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A method and application for realizing animal sex control based on rbmy gene editing

A sex and gene technology, applied in the field of genetic engineering, can solve problems such as increasing the birth ratio of female offspring, reducing the number of gene copies, and male sterility

Active Publication Date: 2020-01-31
SOUTH CHINA AGRI UNIV
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Problems solved by technology

Most of these genes are only expressed in the germline of male mice and are closely related to spermatogenesis. Knocking out part of the long arm of the Y chromosome will reduce the copy number of this part of the gene, resulting in sperm deformities and even male sterility, while increasing the number of females. Birth ratio of offspring

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  • A method and application for realizing animal sex control based on rbmy gene editing
  • A method and application for realizing animal sex control based on rbmy gene editing
  • A method and application for realizing animal sex control based on rbmy gene editing

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Embodiment Construction

[0038] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0039] pU6-sgRNA empty vector, pT7-sgRNA empty vector, pT7-Cas9 empty vector, phCas9 vector and pGCS empty vector were obtained from the Guangzhou Center for Medicine and Health, Chinese Academy of Sciences; pEGFP empty vector gene sequence was obtained from GenBank accession number: U55762.1, the vector Purchased from Yubao Bioengineering (Dalian) Co., Ltd.

[0040] 1. Application of CRISPR / Cas9 system to cut mouse Y chromosome target site selection

[0041] According to the prediction of the online software (https: / / chopchop.rc.fas.harvard.edu / index.php) combined with the recognition characteristics of sgRNA and the least off-target probability, this experiment selected 6 targets of the Y chromosome multi-copy gene Rbmy The specific information of the target site is shown in Table 1 below.

[0042]

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Abstract

The invention discloses an animal sex control method based on Rbmy gene editing and application. The method controls animal sex by using CRISPR / Cas9 system to specifically cut Rbmy gene on Y chromosome. Rbmy gene encodes a germ cell specific nucleoprotein, this protein is an important factor related to spermatogenesis, and by cutting Rbmy gene, it is possible to deactivate Rbmy gene, thus Y sperm or fertilized ovum with Y sperm is deactivated and cannot develop into a normal embryo, birth ratio of female animals is finally increased and sex control is achieved. Therefore, sex ratio of animal offspring can be controlled through the method so that the ratio of female animals with better economic value is increased and production efficiency and economic effect are greatly improved.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method and application for realizing sex control of animals based on editing the Rbmy gene. Background technique [0002] Sex control refers to a technology that artificially intervenes in the reproductive process of animals so that female animals can produce offspring that meet a specific sex. Animal sex control technology is of great significance in preventing human genetic diseases, improving livestock production capacity, animal genetic breeding and protection of endangered and rare animals. [0003] At present, there are more than 300 kinds of sex-linked genetic diseases related to the sex of the baby at birth (such as hemophilia, red-green color blindness and hereditary nephritis, etc.), the use of sex control technology in early pregnancy can predict the occurrence of sex-linked genetic diseases, So as to achieve the purpose of prenatal diagnosis and prevent...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/877
CPCC07K14/47C12N15/8509C12N15/877C12N2800/107C12N2800/80C12N2810/10
Inventor 吴珍芳李紫聪杨晓峰李崇刘德武蔡更元
Owner SOUTH CHINA AGRI UNIV
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