Earwax collection and detection device and method

Abstract

The present invention discloses an earwax collection and detection device and method. The device includes an earwax collection device, an air intake device and a post-stage detection system; the earwax collection device includes a spoon-shaped front end, a sensor, a rod, a sleeve, a pressure plate, a spring, a pipe cap, a temperature control sheet, a micro battery and a collection control circuit; the air intake device includes an air intake pipe and a filter; the post-stage detection system includes an inlet, an adsorption tube, a clustered capillary column, a surface acoustic wave sensor, a six-way valve, a carrier gas bottle, a vacuum pump, a flow meter and a detection control circuit; after the earwax collection device completes earwax collection using a spoon-shaped front end, the spoon-shaped front end is placed in the sleeve for sealed storage and cold chain transportation. In conjunction with the air intake device and the post-stage detection system, the collected earwax sample can be tested for volatile organic compounds. The present invention provides an integrated and efficient solution for the collection, storage, cold chain transportation and detection of earwax in the ear canal.

Description

Technical Field

[0001] The present invention relates to the field of volatile matter collection and detection, and in particular to an ear canal cerumen collection and detection device and method. Background Art

[0002] In the past two decades, the analysis of volatile organic compounds (VOCs) for clinical disease diagnosis has attracted widespread attention. Earwax is considered to be a mixture of special sweat gland secretions and sebaceous gland fat substances, which has great similarities with the composition of sweat. The main volatile organic compounds (VOCs) in normal earwax include ketones, alcohols, dimethyl disulfide, alkyl furans, pyrroles, fatty acids, minerals, steroids, pyrazines, and some straight-chain and branched hydrocarbons. Any changes in homeostasis caused by inherited metabolic disorders or infections will lead to changes in the composition and quantity of volatile organic compounds (VOCs) secreted by the skin or in sweat. Therefore, the analysis of volatile organic compounds (VOCs) in earwax can be used to judge the health status of an individual. However, there is currently no integrated and efficient solution for the collection, storage, cold chain transportation and detection of earwax. Summary of the invention

[0003] The present invention proposes an ear canal cerumen collection and detection device and method, providing an integrated solution for the collection, storage, cold chain transportation and detection of ear canal cerumen, and providing a non-invasive and standardized collection and detection solution for the diagnosis of related clinical diseases such as diabetes, Parkinson's disease, etc.

[0004] The objective of the present invention is achieved through the following technical solutions:

[0005] According to a first aspect of the present specification, there is provided an earwax collection and detection device, comprising an earwax collection device, an air intake device and a post-stage detection system;

[0006] The earwax collection device includes a spoon-shaped front end, a sensor, a rod, a sleeve, a pressure plate, a spring, a tube cap, a temperature control sheet, a micro battery and a collection control circuit; the sensor is a QCM mass sensitive sensor, which is built into the spoon-shaped front end and is used to obtain the mass of the collected earwax; the sleeve is a hollow tube, the spoon-shaped front end and the rod are connected together, and the earwax is pushed out of the sleeve when collecting, and is pushed into the sleeve after the collection is completed; the pressure plate and the spring are the internal structure of the sleeve front end, the upper end of the spring is connected to the sleeve, and the lower end is connected to the pressure plate, and the pressure plate can move up and down along the slide structure under the action of the spring elastic force or external force; in the process of pushing the spoon-shaped front end and the rod into the sleeve, under the action of the spring elastic force, the pressure plate will gradually flatten and compact the earwax on the spoon-shaped front end; the collection control circuit controls the temperature control sheet arranged inside the front end of the sleeve by adopting the PID algorithm, so that the inside of the sleeve maintains a constant temperature, and the micro battery is powered by the collection control circuit;

[0007] The air intake device includes an air intake pipe and a filter screen; the air intake pipe has scale marks on the outside; the filter screen is used to isolate granular or blocky earwax from entering the air path to prevent it from being deposited in the subsequent detection system; the air intake device is connected to the sample inlet of the subsequent detection system;

