An antigen-linked sialic acid and its application

A sialic acid and antigen technology, applied in the field of sialic acid, can solve the problem that N-acetylmannosamine does not have cell and tissue specificity, etc.

Active Publication Date: 2017-11-14
XIAMEN UNIV
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  • Claims
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Problems solved by technology

However, its disadvantage is that N-acetyl mannosamine has no cell and tissue specificity, and it can be expressed on the cell surface of various tissues of animals 21-23

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  • An antigen-linked sialic acid and its application
  • An antigen-linked sialic acid and its application

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[0057] 1. Laser confocal fluorescence microscopy analysis of DNP-Sia covalently expressed on the surface of cancer cells

[0058]The B16F10 cells were subcultured into 35mm glass-bottomed (NEST) special confocal culture dishes, and cultured in DMEM containing 10% FBS for 24 hours. DNP-Sia (100 μmol / L) was added or not added to these cells, and incubated for 0-24 hours. Incubate with biotinylated Anti-DNP IgG (10 μg / mL) for 15 minutes at 4°C, then stain with phycoerythrin-streptavidin (10 μg / mL) for 15 minutes, and observe DNP-Sia in the cells with a confocal laser fluorescence microscope of the distribution.

[0059] 2. Flow cytometric analysis of DNP-Sia content on the surface of cancer cells

[0060] The B16F10 cells were subcultured into 12-well plates (NEST), and cultured for 24 hours by adding DMEM containing 10% FBS. DNP-Sia (100 μmol / L) was added or not added to these cells, incubated for 0-24 hours, and washed twice with PBS. Incubate with biotinylated Anti-DNP IgG...

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Abstract

An antigen-linked sialic acid and applications thereof relate to sialic acid. The sialic acid linked to the antigen is composed of 2,4-dinitrophenyl and sialic acid, the molecular formula is C17H22N4O12, and the molecular weight is 474.1234. The antigen-linked sialic acid can be used in the preparation of drugs for immunoprevention and treatment of tumors. Antigen-linked sialic acid is covalently expressed on the surface of tumor cells through metabolic pathways after being absorbed by tumor cells; DNP‑Sia is injected intravenously into mice with subcutaneous tumors, and it is quickly and effectively expressed on the surface of tumor cells in solid tumors; Tumor cells expressing DNP-Sia can effectively recruit DNP antibodies or cytotoxic T cells to bind to them, thereby triggering the death of tumor cells. The tumor surface-associated antigens have been expanded, which can effectively regulate the growth of tumors.

Description

technical field [0001] The present invention relates to sialic acid, in particular to an antigen-linked sialic acid and its application. Background technique [0002] The body's immune system destroys "non-self" cells by identifying specific markers on the cell surface, thereby protecting "self" cells. However, "non-me" cancer cells use a variety of ways to evade recognition by the immune system. In order to improve the efficiency of the immune system to kill tumors, a lot of work is in the modification of lymphocytes 1-4 . For example, T cells express chimeric antigen receptors (CAR) through genetic engineering to obtain cytotoxic T cells (CAR T). Since CAR is a fusion protein of the variable region of a single-chain antibody and a T cell signaling molecule, it enables T cells to recognize specific antigens on the surface of tumor cells in a non-MHC-restricted manner and play a killing role. Therefore, the artificially modified CAR T Is a potent immune cell that targets...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H7/033C07H1/00A61K31/7032A61P35/00A61P37/00
Inventor 韩守法刘剑吴选俊林碧娟
Owner XIAMEN UNIV
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