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Detection kit for fibrous protein or fibrinogen degradation products

A technology of fibrinogen and detection kits, applied in the direction of biological testing, measuring devices, material inspection products, etc., to achieve high sensitivity, improve detection sensitivity, and increase the effect of change intensity

Active Publication Date: 2014-12-10
上海睿康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] FDP detection methods currently used clinically include: ethanol gel test, protamine coagulation test, latex agglutination method, enzyme-linked immunosorbent assay (ELISA) method and latex immunoturbidimetric method, etc., but there are certain limitations.

Method used

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  • Detection kit for fibrous protein or fibrinogen degradation products
  • Detection kit for fibrous protein or fibrinogen degradation products
  • Detection kit for fibrous protein or fibrinogen degradation products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This embodiment relates to a detection kit for fibrin or fibrinogen degradation products, specifically as follows:

[0048] (1) R1 reagent:

[0049]

[0050] Various ingredients can be added sequentially at room temperature, sealed and stored for later use.

[0051] (2) Reagent R2:

[0052] The preparation of the latex reagent of described anti-human FDP antibody comprises the following steps:

[0053] Step 1: Activate the rabbit anti-human FDP antibody in a MOPSO solution with a pH value of 4.0-6.0;

[0054] Step 2: Adjust the pH of the solution obtained in Step 1 to 6.1 with a 5mol / L phosphate buffer solution, add carboxylated modified polystyrene latex particles with a particle size of 50-300nm to make the final concentration of the solution reach 5mg / L, and store at 37°C Incubate for 2 to 4 hours;

[0055] Step 3: Block the solution obtained in Step 2 with a blocking solution containing a phosphoric acid component of bovine serum albumin (BSA) at a p...

Embodiment 2

[0064] The FDP detection kit described in this example is applicable to various types of automatic biochemical analyzers, taking Hitachi 7170 automatic biochemical analyzer as an example, its operation is shown in Table 1. Analysis method: two-point endpoint method, that is, the dosage of R1 reagent and R2 reagent is 150ul and 50ul respectively, and the sample volume is 3ul.

[0065] Adopt this reagent and above-mentioned assay method, adopt the curve of the FDP standard substance (self-made) of 6 kinds of different contents that Hitachi 7170 biochemical analyzer records (such as figure 1 shown), each point represents a content of the reference standard, wherein the X-axis represents the FDP content (μg / ml); Y-axis represents the absorbance.

[0066] Table 1

[0067]

Embodiment 3

[0068] Example 3: Linear range

[0069] Dilute the high concentration sample (82.62μg / ml) of FDP close to the upper limit of the linear range with FDP diluent at 0.25μg / ml, 1 / 2, 1 / 10, 1 / 15, 1 / 30, 1 / 60 , be prepared into the solution of 7 different concentrations altogether, detect each concentration with the method described in embodiment 2, every concentration repeats measurement 3 times, the mean value of measured concentration and theoretical concentration are carried out linear regression analysis, and calculation regression equation is y=1.0065x +0.551, the correlation coefficient is R 2 =0.9981, indicating that the kit reagents of the present invention have good correlation in the linear range of 0.25 μg / ml~80.00 μg / ml, see for details figure 2 .

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Abstract

The invention relates to a detection kit for of fibrous protein or fibrinogen degradation products. The detection kit consists of an FDP R1 reagent, an FDP R2 reagent and an FDP standard product which are independent respectively. The invention establishes a method for determining the content of FDP in serum or blood plasma of a human body by coupling FDP antibodies and latex particles and adopting a latex-enhanced turbidimetry. Compared with the prior art, the kit can be used for detecting the FDP with the concentration range being 0.25-80microgram / ml in the serum or the blood plasma, and the problem of narrow detection linearity of the existing kit is solved; simultaneously, when being applied to carry out FDP test, the kit has the advantages of simplicity in operation, high accuracy, good repeatability and high sensitivity, and can be used on a full-automatic biochemical analyzer, a special protein instrument and a spectrophotometer.

Description

technical field [0001] The invention relates to a detection kit for fibrin or fibrinogen degradation products. Background technique [0002] The fibrinolysis system (fibrinolysis system) is the most important anticoagulant system in the human body. It maintains the normal permeability of the blood vessel wall and plays an important role in maintaining blood flow and tissue repair. The system consists of four main parts Composition: plasminogen (plasminogen), plasminogen activator (plasminogen activator, such as t-PA, u-PA), plasmin (plasmin), plasmin inhibitor (plasmin, such as PAI-1, PAI- 2, a 2AP). When a fibrin clot forms, in the presence of t-PA, plasminogen is activated and converted to plasmin, the fibrinolytic process begins, plasmin degrades fibrinogen and cross-links fibrin to form Various soluble fragments are collectively referred to as fibrin (ogen) degradation products (FDP), ie fibrin or fibrinogen degradation products (FDP). [0003] The detection of FDP co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/545
CPCG01N33/545G01N33/68
Inventor 李伟奇赵小凤房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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