30 results about "Antepartum diagnosis" patented technology

The invention discloses a single-tube amplification kit for simultaneously detecting alpha and beta thalassemia genes, and the kit comprises (1) a gene chip on which a probe is arranged as SEQID No:1-37 and its complementary sequence; (2) one group of primers which is used for multiple PCR (polymerase chain reaction) and regarded as SEDID No. 38-46. Through the kit, alpha and beta thalassemia genes can be simultaneously amplified in the same reaction tube, and three deleted alpha-thalassemia genes (SEA, -alpha 3.7 and -alpha 4.2), three mutant alpha-thalassemia genes (CS, QS and WS) and 19 mutant beta-thalassemia genes can be simultaneously detected. In the same reaction tube, alpha-thalassemia genes and beta-thalassemia genes can be also simultaneously amplified, thereby greatly improving the diagnostic efficiency and accuracy, reducing the cost and shortening he detection time, thus the kit has the great significance of thalassemia crowd screening, genetic counseling and antenatal diagnosis.

The invention discloses a method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of a pregnant woman. The method comprises the following steps: 1, preprocessing fresh pregnant woman maternal peripheral blood: separating and enriching monocytes containing fetal nucleated red blood cells from maternal peripheral whole blood through a density gradient centrifugation technology; 2, capturing cells: adding a separated monocyte suspension to a substrate chip modified with an antibody, and carrying out standing capturing; and 3, identifying the sex of a fetus: identifying whether the nucleated red blood cells of the captured fetus contain Y chromosome or not by using an FISH probe technology (CSP-X / Y). The method for noninvasive antenatal diagnosis through separating fetal nucleated red blood cells from peripheral circulating blood of the pregnant woman can realize capture and identification of the fetal nucleated red blood cells from the maternal peripheral circulating blood, and allows the FISH analysis to be directly carried out in order to realize noninvasive antenatal diagnosis of the sex or 21-Trisomy syndrome and other chromosome diseases of the fetus.

The invention belongs to the field of gene sequencing, and discloses an establishment method for a small fragmental DNA (Deoxyribose Nucleic Acid) library based on an Ion ProtonTM sequencing platform, and a relevant solvent composition solution. The establishment method disclosed by the invention comprises the steps of DNA sample extraction, tail end repairing, fragment selection, joint connection, PCR (Polymerase Chain Reaction) amplification, library detection, high-throughput sequencing and the like, wherein a reagent I, a reagent II and a reagent III are respectively adopted in the steps of tail end repairing, joint connection and PCR amplification. The establishment method disclosed by the invention is precise and simple in step, simple to operate, small in reagent variety and low in cost, the loss and waste of the library are reduced, and the working efficiency is improved; in addition, the establishment method can be applied to noninvasive antenatal diagnosis on pregnant woman peripheral blood fetus chromosome aneuploid based on the Ion ProtonTM sequencing platform, the diagnosis result is high in accuracy rate and is safe and economic, and the birth rate of chromosome aneuploid fetuses can be effectively controlled.

The invention discloses an assistance device for amniocentesis for antenatal diagnosis, and effectively solves the problem that operation is inconvenient when a medical worker conducts amniocentesis. The assistance device comprises a supporting plate; a guide groove is formed in the supporting plate; a semi-annular groove is formed in the supporting plate; a rotating cylinder is rotationally connected in the supporting plate; a spiral groove is formed in the rotating cylinder; a supporting column is coaxially arranged in the rotating cylinder; a guide column is arranged at the lower side of the supporting column; a fluid pumping device is detachably connected to the left end of the supporting plate; the fluid pumping device comprises a fixed cylinder; the lower end of the fixed cylinder is provided with an end cover; a waste fluid cylinder is connected to the lower end of the end cover in a sliding manner; the lower end of the waste fluid cylinder is provided with a primary teat; a lower piston is connected in the waste fluid cylinder in a sliding manner; an upper piston is detachably connected to the upper end of the lower piston; a main piston is detachably connected to the upper end of the upper piston; a connecting pipe is fixed onto the upper piston; and a secondary teat is fixed to the lower end of a secondary piston. The assistance device is novel in structure and high in usability, reduces steps when the medical worker conducts the amniocentesis, increases the working efficiency of the medical worker, and is conducive to use of the medical worker.

