reflux for 0.5h, and rQDs were obtained after reflux for 12h. Stop the reaction and return to room temperature, add 100mL of ethanol to stir and then let stand for 15min. After centrifugation, ...
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