Synchronous aqueous enzymatic ultrasonic extraction for walnut oil and walnut protein peptide

A technology of walnut protein and walnut oil, which is applied in the field of synchronous water-enzyme ultrasonic extraction method, can solve the problems of water-enzyme ultrasonic synergistic extraction, etc., and achieves the effects of shortened processing time, no three-waste pollution, and easy separation.

Inactive Publication Date: 2008-10-08
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, there has been no report on the research and application of water-enzyme ultrasonic co-extraction in the synchronous preparation process of walnut oil and walnut protein peptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Add 9 kg of water to 1 kg of uncoated walnut kernels, colloidally grind, then add papain at a rate of 100,000 units / liter, ultrasonically treat with 1.5 kw for 10 minutes, and continue to heat and stir at 60°C for 30 minutes. After the extraction is completed, centrifuge at 4000 rpm to obtain 0.45 kg of oil phase, 8.5 kg of water phase and 0.5 kg of residue. The obtained oil phase was refined to obtain 0.4 kg of clear walnut oil, the water phase was then concentrated and spray-dried to obtain 0.3 kg of walnut protein peptide mixture, and the residue was dried and ground to obtain 0.3 kg of dietary fiber food.

Embodiment 2

[0017] Example 2 Take 1 kg of undeoiled walnut powder, add 5 kg of water, colloidal grinding, and then add a composite protease composed of bacillus protease + α-amylase at 50,000 protease units per liter, and ultrasonically treat it at 10w for 1 minute, 50 °C, continue to insulate and stir for extraction for 10 minutes. After the extraction is completed, centrifuge at 4000 rpm to obtain 0.5 kg of oil phase, 8.5 kg of water phase and 0.45 kg of residue. The obtained oil phase was refined to obtain 0.45 kg of clear walnut oil, the water phase was concentrated and spray-dried to obtain 0.35 kg of walnut protein peptide mixture, and the residue was dried and ground to obtain 0.2 kg of dietary fiber food.

Embodiment 3

[0018] Example 3 Add 20 kg of water to 1 kg of uncoated walnut kernels, colloidally grind, then add pepsin to 10,000 units per liter, ultrasonicate at 1 kw for 30 minutes, and continue to insulate and stir at 25°C for 180 minutes for extraction. After the extraction is completed, centrifuge at 4000 rpm to obtain 0.45 kg of oil phase, 8.5 kg of water phase and 0.5 kg of residue. The obtained oil phase was refined to obtain 0.4 kg of clear walnut oil, and the water phase was intercepted with a membrane filter to obtain components with molecular weights between 200 and 8000, and then freeze-dried to obtain 0.05 kg of antioxidant peptide mixture derived from walnut protein. kg, other filter components were combined and concentrated and spray-dried to obtain 0.3kg of instant walnut powder, and the residue was dried and ground to obtain 0.2kg of dietary fiber food.

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PUM

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Abstract

The invention relates to an enzyme method ultrasonic extraction method of walnut oil and walnut protein, which belongs to the food and the functional food field. Water is added into walnut kernel or walnut powder to be grinded into paste, protease or compound protease are added into to be performed hydrolization, and simultaneously ultrasonic processing is performed, then the walnut kernel or walnut powder is agitated to perform enzymatic extraction, and then centrifugal separation oil phase, protein peptide oil water phase and residual solid phase are performed; walnut oil is acquired by refining the obtained oil phase, which meets the requirements of green foods; the protein peptide aqueous solution can be directly used to produce degreased walnut protein nutrient milk, or to prepare walnut antioxidation peptide after performed nanofiltration, which is used in health products, food additives, cosmetics or daily chemical articles, or to produce nutrient condensed milk after being performed low temperature concentrating, or to produce walnut protein nutrition powder after performed spray drying; the solid phase residue is prepared into diet fiber food after being dried and grinded into powder; walnut nutrient protein peptide products can be obtained through performing vacuum concentration and spray drying to walnut protein peptide extracting solution containing nutrient content.

Description

technical field [0001] The invention relates to a synchronous hydroenzyme ultrasonic extraction method of walnut oil and walnut protein peptide, belonging to the technical field of food or health food. Background technique [0002] Walnut (Juglans regia L.), also known as Juglans regia L., belongs to Juglans Juglans. Walnut kernels contain up to 65% oil, more than 15.4% protein, in addition to phospholipids, multivitamins, and linoleic acid and linolenic acid in sufficient quantities and appropriate proportions (Table of Food Nutrients, People's Medical Publishing House, 1991). Walnut oil has high medical and edible value (Zhao Shenglan et al., Journal of Yunnan University of Traditional Chinese Medicine, 2008, 1:61), and its preparation methods include pressing, water banding, and solvent extraction. In recent years, the water enzymatic method has been used to extract walnut oil (Yi Jianhua et al.: China Oils and Oils, 2007, 2: 27; 2007, 4: 20), and the clear oil yield can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12P21/06A23J3/14A23C20/02A23L1/305A23L1/36A23L1/308A23C11/10A23L33/18A23L33/22
Inventor 陈朝银赵声兰葛锋韩本勇熊向峰
Owner KUNMING UNIV OF SCI & TECH
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