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Method for preparing silk fibroin/ P(LLA-CL) compound nano fiber structure repair stand

A technology for composite nanofibers and silk fibroin, which is applied in cellulose/protein conjugated artificial filaments, fiber treatment, filament/thread forming, etc., can solve the problems that composite nanofibers have not been reported, and achieve simple preparation methods Ease of operation, abundant raw material resources, and good mechanical properties

Active Publication Date: 2009-08-12
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the method for the composite nanofiber prepared by blending silk fibroin and P(LLA-CL) has not been reported so far

Method used

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  • Method for preparing silk fibroin/ P(LLA-CL) compound nano fiber structure repair stand
  • Method for preparing silk fibroin/ P(LLA-CL) compound nano fiber structure repair stand
  • Method for preparing silk fibroin/ P(LLA-CL) compound nano fiber structure repair stand

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] First add 100 grams of cocoons to 1 liter of 0.5% Na 2 CO 3 Boiling in an aqueous solution for 30 minutes, repeating the treatment three times, fully washing with distilled water, and drying in a drying oven at 45° C. to obtain degummed silk fibers. with CaCl 2:C 2 h 5 OH:H 2 A ternary solvent was prepared with a molar ratio of O=1:2:8, and the silk fiber was hydrolyzed in a water bath at 70°C for 1 hour at a constant temperature at a bath ratio of 1:10 to obtain a completely dissolved brown-yellow silk fiber hydrolysis solution. Put the hydrolyzed solution into a dialysis bag, dialyze with distilled water for 72 hours, put the dialyzed silk fiber hydrolyzed solution into -80°C for 12 hours, and then freeze-dry it at -58°C until dry to obtain a white, odorless, loose porous solid silk fibroin.

[0035] 0.8 gram of silk fibroin was dissolved in 10 milliliters of hexafluoroisopropanol, stirred until completely dissolved to obtain a silk fibroin solution with a conce...

Embodiment 2

[0037] Take by weighing 0.8 grams of silk fibroin prepared in Example 1, dissolve in 10 milliliters of hexafluoroisopropanol, stir until completely dissolved, and obtain a silk fibroin solution with a concentration of 8% (g / ml); P (LLA-CL) is dissolved in 10 milliliters of hexafluoroisopropanols, is stirred until dissolving completely, obtains the P (LLA-CL) solution that concentration is 8% (gram / ml); Two kinds of solutions are with volume ratio 50: 50, the mass ratio of solutes was mixed at 50:50 to obtain a mixed solution with a total concentration of 8%. The mixed solution was subjected to electrospinning, spinning conditions: voltage, 12 kV; electric field distance, 130 mm; spinning rate, 1.2 ml / hour, to obtain a composite nanofiber membrane with an average fiber diameter of 212 nm.

Embodiment 3

[0039] Take by weighing 0.8 grams of silk fibroin prepared in Example 1, dissolve in 10 milliliters of hexafluoroisopropanol, stir until completely dissolved, and obtain a silk fibroin solution with a concentration of 8% (g / ml); P (LLA-CL) is dissolved in 10 milliliters of hexafluoroisopropanols, is stirred until dissolving completely, obtains the P (LLA-CL) solution that concentration is 8% (gram / ml); Two kinds of solutions are with volume ratio 50: 50, the mass ratio of solutes was mixed at 50:50 to obtain a mixed solution with a total concentration of 8%. Electrospinning the mixed solution, spinning conditions: voltage, 12 kV; electric field distance, 130 mm; spinning rate, 1.2 ml / hour, with a grounded roller at 20 cm directly below the needle, and after a certain period of time, take A silk fibroin / P(LLA-CL) composite nanotubular scaffold with a lower internal diameter of 3mm, a wall thickness of 0.2mm, and a length of 5.5cm.

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Abstract

The invention relates to a method for preparing fibroin protein / P(LLA-CL) compound nanofiber tissue repair bracket, comprising : (1) boiling silkworm cocoon with pupa removed in 0.5% of Na2CO3 aqueous solution, then cleaning and drying the boiled silkworm cocoon; then dissolving the boiled silkworm cocoon in ternary solvent, carrying out hydrolysis at constant temperature, dialyzing for 70-72 hours, carrying out prefreezing, then carrying out freezing and drying at the temperature of minus (45-58) DEG C; (2) dissolving the fibroin protein in hexafluoroisopropanol, stirring to dissolve the protein; (3) dissolving the P(LLA-CL) in hexafluoroisopropanol, stirring to dissolve the fiber; (4) mixing the fibroin protein solution with P(LLA-CL) solution, stirring the solution evenly; (5) carrying out electrostatic spinning on blending electrostatic spinning liquid and obtaining the fibroin protein / P(LLA-CL) compound nanofiber. The preparation method of the invention is easy and feasible, the raw materials are abundant and the industrialized production is easy to be realized; in addition, the obtained compound nanofiber features fine biocompatibility, good mechanical property and relatively high porosity factor.

Description

technical field [0001] The invention belongs to the field of preparation of electrospinning composite nanofibers, in particular to a preparation method of silk fibroin / P (LLA-CL) composite nanofiber tissue repair scaffold. Background technique [0002] The development of tissue engineering and regenerative medicine provides an effective treatment for rebuilding or repairing human diseased tissues and organs. Degradable scaffolds provide the necessary space and support for cell growth and tissue regeneration, and provide effective channels for signal transduction, which is one of the key factors that determine the success of tissue engineering. [0003] The design of scaffold materials should maximize the structural and functional characteristics of bionic human extracellular matrix (ECM), in order to effectively solve the problem of biocompatibility of materials, regulate the transmission of biological signals, induce the growth of cells and tissues, and promote the regenera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/56D01F8/02D01F8/14D01D5/00A61L27/22A61L27/18
Inventor 莫秀梅张葵花邱立军王红声
Owner DONGHUA UNIV
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