The invention discloses a portable quick antenatal diagnosis device, and relates to the technical field of medical instruments, the portable quick antenatal diagnosis device comprises a fixing plate, two sliding sleeves are clamped in the fixing plate, two sliding rods are slidably connected in the sliding sleeves, and the opposite ends of the two sliding rods are fixedly connected with the opposite surfaces of two first supporting plates respectively. According to the convenient-to-carry quick antenatal diagnosis device, a screw rod is rotated through a first handle, two threaded sleeves can be made to get close to each other, the threaded sleeves can drive a front supporting plate and a rear supporting plate to get away from each other in the moving process of the threaded sleeves through cooperation with a first ear plate, a second ear plate, a connecting rod, a sliding sleeve and a sliding rod, then the device is pushed through a second handle, and the device is convenient to carry. A sickbed where a pregnant woman lies is placed between the two first supporting plates, then the diagnostic apparatus body is opened, the abdomen of the pregnant woman can be scanned through the B-ultrasonic probe, when the pregnant woman needs to be subjected to antenatal examination, the pregnant woman does not need to get up to go to an examination room, and therefore the trouble of antenatal examination of the pregnant woman is solved.

The invention belongs to the technical field of biology and particularly relates to a primer set and a kit for detecting rare deletion type thalassemia. The primer set and the kit can be directly used for rapidly and stably detecting domestic 11 types of known rare deletion type thalassemia. The primer set for detecting the rare deletion type thalassemia comprises a primer set A and a primer set B, wherein the primer set A comprises 12 primers for detecting alpha-thalassemia deletion gene types; the primer set B comprises 10 primers for detecting beta-thalassemia deletion gene types. The kit can adopt a multiplex Gap-PCR (Polymerase Chain Reaction) technology to detect 11 rare deletion gene types and can be used for directly detecting 11 rare deletion thalassemia gene types for one time. The kit is simple to operate and saves time and cost when being compared with a manner of combining a nested PCR with a genetic analysis in antenatal diagnosis; conditions are created for comprehensively carrying out thalassemia screening and scientific evidences are provided for thalassemia diagnosis of premarital detection, antenatal detection and fetuses of the pregnancy.

The clinical antenatal diagnosis and examination device comprises an operating table, and sliding grooves are formed in the left side and the right side of the operating table in the front-back direction; the number of the first screw rods is two, the rear ends of the two first screw rods are rotatably arranged on the rear side of the inner cavity of the sliding groove through bearings, and the front ends of the first screw rods extend out of the front side of the operating table; the belt pulley sleeves the front side of the outer wall of the first screw rod; the two ends of the belt are connected to the outer walls of the two belt wheels in a sleeving mode correspondingly; the rear end of the rocker is arranged at the front end of the first screw on the right side; the sliding block is inserted into the middle of an inner cavity of the sliding groove in a matched mode, and the sliding block is connected to the middle of the outer wall of the first screw in a threaded mode. The device can prevent the puncture needle from swinging greatly when the needle tube is inserted and pulled out, further can prevent a fetus from being accidentally injured, eliminates potential safety hazards, and is convenient to use.

Disclosed are a preserving solution for a maternal peripheral blood sample and method for preserving the maternal peripheral blood sample. The preserving solution of the present invention consists of an Alsever solution and an apoptosis inhibitor at a volume ratio of 5-15:0.03-0.1. The preserving solution of the present invention utilizes the Alsever solution and the apoptosis inhibitor for the preservation of maternal peripheral blood, thus effectively delaying apoptosis in the maternal peripheral blood and maintaining various indexes of a cell to keep the cell fresh as far as possible; maintaining cell morphology, providing nutrients for the cell and maintaining various physicochemical properties of the cell; providing a basis for the gradient centrifugation and flow sorting of subsequent nucleated red blood cells and other rare cells, and ensuring preservation of a large amount of fresh nucleated red blood cells and accurate non-invasive antenatal diagnosis.

The invention provides a clinical antenatal diagnosis and examination device based on the obstetrics and gynecology department, and relates to the technical field of medical instruments. The control part is arranged above the supporting part; the limiting part is arranged on the front side of the supporting part; the leg supporting part is arranged on the right side of the supporting part; the auxiliary part is arranged below the leg supporting part; a supporting block is fixed to the right end of a cylindrical sliding rod, so that the stability of the cylindrical sliding rod is kept, a rotating frame is fixed to the bottom of a leg supporting plate through a screw, so that the rotating frame conveniently moves along with the leg supporting plate, and a mounting frame is conveniently driven to approach a pregnant woman through a connecting plate; by means of the arrangement, detection tools can be conveniently placed and installed through the installation frame, a doctor is prevented from getting up for many times to take the detection tools, the situation that the detection time is long is avoided, and the problems that most pregnant women directly lie on a sickbed and open the two legs for detection, the legs of the pregnant women are inconvenient to adjust in the pregnancy period, and consequently the pregnant women cannot support the legs for a long time are solved.

