Supercharge Your Innovation With Domain-Expert AI Agents!

Method for detecting abnormal numbers of five chromosomes

A chromosome number and chromosome technology, applied in the field of detection, can solve the problems of high technical requirements, high fees, and long time consumption, and achieve the effects of low cost, short time consumption, high sensitivity and accuracy

Active Publication Date: 2013-05-01
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
View PDF3 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of karyotype analysis is that it takes a long time (more than a week), relatively high technical requirements, and high fees

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting abnormal numbers of five chromosomes
  • Method for detecting abnormal numbers of five chromosomes
  • Method for detecting abnormal numbers of five chromosomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 4

[0068] figure 1 Case analysis of chromosomal aneuploidy. The results of GeXP capillary electrophoresis analysis of the DNA samples (50-100ng) shown in the figure are from top to bottom: normal female DNA sample; monosomy of chromosome X; trisomy of chromosome 13; trisomy of chromosome 18 and chromosomal 21 Chromosome trisomy. Chromosomal aneuploidy can be detected by quantitative analysis. For example, by observing the relative height of the specific peaks of each chromosome in the black box, it can be clearly shown that the difference in peak height corresponds to chromosome aneuploidy or sex; in addition, by comparing and calculating the relative peak area of ​​each specific peak of each chromosome, the chromosome can be more accurately displayed Aneuploidy (see "Example 4, Data Analysis")

[0069] figure 2 Quantitative analysis of chromosome number in normal samples. The relative copy number of each gene (primer pair) on each chromosome (chromosomes 13, 18 and 21) rep...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a method for detecting abnormal numbers of five chromosomes, wherein the method is provided for concurrently detecting aneuploidy of numbers of five chromosomes such as chromosome 21, chromosome 13, chromosome 18, chromosome X and chromosome Y, and comprises: (1) respectively selecting 7 genes from the five chromosomes, and designing 38 pairs of primers based on the selected genes; (2) adopting combinations of the primers to carry out multiplex PCR and capillary electrophoresis; and (3) adopting a software to analyze electrophoresis results.

Description

technical field [0001] The invention relates to a detection method, in particular, the invention relates to a method for detection of abnormal number of five chromosomes. Background technique [0002] Trisomy 21, also known as Down syndrome or congenital stupidity, is an autosomal aberration and is the most common type of chromosomal disease in children. The incidence rate among live babies is about 1 / (600-800). The higher the incidence of the disease. About 60% of the children died in early fetal abortion. Trisomy 21 can cause learning disabilities, intellectual disabilities, and disabilities. In addition, aneuploidies of chromosomes 13, 18, X, and Y lead to Patau syndrome, Edwards syndrome, Klinefelter syndrome, and Turner syndrome, respectively. The disease can cause abnormal development and other pathological changes in the human body to varying degrees. Once a child is born, it will bring great pain to the family and a heavy burden to the society. [0003] At prese...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 吴勇徐冰
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com