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Composite amplification detection system for linked STR gene loci on human chromosomes I and II and application of composite amplification detection system

A technology of chromosome 2 and detection system, which is applied in the field of complex amplification detection system for linked STR loci on human chromosome 1 and 2, which can solve the problems that STR kits are not tightly linked STRs and have not been reported, and achieve mutation The effect of low rate, good stability and high individual recognition rate

Pending Publication Date: 2022-04-29
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, except for the X chromosome STR locus, none of the existing STR kits used in forensic identification are tightly linked STRs, and there has been no report of a tightly linked STR multiplex amplification system that can be applied to actual kinship identification.

Method used

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  • Composite amplification detection system for linked STR gene loci on human chromosomes I and II and application of composite amplification detection system
  • Composite amplification detection system for linked STR gene loci on human chromosomes I and II and application of composite amplification detection system
  • Composite amplification detection system for linked STR gene loci on human chromosomes I and II and application of composite amplification detection system

Examples

Experimental program
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Effect test

Embodiment 1

[0119] Example 1 Application of two sets of fluorescence-labeled multiple amplification systems containing 28 linked AS-STR loci for intergenerational relative identification

[0120] 1. Principle

[0121] In the human genome, there is a class of closely linked STR loci, and the linked STR constituting haplotye is inherited from parent to offspring, and the closely linked STR haplotype has a high degree of polymorphism. The probability of having the same haplotype between unrelated individuals is very small, which can overcome the low polymorphism of conventional non-linked STRs and the interference of genetic background, and break through the current problem of not being able to identify second-order or higher kinship relationships and distinguish different kinship relationships. If the polymorphism of a closely linked STR group haplotype is infinitely high, then the probability of having the same haplotype between unrelated individuals will be very small, and only related in...

Embodiment 2

[0143] A total of 25 members of the four-generation family were detected by the linked STR complex amplification system of the present invention, and it was confirmed that the linked group of linked STRs was inherited according to the haplotype. The specific identification process adopts the operation steps of Example 1: DNA extraction—PCR amplification—electrophoresis of PCR amplification products—result analysis.

[0144] The result is parsed as Figure 5 shown, from Figure 5 It can be seen that 13 linked STR loci on chromosome 2 constitute 4 linkage groups (Ⅰ: D2S2696-D2S410-D2S437-D2S2970; Ⅱ: D2S1339-D2S1399; Ⅲ: D2S2978-D2S1361-D2S1391; ) is stably inherited in three or four generations.

[0145] in Figure 5 Individual 25 in linkage group Ⅰ: D2S2696-D2S410-D2S437-D2S2970; Ⅱ: D2S1339-D2S1399; Ⅲ: D2S2978-D2S1361-D2S1391 haplotype from great-grandfather; Ⅳ: D2S1371-D2S434-D2S1338-D2S427 haplotype from great grandmother.

[0146] The 28 loci of the present invention have b...

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Abstract

The invention relates to the technical field of nucleic acid detection, in particular to a multiplex amplification detection system for linked STR loci on human chromosomes I and II and application of the multiplex amplification detection system. According to the invention, closely linked STR gene loci with high polymorphism are respectively screened from chromosomes 1 and 2, and two groups of composite amplification systems are established. The closely linked STR composite amplification detection system developed by the invention is not only suitable for genetic identification, but also can be used for distinguishing a single parent diploid with multi-site mutation or excluding a genetic relationship, is used for antenatal diagnosis of chromosome abnormality, and has a wide market application prospect.

Description

technical field [0001] The invention relates to the technical field of nucleic acid detection, in particular to a complex amplification detection system for linked STR loci on human No. 1 and No. 2 chromosomes and its application. Background technique [0002] Family identification is a difficult point in forensic identification. The identification of unknown persons, identification of relatives of lost persons, claim of persons who died in accidents or traffic accidents, and even identification of the origin of victims of catastrophic events will involve relative identification. For some parentage determinations without parental involvement, except for Y chromosome and mitochondrial DNA genetic markers that can exclude paternal or maternal relationship, when using non-linked genetic markers on autosomal, due to the inability to directly observe Inheritance of alleles, kinship cannot be ruled out directly, and kinship can only be assessed by comparing the probability of all...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6883C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6883C12Q1/6858C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 刘秋玲吕德坚赵虎杨建广黄斌
Owner SUN YAT SEN UNIV
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