[0008] The post-stage detection system is a system based on the combination of gas chromatography and surface acoustic wave sensor, including an injection port, an adsorption tube, a cluster capillary column, a surface acoustic wave sensor, a six-way valve, a carrier gas bottle, a vacuum pump, a flowmeter and a detection control circuit; port 1 of the six-way valve is connected to the injection port, ports 2 and 5 are respectively connected to two ports of the adsorption tube, port 3 is connected to the carrier gas bottle, port 4 is connected to the inlet of the cluster capillary column, port 6 is connected to the vacuum pump, and a flowmeter is arranged on the pipeline connected to the vacuum pump; the adsorption tube is filled with an adsorbent capable of concentrating, adsorbing and desorbing small molecules and macromolecules in layers; the cluster capillary column adopts a micro-nano processing technology to prepare a porous quartz column with a pore size of hundreds of microns, and a coating phase that can be used for separating gas components is modified in the pore column of the column by a dynamic film forming technology to prepare a cluster capillary column; a metal layer is wrapped around the periphery of the quartz column, and a constant voltage with an adjustable PWM duty cycle is applied to both ends of the metal, and the current change under the constant voltage is detected, which is used for heating and temperature detection of the cluster capillary column, thereby realizing controllable program heating;

[0009] The post-stage detection system includes two states: sampling state and analysis state, and the switching between the sampling state and the analysis state is realized by a six-way valve;

[0010] During the detection process, the injection port temperature was set to 80°C, the six-way valve temperature was 160°C, the cluster capillary column temperature was 40°C, the surface acoustic wave sensor temperature was 30°C, and the carrier gas flow rate was 3mL / min; after preheating, the six-way valve was switched to the sampling mode;

[0011] In the sampling mode, the six-way valve is controlled to connect ports 1 and 2, ports 3 and 4, and ports 5 and 6. The volatile gas enters the adsorption tube through the injection port for enrichment through the vacuum pump. The vacuum pump switches to the analysis mode after 30 seconds of vacuum pumping.

[0012] In the analysis mode, the six-way valve is controlled to turn, at which time ports 2 and 3 are connected, ports 4 and 5 are connected, and ports 1 and 6 are connected, the adsorption tube is instantly heated to 250°C, and the capillary column is programmed to heat up to 180°C at a rate of 10°C / s; in the sampling mode, the adsorbed components are separated inside the capillary column under the transport of inert gas as carrier gas; under programmed temperature control, the components of the mixture are gradually separated due to different moving speeds, and then flow out from the end of the capillary column in turn and deposit on the surface of the surface acoustic wave sensor, generating a frequency signal response; while the capillary column begins to heat up, the frequency signal of the surface acoustic wave sensor is recorded for 20 seconds;

[0013] After the above analysis process is completed, the surface acoustic wave sensor is heated to 120° C. and maintained for 15 seconds to fully separate the object to be measured from the sensor; a chromatogram is obtained by differentially analyzing the frequency signal of the surface acoustic wave sensor.

[0014] Furthermore, the earwax collecting device and the air intake duct are both made of volatile organic compound-free and high temperature resistant materials.

[0015] Furthermore, the sensor is a QCM mass sensitive sensor, which is composed of a quartz crystal and an electrode. When a certain mass of foreign trace matter is attached to the electrode surface of the quartz crystal, it is equivalent to an increase in the mass of the electrode. By using the piezoelectric effect of the quartz crystal, the increase in the mass of the electrode will cause a change in the corresponding frequency, and the change in mass is proportional to the change in frequency. The formula is as follows:

[0016]

[0017] Where n is the number of overtones in the thickness direction, f 0 is the fundamental frequency of the quartz crystal, S is the area of ​​the quartz surface electrode, ρ q is the density of the quartz wafer, is the piezoelectric enhanced shear modulus of the quartz wafer. When the sensor is selected, these five parameters are all constants. Then the acquisition control circuit obtains the frequency value of the sensor through the sampling module, converts it according to the formula, and obtains the mass of the collected earwax, and then communicates with the host computer through the Bluetooth module.

[0018] Furthermore, in the air intake device, the air intake pipe has scale marks on the outside, and the filter screen uses a copper screen with a mesh size greater than or equal to 60.

