Mucorales fungi for use in preparation of foodstuffs
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example 7
[0153]Fermentations of the Rhizopus oryzae strain used in Example 2 was scaled up in a pilot plant fermenter with a working volume of 3 m3 from the conditions as described in Example 4. After fermentation the broth was cooled to 5–10° C. and harvested.
[0154]A part of the broth (100 liters) was centrifuged in a Westfalia NA7 disc separator after dilution with tap water to 500 liters. The centrifuge was provided with 4 nozzles, each of a diameter of 1 mm. Two streams of fluid were obtained. The supernatant (400 liters) was discarded and a concentrate stream that contained the biomass (fungal cells) retained. The concentrate was diluted to the original volume with a 100 mM solution of K2HPO4.
[0155]The mixture was recentrifuged and the supernatant was discarded.
[0156]The washed concentrated biomass was then heated to 65° C. for 30 minutes. The concentrated biomass was further heated to 90–95° C. and kept at that temperature for 30 minutes.
[0157]Part of the resultin...
example 8a
Preparation of Edible Biomass
[0158]Three production fermentations the Rhizopus oryzae strain used in Example 2 were performed in a standard production fermenter with a working volume of 30 m3. The fermenter had a pH control, a Rushton turbine with adjustable speed, air supply, foam control and temperature control. At harvest the production microorganisms were killed and the RNA content reduced by heating the biomass to 50–55° C. with direct steam in the presence of 1 g / l benzoic acid at pH 4.5–5.0. After reaching 50–55° C. the broth was cooled to below 20° C. The broth was transferred to another vessel, diluted with cold tap water and further cooled to 4–6° C. The cooled broth was then filtered in a Schenk membrane filter press with a working cake volume of 2.5 m3 and the cake washed with 20 m3 of cold tap water. The cake was squeezed by applying pressurized water in a membrane system (4–9 bar). The cake was discharged from the filter press and part of this crumbled, packed in bags ...
example 8b
Dried Biomass
[0163]The remaining cut particulate biomass for Example 8A was dried in portions or 30–50 kg in a Aeromatic T4 fluid bed dryer with a bottom plate area of 0.26 m2 by means of dry air of a temperature of from 55–65° C. Drying terminated at a bed temperature of 38–40° C. Samples of the dried biomass had the following dry matter contents (Table 8).
[0164]
TABLE 7Fermentation No.Sample No.dry matter content (%)2293.23195.5
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