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Sialic acid bonded with antigens and application of sialic acid

A sialic acid and antigen technology, applied in the field of sialic acid, can solve the problem that N-acetyl mannosamine has no cell and tissue specificity

Active Publication Date: 2015-11-04
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its disadvantage is that N-acetyl mannosamine has no cell and tissue specificity, and it can be expressed on the cell surface of various tissues of animals 21-23

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  • Sialic acid bonded with antigens and application of sialic acid
  • Sialic acid bonded with antigens and application of sialic acid
  • Sialic acid bonded with antigens and application of sialic acid

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specific Embodiment

[0057] 1. Laser confocal fluorescence microscopy analysis of DNP-Sia covalently expressed on the surface of cancer cells

[0058]The B16F10 cells were subcultured into 35mm glass-bottomed (NEST) special confocal culture dishes, and cultured in DMEM containing 10% FBS for 24 hours. DNP-Sia (100 μmol / L) was added or not added to these cells, and incubated for 0-24 hours. Incubate with biotinylated Anti-DNP IgG (10 μg / mL) for 15 minutes at 4°C, then stain with phycoerythrin-streptavidin (10 μg / mL) for 15 minutes, and observe DNP-Sia in the cells with a confocal laser fluorescence microscope of the distribution.

[0059] 2. Flow cytometric analysis of DNP-Sia content on the surface of cancer cells

[0060] The B16F10 cells were subcultured into 12-well plates (NEST), and cultured for 24 hours by adding DMEM containing 10% FBS. DNP-Sia (100 μmol / L) was added or not added to these cells, incubated for 0-24 hours, and washed twice with PBS. Incubate with biotinylated Anti-DNP IgG...

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Abstract

The invention discloses a sialic acid bonded with antigens and application of the sialic acid and relates to sialic acids. The sialic acid bonded with the antigens is composed of 2,4-dinitrobenzene and a sialic acid body. The molecular formula is C17H22N4O12. The molecular weight is 474.1234. The sialic acid bonded with the antigens can be applied to preparation of drugs for immunity prevention and treatment of tumors. After being absorbed by tumor cells, the sialic acid bonded with the antigens is expressed on the surfaces of the tumor cells in a covalency mode through a metabolic pathway. DNP-Sia is intravenously injected to a mouse body having subcutaneous tumors and is fast and effectively expressed on the surfaces of the tumor cells of solid tumors. The tumor cells where the DNP-Sia is expressed can effectively collect DNP antibodies or T cells with cytotoxicity and are combined with the DNP antibodies or the T cells with cytotoxicity, and therefore the tumor cells can be dead. Relevant antigens on the surfaces of the tumors are expanded, and growth of the tumors can be effectively regulated and controlled.

Description

technical field [0001] The present invention relates to sialic acid, in particular to an antigen-linked sialic acid and its application. Background technique [0002] The body's immune system destroys "non-self" cells by identifying specific markers on the cell surface, thereby protecting "self" cells. However, "non-me" cancer cells use a variety of ways to evade recognition by the immune system. In order to improve the efficiency of the immune system to kill tumors, a lot of work is in the modification of lymphocytes 1-4 . For example, T cells express chimeric antigen receptors (CAR) through genetic engineering to obtain cytotoxic T cells (CAR T). Since CAR is a fusion protein of the variable region of a single-chain antibody and a T cell signaling molecule, it enables T cells to recognize specific antigens on the surface of tumor cells in a non-MHC-restricted manner and play a killing role. Therefore, the artificially modified CAR T Is a potent immune cell that targets...

Claims

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Application Information

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IPC IPC(8): C07H7/033C07H1/00A61K31/7032A61P35/00A61P37/00
Inventor 韩守法刘剑吴选俊林碧娟
Owner XIAMEN UNIV
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