[0019] According to a second aspect of the present specification, a method for collecting and detecting ear canal cerumen based on the device of the first aspect is provided, the method comprising the following steps:

[0020] Earwax collection and storage: The spoon-shaped front end and the rod are pushed out of the cannula, and the cannula is used as a handle. The spoon-shaped front end is inserted into the external auditory canal of the person being collected 10-15mm to collect the secreted earwax; after the collection is completed, the spoon-shaped front end and the rod are moved into the cannula, and the cap is put on. At this time, the cannula is used as a storage container; the collection control circuit is turned on to keep the inside of the cannula at a constant temperature;

[0021] Injection testing, including:

[0022] Connect the gas inlet device and the post-stage detection system, set the injection port temperature of the post-stage detection system to 80°C, the six-way valve temperature to 160°C, the capillary column temperature to 40°C, the surface acoustic wave sensor temperature to 30°C, and the carrier gas flow to 3mL / min. After preheating, switch the six-way valve to the sampling mode;

[0023] Remove the spoon-shaped front end from the sleeve and place it into the air inlet pipe; place it for 5-10 seconds to allow the VOC substances in the earwax to volatilize, and then use a vacuum pump to evacuate for 30 seconds to allow the volatile substances to enter the adsorption tube for enrichment; switch the six-way valve to the analysis mode;

[0024] The adsorption tube is instantaneously heated to 250°C to desorb the adsorbed substances at the same time; the capillary column is programmed to heat up at a rate of 10°C / s to 180°C; the desorbed substances are separated inside the capillary column under the transport of inert gas as carrier gas, and deposited on the surface of the surface acoustic wave sensor to generate a frequency signal response; while the capillary column begins to heat up, the frequency signal of the surface acoustic wave sensor is recorded for 20 seconds;

[0025] After the above analysis process is completed, the surface acoustic wave sensor is heated to 120° C. and maintained for 15 seconds to fully separate the object to be measured from the sensor; a chromatogram is obtained by differentially analyzing the frequency signal of the surface acoustic wave sensor.

[0026] The beneficial effects of the present invention are as follows: the present invention provides an integrated device and method for the collection, storage, cold chain transportation and detection of earwax in the ear canal, providing a non-invasive, standardized collection and detection scheme for related clinical diseases. The collection of earwax avoids the disadvantage that sebum secretion samples are easily contaminated by external environmental gases, improves the accuracy of sample detection, and thus improves the accuracy of clinical disease diagnosis. The integrated design of collection and storage, and the control of the storage temperature by circuits can solve the problem of cold chain transportation in remote areas. A mass sensor is built into the spoon-shaped front end to obtain the mass of each earwax collected, thereby improving the comparability of the test results. A filter is added to the air intake device to prevent earwax particles from entering the air path and being deposited on the sensor, causing it to stop working. The detection of volatile organic compounds based on the detection system combined with gas chromatography-surface acoustic wave sensor is simple, efficient and non-invasive, and has great application value for clinical disease diagnosis. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic structural diagram of an earwax collection device provided by an exemplary embodiment;

[0028] Figure 2 is a schematic structural diagram of an air intake device provided by an exemplary embodiment;

[0029] Figure 3 It is a schematic diagram of the structure of a post-stage detection system provided by an exemplary embodiment. DETAILED DESCRIPTION

[0030] The present invention is further described in conjunction with the accompanying drawings and specific embodiments.

[0031] The embodiment of the present invention provides an earwax collection and detection device, which includes three parts: an earwax collection device, an air intake device and a post-stage detection system.

