Single-tube amplification kit for simultaneously detecting alpha and beta thalassemia genes

Abstract

The invention discloses a single-tube amplification kit for simultaneously detecting alpha and beta thalassemia genes, and the kit comprises (1) a gene chip on which a probe is arranged as SEQID No:1-37 and its complementary sequence; (2) one group of primers which is used for multiple PCR (polymerase chain reaction) and regarded as SEDID No. 38-46. Through the kit, alpha and beta thalassemia genes can be simultaneously amplified in the same reaction tube, and three deleted alpha-thalassemia genes (SEA, -alpha 3.7 and -alpha 4.2), three mutant alpha-thalassemia genes (CS, QS and WS) and 19 mutant beta-thalassemia genes can be simultaneously detected. In the same reaction tube, alpha-thalassemia genes and beta-thalassemia genes can be also simultaneously amplified, thereby greatly improving the diagnostic efficiency and accuracy, reducing the cost and shortening he detection time, thus the kit has the great significance of thalassemia crowd screening, genetic counseling and antenatal diagnosis.

Description

technical field [0001] The invention relates to the field of genes, in particular to a single-tube amplification and simultaneous detection of α and β thalassemia gene kits. Background technique [0002] Thalassemia (referred to as thalassemia) is a hemolytic anemia caused by an imbalance in the synthesis rate of α, β-globin peptide chains due to human gene mutation or deletion. Thalassemia is one of the most common genetic diseases in the world, and it is widely distributed in Mediterranean countries and other areas where malaria used to be high. In my country, the provinces south of the Yangtze River have a high incidence rate. In addition to Guangdong, Guangxi, and Hainan provinces with the highest incidence rates, Guizhou, Yunnan, and Sichuan are also high-incidence areas. The two common types of thalassemia are alpha-thalassemia and beta-thalassemia. [0003] α-thalassemia is a chronic hemolytic disease caused by autosomal genetic defect, which causes the loss or dysf...

AI Technical Summary

Problems solved by technology

These products have high operating requirements, cumbersome steps, and long time-consuming, which cannot meet the simple and fast requirements of clinical diagnosis.

[0010] So far, there is no product that can be used to amplify α-thalassemia and β-thalassemia genes in the same reaction tube in clinical use for detection of thalassemia.

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