Method for producing fish scale collagen protein

A technology of fish scale collagen and collagen, which is applied in the field of peptide or protein preparation, can solve the problems of low yield and purity of fish scale collagen, less nutrient loss, and less ash content of the product, and achieve low cost and less nutrient loss , the effect of simple operation

Inactive Publication Date: 2009-04-29
SHANDONG HOMEY AQUATIC DEV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of the low yield and purity of fish scale collagen in the prior art, a fish scale collagen that can turn waste into treasure, easy to operate, low ash content, high yield and purity, and less nutrient loss is provided. protein preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of fish scale collagen:

[0021] a. Treatment of fish scales Select fresh fish scales to be washed with clean water, dried at 40°C, and then sent to a high-speed tissue grinder for crushing;

[0022] B, degreasing, deashing add the NaOH solution of 5% of fish scale weight to the above-mentioned pulverized fish scales and soak for 56 hours, remove the lipids in the fish scales, wash to neutrality with distilled water; then add 10% of fish scales by weight in hydrochloric acid solution and soak for 24 hours hour, remove the ash in the fish scales, then wash with distilled water until neutral;

[0023] c. Enzymolysis Add the degreased and delimed fish scales to 5 times its weight in water, adjust the pH value to 3 with citric acid, then add pepsin at 2% of the weight of the fish scales, and continuously stir with a magnetic stirrer at a low temperature of 8°C Enzymolysis for 10 hours, after the end of the enzymolysis, the temperature was raised to 95°...

Embodiment 2

[0030] A preparation method of fish scale collagen:

[0031] a. Treatment of fish scales Select fresh fish scales to be washed with clean water, dried at 45°C, and then sent to a high-speed tissue grinder for crushing;

[0032] B, degreasing, deashing add the NaOH solution of 15% of fish scale weight to the above-mentioned pulverized fish scales and soak for 48 hours, remove lipids in the fish scales, wash to neutrality with distilled water; then add 20% of fish scales by weight in hydrochloric acid solution and soak for 12 hour, remove the ash in the fish scales, then wash with distilled water until neutral;

[0033] c. Enzymolysis Add the degreased and delimed fish scales to water 10 times its weight, adjust the pH value to 4 with acetic acid, then add pepsin at 5% by weight of the fish scales, and continuously stir at 4°C for 7 hours for enzymolysis. After enzymatic hydrolysis, the temperature was raised to 90°C and kept for 18 minutes to inactivate the enzyme;

[0034] d...

Embodiment 3

[0040] A preparation method of fish scale collagen:

[0041] a. The treatment of fish scales is to use fresh fish scales to wash with clean water, dry them at a temperature of 50°C, and then send them to a high-speed tissue grinder for crushing;

[0042] b. Degreasing and deliming Add the above pulverized fish scales into NaOH solution with 20% weight of fish scales and soak for 24 hours, remove lipids in fish scales, wash with distilled water until neutral; then add 5% weight of fish scales with hydrochloric acid solution and soak for 36 hours hour, remove the ash in the fish scales, then wash with distilled water until neutral;

[0043]c. Enzymolysis Add the above-mentioned degreased and deashed fish scales to 8 times its weight in water, adjust the pH value to 2 with citric acid, then add pepsin with 2.5% of the weight of the fish scales, and use a magnetic stirrer to continuously stir at a low temperature of 6°C Enzyme hydrolysis for 5 hours, after the end of the enzymati...

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Abstract

The invention relates to a method for preparing fish scale collagen. The method comprises the following steps: fresh fish scales are taken as a material, undergo cleaning, drying and crushing, are soaked in NaOH solution for degreasing, and soaked in hydrochloric acid solution for deashing; the fresh fish scale is added with water of which the weight is 5 to 10 times of that of the fish scales, the pH value is adjusted to between 2 and 4 by acid solution, pepsin of which the weight accounting for the weight of the mixing solution is between 1 and 5 percent is added, the mixing solution undergoes enzymolysis at a low temperature for 4 to 10 hours, and enzyme is killed; enzymatic liquid is filtered twice to obtain extracting solutions, the extracting solutions are mixed, the mixing solution is decolored, sodium chloride of which the weight accounting for the weight of the mixing solution is between 5 and 15 percent is added into the mixing solution for overnight salting out, deposit is separated and collected, and acetic acid of which the weight accounting for the weight of the mixing solution is between 5 and 20 percent is added into the deposit for dissolution to obtain protein solution; the protein solution is dialyzed and desalted to obtain purified collagen liquid; and the collagen liquid is subjected to freeze-drying or spray-drying and micro-crushing to obtain solid particle. The method has the advantages of reasonable process, simple operation, safety, low cost, little loss of nutrient matters of the product, low ash content and high yield and purity of the collagen.

Description

technical field [0001] The present invention relates to the preparation of peptide or protein, especially a method for preparing collagen from fish scales. Background technique [0002] Collagen is an important functional protein with strong biological activity and biological functions. It has been widely used in medicine, food, daily chemical industry, biosynthesis and other industrial fields, such as medicinal capsules, surgical materials, edible Additives, cosmetics, etc. At present, the applied collagen mostly comes from the collagen tissue extracts of mammals or poultry such as cattle, pigs and chickens. In recent years, livestock and poultry diseases such as mad cow disease in cattle, foot-and-mouth disease in pigs and avian influenza in poultry have occurred one after another. The safety of collagen in livestock and poultry has been questioned, and some consumers are allergic to collagen in livestock Propensity. Therefore, the use of collagen extracted from terrest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K14/78
Inventor 夏雪奎唐聚德袁文鹏胡炜王小军孙永军孟秀梅张绵松刘昌衡
Owner SHANDONG HOMEY AQUATIC DEV
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