[0032] like Figure 1As shown, the earwax collection device adopts non-volatile organic compound and high temperature resistant materials, including but not limited to Teflon, ceramics, etc.; the earwax collection device includes a spoon-shaped front end 101, a sensor 102, a rod 103, a sleeve 104, a pressing plate 105, a first spring 106, a second spring 107, a third spring 108, a cap 109, a temperature control sheet 110, a micro battery 111 and a collection control circuit 112; the sensor 102 is a QCM quality sensitive sensor, which is built into the spoon-shaped front end 101, It is composed of a quartz wafer and an electrode. Its working principle is that when a certain mass of foreign trace matter adheres to the electrode surface of the quartz crystal, it is equivalent to an increase in the mass of the electrode. By using the piezoelectric effect of the quartz crystal, the increase in the mass of the electrode will cause a change in the corresponding frequency, and the change in mass is proportional to the change in frequency. Then, the acquisition control circuit obtains the frequency value of the sensor through the sampling module, converts the mass of the collected earwax according to the formula, and then communicates with the host computer through the Bluetooth module; the sleeve 104 is a hollow tube with a spoon-shaped front The end 101 is connected to the rod 103, and is pushed out of the sleeve 104 when collecting earwax, and is pushed into the sleeve 104 after the collection is completed; the pressing plate 105, the first spring 106, the second spring 107 and the third spring 108 are all internal structures at the front end of the sleeve 104, the upper ends of the first spring 106, the second spring 107 and the third spring 108 are connected to the sleeve 104, and the lower ends are connected to the pressing plate 105, and the pressing plate 105 can move up and down along the slide structure under the action of spring elastic force or external force; the spoon-shaped When the front end 101 and the rod 103 are pushed into the sleeve 104, under the elastic force of the first spring 106, the second spring 107 and the third spring 108, the pressing plate 105 will gradually flatten and compact the earwax on the spoon-shaped front end 101; the acquisition control circuit 112 uses a PID algorithm to control the temperature control plate 110 arranged inside the front end of the sleeve 104 through the temperature control module, so that the inside of the sleeve maintains a constant temperature, for example, set to 0-7 degrees Celsius, and the micro battery 111 supplies power to the acquisition control circuit 112.

[0033] like Figure 2 As shown, the air intake device includes an air intake pipe 21 and a filter screen 22; the air intake pipe 21 is made of volatile organic compound-free and high-temperature resistant materials, including but not limited to Teflon, ceramics, etc., and there are scale marks on the outside of the air intake pipe 21; the filter screen 22 is a copper mesh greater than or equal to 60 mesh, which is used to isolate granular or block earwax from entering the air path to avoid deposition in the subsequent detection system; the air intake device is connected to the sampling port of the subsequent detection system.

[0034] like Figure 3As shown, the post-stage detection system is a system based on the combination of gas chromatography and surface acoustic wave sensor, including an injection port 31, an adsorption tube 32, a cluster capillary column 33, a surface acoustic wave sensor 34, a six-way valve 35, a carrier gas bottle 36, a vacuum pump 37, a flow meter 38 and a detection control circuit 39; the port 1 of the six-way valve is connected to the injection port 31, the ports 2 and 5 are respectively connected to the two ports of the adsorption tube 32, the port 3 is connected to the carrier gas bottle 36, the port 4 is connected to the inlet of the cluster capillary column 33, the port 6 is connected to the vacuum pump 37, and a flow meter 38 is arranged on the pipeline connected to the vacuum pump 37; the adsorption tube 32 is made of 304 stainless steel tube (OD :8mm, ID: 5mm, Length: 70mm) is layered and filled with an adsorbent capable of concentrating, adsorbing and desorbing small molecules and macromolecules; the clustered capillary column 33 adopts a micro-nano processing technology to prepare a porous quartz column with a pore size of hundreds of microns, and a coating phase that can be used to separate gas components is modified in the pore column of the column by a dynamic film-forming technology to prepare the clustered capillary column 33; a metal layer is wrapped around the periphery of the quartz column, and a constant voltage with an adjustable PWM duty cycle is applied to both ends of the metal, and the current change under the constant voltage is detected, which is used for heating and temperature detection of the clustered capillary column 33, thereby realizing controllable program heating;

[0035] The post-stage detection system includes two states: sampling state and analysis state, and the switching between the sampling state and the analysis state is realized by a six-way valve 35;

[0036] During the detection process, the temperature of the injection port 31 was set to 80°C, the temperature of the six-way valve 35 was set to 160°C, the temperature of the cluster capillary column 33 was set to 40°C, the temperature of the surface acoustic wave sensor 34 was set to 30°C, and the carrier gas flow was set to 3 mL / min; after preheating, the six-way valve 35 was switched to the sampling mode;

[0037] In the sampling mode, the six-way valve 35 is controlled to connect ports 1 and 2, ports 3 and 4, and ports 5 and 6, and the volatile gas enters the adsorption tube 32 through the injection port 31 for enrichment. The vacuum pump 37 switches to the analysis mode after evacuating for 30 seconds.

