Preparation and application of 1, 8-naphthalimide hydrogen sulfide fluorescent molecular probe

Abstract

The invention discloses a preparation method of a 1, 8-naphthalimide hydrogen sulfide fluorescent molecular probe and application of the 1, 8-naphthalimide hydrogen sulfide fluorescent molecular probe in hydrogen sulfide recognition and detection. The fluorescent molecular probe with double recognition sites is obtained by using N-(p-benzyl)-4-hydroxy-1, 8-naphthalimide as a fluorescent matrix and 2, 4-dinitrophenyl ether as a recognition group, hydrogen sulfide performs thiolysis on the 2, 4-dinitrophenyl ether, so that hydroxyl is exposed out of a fluorophore, and the fluorescence intensity is enhanced (by more than 30 times). The fluorescent molecular probe has the advantages of stable photochemical properties, high fluorescence quantum yield, low price and the like, specifically recognizes hydrogen sulfide, is not interfered by ions such as Na<+>, Ca<2+>, NO2<->, H2O2, ClO<->, HSO4<->, CN2H4S, HSO3<->, S2O5<2-> and S2O8<2->, has a linear detection range increased to 0-40 [mu] mmol / L, can be used for quantitative detection of high-concentration hydrogen sulfide in river water, rainwater, drinking water, drilling fluid, marsh liquid and the like, and has potential practical application value.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to a preparation method of a novel 1,8-naphthalimide hydrogen sulfide fluorescent molecular probe and its application in hydrogen sulfide identification and detection. Background technique [0002] As one of the three major gas molecules, hydrogen sulfide (H 2 S) are closely related to human health, plant growth and environmental issues. In terms of human health, intracellular H 2 S plays an important role in the regulation of digestive system, nervous system, cardiovascular system and urinary system, H 2 S concentration imbalance can lead to arterial and pulmonary hypertension, Alzheimer's disease, bone damage, liver cirrhosis and other diseases. Exogenous H 2 S is also considered to be a highly toxic drug next to cyanide, and low concentrations of H 2 S can regulate the content of osmotic regulator factors in plants and maintain the plant's...

AI Technical Summary

Problems solved by technology

But most of them have only one recognition site, and the fluorescence intensity is related to H 2 The linear range of S concentration is narrow (between 0-20 μmmol / L), which limits its use at high concentrations of H 2 Applications in the S environment, so design new fluorescent molecular probes, broaden the H 2 The linear detection range of S is of great significance

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