Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescence probe for quickly identifying thiophenol

A fluorescent probe and thiophenol technology, which is applied in the field of chemical analysis, can solve the problems of poor water solubility of fluorescent probes, insufficient response speed, and weak anti-interference ability, and achieves strong anti-interference ability, rapid response, and low response speed. quick effect

Active Publication Date: 2019-01-08
WUHAN INSTITUTE OF TECHNOLOGY
View PDF8 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, although the current thiophenol fluorescent probes have realized the detection of thiophenol in living cells, the response speed to thiophenol in the detection process is not fast enough, and at the same time, most of the fluorescent probes are relatively water-soluble. Poor and anti-interference ability is not strong, which leads to the limitation of its scope of use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence probe for quickly identifying thiophenol
  • Fluorescence probe for quickly identifying thiophenol
  • Fluorescence probe for quickly identifying thiophenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for preparing a fluorescent probe for rapid detection of thiophenol, specifically comprising the following steps:

[0038] Synthesis of Intermediate A: Dissolve 1 g (6.84 mmol) of 2,7-naphthyridin-1(2H)-one and 9.81 g (34.21 mmol) of phosphorus oxybromide in 10 mL of acetonitrile and heat to 80 ℃ for 1 h. Add ice water to the reaction system to quench the phosphorus oxybromide, then adjust the pH to neutral. The solution was extracted 3 times with dichloromethane, then with Na 2 SO 4 The dichloromethane extract was dried and distilled under reduced pressure to obtain a red crude product, which was further purified by silica gel column chromatography to obtain a white solid, namely Intermediate A (980 mg, yield 69%). 1H NMR (400 MHz, CDC13): δ 9.76 (s, 1H), 8.82 (d, J = 4 Hz, 1H),8.48 (d, J = 4 Hz, 1H), 7.68 (d, J = 8 Hz, 1H), 7.61 (d, J = 8 Hz, 1H).

[0039] Synthesis of Intermediate B: Under argon, 400 mg (1.91 mmol) of Intermediate A and 134 mg (0.19 mmo...

Embodiment 2

[0046] The effect of the fluorescent probe obtained in Example 1 reacting with thiophenol was determined.

[0047] A 100 μM fluorescent probe solution in Example 1 was prepared using 10 mM PBS (pH=7.4) buffer solution, and a 10 mM thiophenol stock solution was prepared with acetonitrile. Take 100 μL of the above-mentioned fluorescent probe solution and 900 μL PBS (pH=7.4) buffer solution in a cuvette, and detect its fluorescence spectrum under 355 nm excitation, as shown in Figure 4 The solid line part; take 100 μL of the above compound f solution, 895 μL of PBS (pH=7.4) buffer solution and 5 μL of thiophenol stock solution in a cuvette, and detect its fluorescence spectrum under 355 nm excitation, as shown in Figure 4 imaginary part.

[0048] from Figure 4 Comparing the dotted line and the solid line, it can be seen that the background of the fluorescent probe is low and the response to thiophenol is strong, which is a fluorescence-enhanced thiophenol probe.

Embodiment 3

[0050] The analysis of the reaction of the fluorescent probe obtained in Example 1 with different concentrations of thiophenol was determined.

[0051] Add 100 μL of fluorescent probe solution and PBS (pH=7.4) buffer solution to the cuvette, and then add working concentrations of 0, 2, 4, 6, 8, 10, 20, 40, 60, 80, 90 , 100 μM thiophenol was prepared into a 1 mL reaction system, and the fluorescence spectra of different concentrations of thiophenol reacted with the fluorescent probe for 30 seconds were measured, as shown in Figure 5 shown.

[0052] from Figure 5 It can be seen that within a certain concentration range, the fluorescence intensity of the reaction system at 580 nm increases with the concentration of thiophenol, indicating that the probe can detect thiophenol at different concentrations.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fluorescence probe for quickly identifying thiophenol. The fluorescence probe reacts with the thiophenol to generate a reaction product with high fluorescence. The fluorescence probe can quickly react with the thiophenol thorugh a 2,4-dinitrophenyl ether base identification group with relatively high electron absorbing capacity and generate the reaction product with highfluorescence, and is high in sensitivity and high in response speed; the fluorescence probe is relatively high in interference resistance, can specifically identify the thiophenol and is applicable tothiophenol detection of various samples with relatively high interference strength.

Description

technical field [0001] The invention relates to the technical field of chemical analysis, in particular to a fluorescent probe for rapidly identifying thiophenol. Background technique [0002] Thiophenol (PhSH) is a highly toxic pollutant that widely exists in the preparation of pharmaceuticals, pesticides and chemical intermediates. Thiophenol can cause a series of serious harm to other organisms such as humans, mice, and fish through inhalation, ingestion, and percutaneous absorption. Thiophenol has a strong irritating effect on eyes and skin; it can cause breathing difficulties, coma and even death; high concentration of thiophenol can cause the death of fish and mice. Therefore, establishing a specific detection method for thiophenol in the environment and in organisms has important practical application value. [0003] At present, the detection methods of thiophenol include high performance liquid chromatography, gas chromatography, electrochemical method, ultraviolet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D471/04C09K11/06G01N21/64
CPCC07D471/04C09K11/06C09K2211/1029G01N21/643
Inventor 孙琦佘能芳任瑞
Owner WUHAN INSTITUTE OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com