[0038] In the analysis mode, the six-way valve 35 is controlled to turn, at which time, port 2 is connected to port 3, port 4 is connected to port 5, and port 1 is connected to port 6, the adsorption tube 32 is instantaneously heated to 250°C, and the capillary column 33 is programmed to heat up to 180°C at a rate of 10°C / s; in the sampling mode, the adsorbed components are separated inside the capillary column 33 under the transport of an inert gas (including but not limited to helium) as a carrier gas; under programmed temperature control, the components of the mixture are gradually separated due to different moving speeds, and then flow out from the end of the capillary column 33 in turn and deposit on the surface of the surface acoustic wave sensor 34, generating a frequency signal response; while the capillary column 33 starts to heat up, the frequency signal of the surface acoustic wave sensor 34 is recorded for 20 seconds;

[0039] After the above analysis process is completed, the surface acoustic wave sensor 34 is heated to 120° C. and maintained for 15 seconds to fully separate the object to be measured from the sensor; and a chromatogram is obtained by differentially analyzing the frequency signal of the surface acoustic wave sensor 34 .

[0040] The present invention also provides an embodiment of an ear canal cerumen collection and detection method using the above device, comprising the following steps:

[0041] Earwax collection and storage: The spoon-shaped front end 101 and the rod 103 are pushed out of the sleeve 104, and the sleeve 104 is used as a handle. The spoon-shaped front end 101 is inserted into the external auditory canal of the person being collected 10-15 mm to collect the secreted earwax; after the collection is completed, the spoon-shaped front end 101 and the rod 103 are moved into the sleeve 104, and the tube cap 109 is put on. At this time, the sleeve is used as a storage container; the collection control circuit 112 is turned on to keep the inside of the sleeve 104 at a constant temperature (0-7 degrees Celsius) so that cold chain transportation can be carried out;

[0042] Injection testing, including:

[0043] Connect the air intake device and the post-stage detection system, set the temperature of the injection port 31 of the post-stage detection system to 80°C, the temperature of the six-way valve 35 to 160°C, the temperature of the capillary column 33 to 40°C, the temperature of the surface acoustic wave sensor 34 to 30°C, and the carrier gas flow to 3 mL / min. After preheating, switch the six-way valve 35 to the sampling mode;

[0044] Remove the spoon-shaped front end 101 from the sleeve 104, and place the spoon-shaped front end 101 into the air inlet pipe 21, and the front end can be selected to be flush with the outer scale line of the air inlet pipe; place it for 5-10 seconds to allow the VOC substances in the earwax to volatilize, and then use the vacuum pump 37 to evacuate for 30 seconds to allow the volatile substances to enter the adsorption tube 32 for enrichment; the six-way valve 35 is switched to the analysis mode;

[0045] The adsorption tube 32 is instantaneously heated to 250°C, so that the adsorbed substances are desorbed at the same time; the capillary column 33 is programmed to heat up at a rate of 10°C / s to 180°C; the desorbed substances are separated inside the capillary column 33 under the transport of an inert gas (including but not limited to helium) as a carrier gas, and are deposited on the surface of the surface acoustic wave sensor 34 to generate a frequency signal response; while the capillary column 33 begins to heat up, the frequency signal of the surface acoustic wave sensor 34 is recorded for 20 seconds;

[0046] After the above analysis process is completed, the surface acoustic wave sensor 34 is heated to 120° C. and maintained for 15 seconds to fully separate the object to be measured from the sensor; and a chromatogram is obtained by differentially analyzing the frequency signal of the surface acoustic wave sensor 34 .

[0047] The above embodiments are used to illustrate the present invention rather than to limit the present invention. Any modification and change made to the present invention within the spirit of the present invention and the protection scope of the claims shall fall within the protection scope of the present invention.

