Method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of pregnant woman

Abstract

The invention discloses a method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of a pregnant woman. The method comprises the following steps: 1, preprocessing fresh pregnant woman maternal peripheral blood: separating and enriching monocytes containing fetal nucleated red blood cells from maternal peripheral whole blood through a density gradient centrifugation technology; 2, capturing cells: adding a separated monocyte suspension to a substrate chip modified with an antibody, and carrying out standing capturing; and 3, identifying the sex of a fetus: identifying whether the nucleated red blood cells of the captured fetus contain Y chromosome or not by using an FISH probe technology (CSP-X / Y). The method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of the pregnant woman can realize capture and identification of the fetal nucleated red blood cells from the maternal peripheral circulating blood, and allows the FISH analysis to be directly carried out in order to realize noninvasive antenatal diagnosis of the sex or 21-Trisomy syndrome and other chromosome diseases of the fetus.

Description

Technical field: [0001] The present invention involves a method of non -invasive prenatal diagnosis, and especially involves a method that uses nano -materials to capture the fetus from the peripheral blood of pregnant women with nuclear red blood cells for non -invasive prenatal diagnosis. Background technique: [0002] Preparatory diagnosis, also known as diagnosis or intrauterine diagnosis before birth, refers to the application of modern biology, biochemical, immunogenic genetic, cell genetic, molecular geneticism and other technologies before the fetus is born.Detection to understand the appearance of the fetus, perform nuclear analysis of the fetal chromosomes, detect the biochemical component of the fetal cells, or generate a genetic analysis of it, so as to make a timely diagnosis of congenital malformations or hereditary diseases of some fetuses to prevent seriousnessThe birth of genetic diseases, intellectual disorders, and congenital malformed fetuses.Therefore, prena...

AI Technical Summary

Problems solved by technology

However, this kind of examination belongs to the morphological level, and can only detect whether the fetus is deformed, the diagnostic range is limited, and the sensitivity and accuracy of the diagnostic method are poor

Amniocentesis is the main method of invasive prenatal diagnosis. Amniocentesis requires the use of a long needle with a core to enter the amniotic cavity of pregnant women to extract amniotic fluid multiple times. This method is invasive and has certain risks for both the fetus and the pregnant woman. It is particularly easy to cause adverse complications such as intrauterine infection, miscarriage, and fetal death, and is not easily accepted by pregnant women and their families

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