Preparing method for magnetic control ratio fluorescence adapter sensor for sensitivity detection of fumonisins B1

An aptamer sensor, sensitive detection technology, applied in fluorescence/phosphorescence, instrumentation, biological testing, etc., can solve the problems of high cost, low sensitivity, and easily disturbed probe concentration.

Inactive Publication Date: 2016-06-29
JIANGSU UNIV
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Problems solved by technology

[0005] The present invention aims at inventing a magneto-controlled ratiometric fluorescent aptasensor with the advantages of simple process and low cost, and provides a method for preparing high-selectivity, high-precision, and wide-ranging measurement range for highly sensitive detection of FB1, which solves the problem of current There are problems such as high cost of detection methods, complex detection schemes, long detection time, low sensitivity, changes in excitation intensity and susceptible to interference of probe concentration

Method used

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  • Preparing method for magnetic control ratio fluorescence adapter sensor for sensitivity detection of fumonisins B1
  • Preparing method for magnetic control ratio fluorescence adapter sensor for sensitivity detection of fumonisins B1

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Embodiment 1

[0039] (1) Preparation of gQDs aqueous solution and rQDs aqueous solution:

[0040] Prepare gQDs and rQDs according to the existing method, specifically as follows: Weigh 0.0638g tellurium powder and 0.0449g sodium borohydride into a 10mL colorimetric tube, add 4mL ultrapure water, pass N 2 After deoxygenation for 15 min, place in a refrigerator at 4° C. to react to obtain a sodium hydride telluride (NaHTe) solution. 0.1142gCdCl 2 2.5H 2 Add O and 75 μL of mercaptopropionic acid to a three-neck flask containing 50 mL of secondary water, stir magnetically for 5 min under nitrogen protection, adjust the pH of the solution to 8.5 with 2M NaOH, quickly add 2 mL of the NaHTe solution prepared above, continue stirring for 30 min, and reflux at 100 °C 0.5h and 12h, gQDs were obtained after reflux for 0.5h, and rQDs were obtained after reflux for 12h. Stop the reaction and return to room temperature, add 100mL of ethanol to stir and then let stand for 15min. After centrifugation, ...

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Abstract

The invention provides a preparing method for a magnetic control ratio fluorescence adapter sensor for sensitivity detection of fumonisins B1. The method comprises the following steps that a CdTe quantum dot aqueous solution emitting green fluorescence and a CdTe quantum dot aqueous solution emitting red fluorescence are prepared; a SiO2 nanosphere dispersing solution is prepared; a Fe3O4@SiO2 magnetic bead dispersing solution is prepared; a gQDs-covered SiO2 dispersing solution is prepared; an rQDs-covered mSiO2 nanosphere dispersing solution is prepared; a SiO2@gQDs-marked Aptamer fluorescence probe dispersing solution is prepared; an mSiO2@rQDs-marked cDBA magnetic-fluorescence probe dispersing solution is prepared; the sensor is structured, and a sample is detected; the sample to be detected is detected. The magnetic control ratio fluorescence adapter sensor developed through the preparing method is simple in processing method, convenient to use, low in detection cost, and capable of providing internal correction to eliminate interferences of factors such as environment factors, stimulating strength changes and probe concentration, greatly reducing errors, and improving accuracy and sensitivity of FB1 detection.

Description

technical field [0001] The invention relates to the fields of material science, optical analytical chemistry, nano-biological sensing and the like, in particular to a preparation method of a magnetically controlled ratiometric fluorescent aptasensor for sensitive detection of fumonisin B1. Background technique [0002] Fumonisin (Fumonisin) is a secondary metabolite produced by Fusarium moniliforme, Fusarium laminarum, and Verticillium, and is a class of diester compounds with similar structures composed of different polyhydric alcohols and glyceric acid. So far, humans have discovered 28 fumonisin analogues, which are divided into four categories: A, B, C, and P. Among them, Fumonisin B1 (Fumonisin B1, FB1) is widely distributed in nature and is more toxic. FB1 pollution is mainly corn and its processed products, in addition to some foods such as rice, millet, sorghum, milk, beer and so on. FB1 can induce the occurrence of esophageal cancer, liver cancer, and gastric cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/64
CPCG01N21/6428G01N33/5306G01N33/5308G01N2021/6439
Inventor 钱静华梦娟王坤王成全刘倩郝楠
Owner JIANGSU UNIV
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