Claims

1. An ear canal cerumen collection and detection device, characterized in that: It includes an earwax collection device, an air intake device and a post-stage detection system; The earwax collecting device comprises a spoon-shaped front end (101), a sensor (102), a rod (103), a sleeve (104), a pressing plate (105), a spring, a tube cap (109), a temperature control sheet (110), a micro battery (111) and a collecting control circuit (112); the sensor (102) is a QCM mass sensitive sensor, which is built into the spoon-shaped front end (101) and is used to obtain the mass of collected earwax; the sleeve (104) is a hollow tube, the spoon-shaped front end (101) and the rod (103) are connected together, and are pushed out of the sleeve (104) when collecting earwax, and are pushed into the sleeve (104) after the collection is completed; the pressing plate (105) and the spring are the sleeve (104). ) front end internal structure, the upper end of the spring is connected to the sleeve (104), and the lower end is connected to the pressing plate (105), and the pressing plate (105) can move up and down along the slide groove structure under the action of the spring force or external force; when the spoon-shaped front end (101) and the rod (103) are pushed into the sleeve (104), under the action of the spring force, the pressing plate (105) will gradually flatten and compact the earwax on the spoon-shaped front end (101); the acquisition control circuit (112) controls the temperature control sheet (110) arranged inside the front end of the sleeve (104) by adopting a PID algorithm, so that the inside of the sleeve maintains a constant temperature, and the micro battery (111) supplies power to the acquisition control circuit (112); The air intake device comprises an air intake pipe (21) and a filter (22); the air intake pipe (21) has scale marks on its exterior; the filter (22) is used to isolate granular or blocky earwax from entering the air path to prevent it from being deposited in a subsequent detection system; the air intake device is connected to a sample inlet of the subsequent detection system; The post-stage detection system is a system based on the combination of gas chromatography and surface acoustic wave sensor, comprising an injection port (31), an adsorption tube (32), a clustered capillary column (33), a surface acoustic wave sensor (34), a six-way valve (35), a carrier gas bottle (36), a vacuum pump (37), a flow meter (38) and a detection control circuit (39); port 1 of the six-way valve (35) is connected to the injection port (31), ports 2 and 5 are respectively connected to two ports of the adsorption tube (32), port 3 is connected to the carrier gas bottle (36), port 4 is connected to the inlet of the clustered capillary column (33), port 6 is connected to the vacuum pump (37), and in the tube connected to the vacuum pump (37), a flow meter (38) is connected to the detection control circuit (39). A flow meter (38) is arranged on the road; the adsorption tube (32) is filled with an adsorbent capable of concentrating, adsorbing and desorbing small molecules and large molecules in layers; the clustered capillary column (33) adopts a micro-nano processing technology to prepare a porous quartz column with a pore size of hundreds of microns, and a coating phase that can be used to separate gas components is modified in the pore column of the column by a dynamic film forming technology to prepare a clustered capillary column (33); a metal layer is wrapped around the periphery of the quartz column, and a constant voltage with an adjustable PWM duty cycle is applied to both ends of the metal, and the current change under the constant voltage is detected, which is used for heating and temperature detection of the clustered capillary column (33), thereby realizing controllable program heating; The post-stage detection system includes two states: a sampling state and an analysis state, and the switching between the sampling state and the analysis state is realized by a six-way valve (35); During the detection process, the temperature of the injection port (31) is set to 80°C, the temperature of the six-way valve (35) is set to 160°C, the temperature of the cluster capillary column (33) is set to 40°C, the temperature of the surface acoustic wave sensor (34) is set to 30°C, and the carrier gas flow rate is set to 3 mL / min; after preheating is completed, the six-way valve (35) is switched to the sampling mode; In the sampling mode, the six-way valve (35) is controlled to connect ports 1 and 2, ports 3 and 4, and ports 5 and 6, and the vacuum pump (37) is used to evacuate the gas, and the volatile gas enters the adsorption tube (32) through the injection port (31) for enrichment. The vacuum pump (37) is switched to the analysis mode after evacuating the gas for 30 seconds; In the analysis mode, the six-way valve (35) is controlled to turn, and at this time, ports 2 and 3 are connected, ports 4 and 5 are connected, and ports 1 and 6 are connected, and the adsorption tube (32) is instantly heated to 250°C, and the capillary column (33) is programmed to heat up to 180°C at a rate of 10°C / s; in the sampling mode, the adsorbed components are separated inside the capillary column (33) under the transport of inert gas as a carrier gas; under programmed temperature control, the components of the mixture are gradually separated due to different moving speeds, and then flow out from the end of the capillary column (33) in turn and deposit on the surface of the surface acoustic wave sensor (34), generating a frequency signal response; while the capillary column (33) starts to heat up, the frequency signal of the surface acoustic wave sensor (34) is recorded for 20 seconds; After the above analysis process is completed, the surface acoustic wave sensor (34) is heated to 120° C. and maintained for 15 seconds to fully separate the object to be measured from the sensor; and a chromatogram is obtained by differentially analyzing the frequency signal of the surface acoustic wave sensor (34).

