Construction method and application of small fragment DNA library based on ion ProtonTM sequencing platform
A DNA library and sequencing platform technology, applied in the field of gene sequencing and high-throughput sequencing, can solve the problems of waste, increased cost, and cumbersome construction of small-fragment DNA libraries.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0102] Embodiment 1: Based on IonProton TM Method for constructing small fragment DNA library of sequencing platform
[0103] 1. Reagents
[0104]This embodiment uses the following reagents: EndRepairEnzyme (LifeTechnology), DNALigase (LifeTechnology), NickRepairPolymerase (LifeTechnology), Adapter (LifeTechnology), BarcodeX (LifeTechnology), lowTEbuffer (10mMTris-HCl, 0.1mMEDTA, pH 8.0);
[0105] The pH value of reagent I is 7.5, the solvent is water, and the solute is: 10mM Tris-HCl, 100mM KCl, 2mM dithiothreitol, 10mMATP, 10mMdNTPs mixture, 6U / μl EndRepair enzyme;
[0106] The pH value of reagent II is 7.6, the solvent is water, and the solute is: 50mM Tris-HCl, 10mMMgCl 2 , 10mM dithiothreitol, 1mMATP, 2mMdNTPs mixture, 400U / μl DNA ligase and 10U / μl NickRepair enzyme;
[0107] The pH value of reagent III is 7.6, the solvent is water, and the solute is: 66mMTris-SO 4 (pH8.9), 19.8mM (NH 4 ) 2 SO 4 , 2.4mMMgSO 4 , 220μM dNTPs mixture, 22U / ml recombinant TaqDNApolymer...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com