The invention relates to a kit for detecting the STR (Short Tandem Repeat) genetype of human chromosomes 21, 18 and 13 and sex chromosomes, and particularly relates to a QF-PCR (Quantitative Fluorescence-Polymerase Chain Reaction) kit for detecting the number of the chromosomes 21, 18 and 13 and the sex chromosomes by adopting five-color fluorescence labeling monotube fast multiple amplification and mainly for diagnosing 21 trisomy syndrome, 18 trisomy syndrome, 13 trisomy syndrome and the aneuploid abnormality of the sex chromosomes. The kit comprises a primer mixture, a hot start C-Taq enzyme, an amplification reaction solution, a positive quality control product, a negative reference product, a fluorescence interior label Siz-500 and an allelic gene typing standard substance. Compared with the traditional antenatal diagnosis method, the kit disclosed by the invention can realize high-flux, fast, reliable and standardized detection.

The clinical antenatal diagnosis and examination device comprises a shell, a puncture needle and a sample collection needle cylinder, an ultrasonic probe is installed in the shell, a driving motor is installed at the lower end of the shell, a sliding groove is formed in the position, above the driving motor, of the shell, and a threaded shaft connected with the driving motor is rotationally connected into the sliding groove; a magnet sliding block is slidably connected into the sliding groove, and a threaded hole which is in threaded connection with the threaded shaft downwards penetrates through the magnet sliding block. A sliding rail is arranged on the outer side, close to the sliding groove, of the shell and slidably connected with a sliding iron sheet matched with the magnet sliding block, the sliding iron sheet is outwards and horizontally provided with a U-shaped clamp used for clamping a puncture needle, a puncture guide block is arranged at the position, at the lower end of the sliding rail, of the shell, and a guide hole for the puncture needle to penetrate through is formed in the position, at the lower end of the sliding rail, of the shell and penetrates through the puncture guide block downwards. And one end of the sample collection needle cylinder is detachably connected with the puncture needle. Ultrasonic imaging can be combined for automatic puncture, the puncture depth is automatically judged according to ultrasonic waves, and the puncture depth is more accurate.

The invention relates to the technical field of antenatal diagnosis devices, and discloses an auxiliary device for antenatal diagnosis nursing, which comprises a supporting plate, the upper part of the supporting plate is fixedly connected with a bottom limiting frame, the lower part of the bottom limiting frame is provided with a first motor, and the upper part of the first motor is provided with a telescopic device; and the upper part of the telescopic device is fixedly connected with a lying bed. According to the auxiliary device for antenatal diagnosis and nursing, a second motor is started to drive a gear installed on the lower portion of the second motor and a gear belt to do threaded motion, and then a first threaded rod can be driven to rotate, so that the inclination angle of a threaded sleeve is driven to be adjusted, and then the angle of a backrest is driven to be adjusted; and meanwhile, the synchronism is increased when the two first threaded rods are driven to rotate, and when the angle of the connecting plate is adjusted, the second motor and the connecting plate can synchronously move, so that the influence on the position of the second motor when the connecting plate moves is reduced.

The invention provides a clinical antenatal diagnosis and examination device for the obstetrics and gynecology department, and relates to the technical field of examination devices. The mounting mechanism comprises overturning pieces and baffles, the overturning pieces are arranged on the two sides of the main body in the mounting mechanism, two sets of gears at the upper ends of the overturning pieces are rotationally mounted on the main body, and T-shaped grooves are formed in the overturning pieces. The racks on the two sides of the bearing plate are clamped with the gears on the overturning piece, the overturning piece can be driven to rotate while the bearing plate descends, the baffle is overturned to the upper end from the bottom of the main body, and through the arrangement, when a pregnant woman sits on the bearing plate, the baffle is automatically overturned and shields the pregnant woman so as to prevent the pregnant woman from accidental exposure; the problems that an existing diagnostic examination device is poor in privacy, a pregnant woman needs to be exposed to a certain degree in the examination process, and if the pregnant woman cannot be shielded in time, the pregnant woman feels embarrassed are solved.

The invention discloses a method for detecting the fetus DNA content of pregnant woman blood plasma. The method comprises the following steps: simultaneously carrying out multiple fluorescence PCR amplification and product capillary electrophoresis separation on multiple SNP sites in the genome and the plasma of a pregnant woman through multiple fluorescence PCR amplification and capillary electrophoresis separation based on insertion / deletion SNP sites which are different in mother genome and fetus genome; selecting SNPs homozygous in the maternal genome and heterozygous in the fetus, carrying out peak height correction on the obtained capillary electrophoresis detection result by using a reference sample, and correcting the detection background by using the maternal genome DNA in order to quantitatively analyze two allele fragments of many SNP sites in the blood plasma DNA and obtain the proportion of the fetus DNA in the mother blood plasma DNA. The method has the advantages of simplicity, fastness, low cost and high accuracy when used in high-flux detection of the multiple SNP sites, and has wide application prospect in the field of noninvasive antenatal diagnosis.