2. The ear canal cerumen collection and detection device according to claim 1, characterized in that: The earwax collecting device and the air intake pipe (21) are both made of high temperature resistant materials without volatile organic compounds.

3. The ear canal cerumen collection and detection device according to claim 1, characterized in that: The sensor (102) is a QCM mass sensitive sensor, which is composed of a quartz crystal and an electrode. When a certain mass of foreign trace matter adheres to the electrode surface of the quartz crystal, it is equivalent to an increase in the mass of the electrode. By utilizing the piezoelectric effect of the quartz crystal, the increase in the mass of the electrode will cause a change in the corresponding frequency, and the change in mass is proportional to the change in frequency. The acquisition control circuit obtains the frequency value of the sensor through a sampling module, converts the mass of the collected earwax according to a formula, and then communicates with a host computer through a Bluetooth module.

4. The ear canal cerumen collection and detection device according to claim 1, characterized in that: In the air intake device, the air intake pipe (21) has scale marks on the outside, and the filter screen (22) is a copper screen with a mesh size of 60 or more.

5. A method for collecting and detecting earwax in the ear canal based on the device of claim 1, characterized in that: The method comprises the following steps: Earwax collection and storage: the spoon-shaped front end (101) and the rod (103) are pushed out of the sleeve (104), and the sleeve (104) is used as a handle. The spoon-shaped front end (101) is inserted into the external auditory canal of the person to be collected 10-15 mm to collect the secreted earwax; after the collection is completed, the spoon-shaped front end (101) and the rod (103) are moved into the sleeve (104), and the tube cap (109) is put on. At this time, the sleeve is used as a storage container; the collection control circuit (112) is turned on to keep the inside of the sleeve (104) at a constant temperature; Injection testing, including: Connect the air intake device and the post-stage detection system, set the temperature of the injection port (31) of the post-stage detection system to 80°C, the temperature of the six-way valve (35) to 160°C, the temperature of the capillary column (33) to 40°C, the temperature of the surface acoustic wave sensor (34) to 30°C, and the carrier gas flow rate to 3 mL / min. After preheating, switch the six-way valve (35) to the sampling mode; Remove the spoon-shaped front end (101) from the sleeve (104), and place the spoon-shaped front end (101) into the air inlet pipe (21); place it for 5-10 seconds to allow the VOC substances in the earwax to volatilize, and then use the vacuum pump (37) to evacuate for 30 seconds to allow the volatile substances to enter the adsorption tube (32) for enrichment; switch the six-way valve (35) to the analysis mode; The adsorption tube (32) is instantaneously heated to 250° C., so that the adsorbed substances are desorbed simultaneously; the capillary column (33) is programmed to heat up at a rate of 10° C. / s to 180° C.; the desorbed substances are separated inside the capillary column (33) under the transport of an inert gas as a carrier gas, and are deposited on the surface of a surface acoustic wave sensor (34), generating a frequency signal response; and when the capillary column (33) starts to heat up, the frequency signal of the surface acoustic wave sensor (34) is recorded for 20 seconds; After the above analysis process is completed, the surface acoustic wave sensor (34) is heated to 120° C. and maintained for 15 seconds to fully separate the object to be measured from the sensor; and a chromatogram is obtained by differentially analyzing the frequency signal of the surface acoustic wave sensor (34).

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