Microfluidic chip for enriching microorganisms in air and preparation method thereof

A microfluidic chip and microbial technology, applied in biochemical equipment and methods, biological material sampling methods, microbial measurement/inspection, etc., can solve the problems of inability to separate non-biological particles from microorganisms, large volume, incompatibility with chips, etc. problems, to achieve rapid enrichment, small volume effect

Inactive Publication Date: 2010-06-30
FUDAN UNIV
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Problems solved by technology

However, the existing gas collection devices have disadvantages such as large volume, inability to effectively separate non-biological particles from microorganisms, complicated subsequent sample processing, and incompatibility with micro-rapid detection devices such as chips.

Method used

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  • Microfluidic chip for enriching microorganisms in air and preparation method thereof
  • Microfluidic chip for enriching microorganisms in air and preparation method thereof

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Embodiment Construction

[0031]1. Substrate preparation. Put the silicon wafer into Piranha solution (98% concentrated sulfuric acid: 30% hydrogen peroxide=7:3, volume ratio) and boil for 15 minutes. Rinse five times with deionized water, blow dry with nitrogen, and bake at 200 °C for 30 min.

[0032] 2. Shake the paint. Pour Microchem's SU-8 glue (the same below) on the center of the silicon wafer, and tilt the silicon wafer so that SU-8 covers most of the silicon wafer. Let it stand for 15 minutes while eliminating the air bubbles generated during the pouring process. Use a spin coater (Spin-Coater KW-4A, Chemat Technology, Inc.) to perform two progressive spin coatings: 500 rpm spin coating for 15 seconds, 3000 rpm spin coating for 30 seconds, and standing for 10 minutes to alleviate the edge protrusion effect .

[0033] 3. Soft baking. Gradually increase to 95°C at a rate of 5°C / min on a hot plate, during which time it is kept at 65°C and 95°C for 3 min and 6 min, respectively. Then slowly l...

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Abstract

The invention belongs to the technical field of bioanalysis and detection, in particular to a microfluidic chip for enriching microorganisms in air and a preparation method thereof. The chip uses optically transparent polydimethylsiloxane, and the like as materials by adopting a molding method, and the chip internally contains a sample enriching channel and a gas channel to realize the collectionof biologic grains of bacteria, fungi, viruses, and the like in an air sample. An enriched sample can be combined with a PCR (polymerase chain reaction) microfluidic chip and an immune microfluidic chip to carry out gene analysis or immunoassays so as to achieve the quick detection of the contents and the species of the microorganisms in the air. The invention has the characteristics of high speed, high efficiency, convenient taking, low price and easy automation control, and the integration of the microfluidic chip with other chips can complete automatic signal acquisition, remote transmission and signal analysis, thus the microfluidic chip can be used for the field quick detection and remote control detection within a large range on microorganisms in the air or other gases.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and detection, in particular to a microfluidic chip used for on-site rapid enrichment of microorganisms in the air, and provides a preparation method of the chip. Background technique [0002] In recent years, human beings are faced with the grim reality of the global epidemic of infectious diseases: the emergence of new infectious diseases, the re-ravage of old infectious diseases, biological attacks, and the occurrence and prevalence of human-induced infectious diseases. Among the emerging infectious diseases, air aerosol-mediated respiratory infectious diseases account for the main position, which is a key and difficult issue in the prevention and control of infectious diseases. The pathogens that cause respiratory infections are transmitted through ambient air aerosols and lead to human infections. Compared with infectious disease pathogens transmitted by other means, they are more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12Q1/70C12Q1/68C12Q1/04G01N33/53
Inventor 隋国栋赵望刘思秀张金玲刘超
Owner FUDAN UNIV
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