Reagent kit for detecting blood serum folic acid concentration

Abstract

The invention belongs to the technical field of reagent equipment, and more particularly relates to a kit for detecting folacin concentration of blood serum (plasma), which is designed according to a detecting method based on a principle that the growth of lactobacillus casei penicillin drag-resistant strain (ATCC7469) requires folacin as essential nutrientsubstance, and prepared by the following steps that necessary reagents required by testing are matched reasonably and then assembled into the kit that is convenient for use; the solution concentration of folacin standard series is 0, 4, 8, 12, 16, 20ug/l respectively; a test sample is unnecessary to be diluted in advance, and the concentration just can be measured by sampling 5 microlitre of test sample directly; a culture medium bottle, a folacin standard liquid bottle, a lactobacillus casei liquid bottle and an antiseptic liquid bottle are arranged in the kit. The reagent is a folacin detecting method which is based on a 96-porous micro-reaction board and carries out quantitative determination by applying the turbidimetry determination of an immunization enzyme-linked determinator; and the method is low in folacin detection cost, convenient for operation and is suitable for developing folacin nutrition screening in large scale in women and children institutions.

Description

technical field [0001] The invention relates to the technical field of application of clinical in vitro diagnostic reagents, in particular to a kit for quantitatively detecting the concentration of folic acid in serum (plasma). Background technique [0002] At present, commercial kits for measuring serum (plasma) folic acid concentration are based on chemiluminescence immunoassay and radioimmunoassay, and the price is relatively high, and the determination results cannot fully reflect the biologically active folic acid components. The application in the medical field not only increases the economic burden of patients, but also makes it easy for doctors to make wrong judgments on the nutritional status of folic acid in patients. [0003] "Health Research" Volume 32, Issue 1, 56, January 2003, Huang Fuzhao; Wang Yuming; Zheng Jiaju. "Micro-determination of folic acid content in plasma and red blood cells by 96-well microbiological method" The materials used: folic acid medium,...

AI Technical Summary

Problems solved by technology

However, the existing micro-plate microbial culture method for measuring serum folic acid has a low standard solution concentration and a high medium concentration. Therefore, it is necessary to pre-dilute the serum in clinical diagnosis, and the operation is more cumbersome.

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