Method for preparing galvanic deposit calcium phosphorus mineralized layer superfine fibre bone material

An ultra-fine fiber, electrodeposition technology, applied in medical science, prosthesis, etc., can solve the problems of unsuitable bioactive component loading, composite fiber degradation, long time, etc., achieve short preparation time, mild conditions, good phase capacitive effect

Inactive Publication Date: 2009-04-15
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mineralization process takes a long time, which is easy to cause the degradation of composite fibers, and is not suitable for the loading of bioactive components.

Method used

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  • Method for preparing galvanic deposit calcium phosphorus mineralized layer superfine fibre bone material
  • Method for preparing galvanic deposit calcium phosphorus mineralized layer superfine fibre bone material
  • Method for preparing galvanic deposit calcium phosphorus mineralized layer superfine fibre bone material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Using chloroform / acetone with a volume ratio of 3:1 as solvent, prepare 6% (w / v) PLLA (molecular weight: 130,000) electrospinning solution;

[0034] (2) A 2cm×2cm platinum electrode was selected, and the surface was polished and cleaned as an electrospinning collection device, and PLLA ultrafine fibers were prepared on the surface of the electrode by single-nozzle electrospinning technology. A 21G injection needle (inner diameter 0.51mm) is used as the spinneret, a high-voltage electrostatic generator provides DC high voltage, and a micro-injection pump delivers the electrospinning solution; the electrospinning parameters are: the voltage is 16.0KV, the receiving distance is 15.0cm, and the liquid supply rate is 1.2ml / h, after 30 minutes, the surface of the electrode is covered with a PLLA fiber membrane with a thickness of about 0.1mm, and the other side of the electrode is replaced with a PLLA fiber membrane of the same thickness;

[0035] (3) Prepare 100ml electr...

Embodiment 2

[0039] (1) The material used is type I collagen (molecular weight 0.8-1.0×10) purchased from Sichuan Mingrang Biotechnology Co., Ltd. 5 ), using hexafluoroisopropanol as a solvent to prepare an electrospinning solution with a concentration of 8% (w / v);

[0040] (2) Receive electrospun collagen fibers on both sides of platinum electrodes. The electrospinning parameters are: the voltage is 15KV, the receiving distance is 10cm, the liquid supply rate is 0.8ml / h, and the receiving time of the single-sided electrode is 30min. After the collagen fibers are collected on both sides of the electrode, place it in a vacuum drying oven at room temperature for 12 hours to remove residual solvent;

[0041] The rest is the same as in Example 1, and the mineralized collagen porous bone material is prepared.

Embodiment 3

[0043] The same as in Example 1, the difference is that the coaxial electrospinning technology is used to prepare skin-core electrospun ultrafine fibers on both sides of the platinum electrode. The coaxial co-spinning device in this embodiment uses a 21G injection needle as the inner needle (inner diameter 0.51 mm, outer diameter 0.8 mm), and the inner diameter of the outer spray needle is 1.6 mm. The inner injection needle is indented about 5mm inward. The cortex material was made of 10% (w / v) PLLA electrospinning solution, the core material was made of 3% PEO added with recombinant human bone morphogenetic protein-2 (rhBMP-2) (80-100 μg / mL electrospinning solution), and chloroform and sterile saline as solvents to prepare skin and core electrospinning solution. During coaxial co-spinning, the output voltage is controlled to be 18KV, the supply speeds of the skin and core layer solutions are 3ml / h and 0.4ml / h respectively, and the receiving distance is 12cm.

[0044] The re...

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Abstract

The invention relates to a method for preparing superfine fiber bone materials of an electrodeposition calcium phosphate mineralized layer, which comprises the following steps: firstly, high-polymer superfine fibers are prepared on the surface of a metal electrode by adoption of electrostatic spinning and taken as an electrochemical deposition template; a layer of bone salt ingredients which are rich in calcium and phosphor is deposited on the surface of the fibers by the constant-voltage or constant-current deposition technology; mineralized superfine fibers are soaked into 0.1 to 1.0 mol per liter of NaOH solution for 1 to 24 hours; mineralized electro spinning fibers are subjected to die stamping into blocks under the pressure of between 10 and 40 MPa; and the porous bone materials with a porosity between 50 and 90 percent and a pore diameter between 50 and 500 micrometers are processed by the salt particle leaching / gas foaming technology, and are used for bone defect restoration after freeze-drying sterilization. The porous bone materials prepared by the method have better biocompatibility; and the electrochemical deposition technology can prepare organic-inorganic composite materials with higher bone salt content within a shorter period; and the preparation time is short and the preparation conditions are mild.

Description

technical field [0001] The invention belongs to the field of preparation of electrospun superfine fiber bone material, in particular to a preparation method of superfine fiber bone material with electrodeposited calcium phosphate mineralization layer. Background technique [0002] Bone defects caused by various reasons, especially the treatment of large-scale bone defects are still a major problem in clinical medicine. Traditional autologous or allogeneic bone transplantation has disadvantages such as limited bone supply, low osteogenesis ability, easy to cause immune reaction and disease transmission, and cannot meet the requirements of clinical treatment. [0003] The main component of natural bone is a biomineralization system composed of collagen fibers and hydroxyapatite (HA) crystals. In terms of structure, natural bone is an organic-inorganic composite material with a complex hierarchical structure formed by collagen fibers running through HA. Collagen fibers with a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/56C04B35/80
Inventor 何创龙邱立军徐晓红王红声莫秀梅
Owner DONGHUA UNIV
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