Method for producing fish scale collagen protein

Abstract

The invention relates to a method for preparing fish scale collagen. The method comprises the following steps: fresh fish scales are taken as a material, undergo cleaning, drying and crushing, are soaked in NaOH solution for degreasing, and soaked in hydrochloric acid solution for deashing; the fresh fish scale is added with water of which the weight is 5 to 10 times of that of the fish scales, the pH value is adjusted to between 2 and 4 by acid solution, pepsin of which the weight accounting for the weight of the mixing solution is between 1 and 5 percent is added, the mixing solution undergoes enzymolysis at a low temperature for 4 to 10 hours, and enzyme is killed; enzymatic liquid is filtered twice to obtain extracting solutions, the extracting solutions are mixed, the mixing solution is decolored, sodium chloride of which the weight accounting for the weight of the mixing solution is between 5 and 15 percent is added into the mixing solution for overnight salting out, deposit is separated and collected, and acetic acid of which the weight accounting for the weight of the mixing solution is between 5 and 20 percent is added into the deposit for dissolution to obtain protein solution; the protein solution is dialyzed and desalted to obtain purified collagen liquid; and the collagen liquid is subjected to freeze-drying or spray-drying and micro-crushing to obtain solid particle. The method has the advantages of reasonable process, simple operation, safety, low cost, little loss of nutrient matters of the product, low ash content and high yield and purity of the collagen.

Description

technical field

[0001] The present invention relates to the preparation of peptide or protein, especially a method for preparing collagen from fish scales. Background technique

[0002] Collagen is an important functional protein with strong biological activity and biological functions. It has been widely used in medicine, food, daily chemical industry, biosynthesis and other industrial fields, such as medicinal capsules, surgical materials, edible Additives, cosmetics, etc. At present, the applied collagen mostly comes from the collagen tissue extracts of mammals or poultry such as cattle, pigs and chickens. In recent years, livestock and poultry diseases such as mad cow disease in cattle, foot-and-mouth disease in pigs and avian influenza in poultry have occurred one after another. The safety of collagen in livestock and poultry has been questioned, and some consumers are allergic to collagen in livestock Propensity. Therefore, the use of collagen extracted from terrest...