Method for detecting alternative biological markers of liver neoplasms in saliva, serum and urine

A technology for biomarkers and liver tumors, which is applied in the field of detection of alternative biomarkers for tumors, can solve the problems of low detection sensitivity and low accuracy of conclusions, and achieve the effects of high sensitivity, convenient specimen collection, and easy preservation

Inactive Publication Date: 2011-09-07
NORTHWEST UNIV
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Problems solved by technology

[0011] At present, the detection of relevant surrogate biomarkers is still in experimental research, the detection sensitivity and conclusion accuracy are not high

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  • Method for detecting alternative biological markers of liver neoplasms in saliva, serum and urine
  • Method for detecting alternative biological markers of liver neoplasms in saliva, serum and urine

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Embodiment Construction

[0039] Lectin is a kind of protein with sugar chain specificity that can promote cell aggregation, and it has the ability of specific, non-covalent and reversible binding to sugar chains. The lectin chip is to immobilize lectins from various sources on the glass substrate modified by formylation, epoxidation or other methods, and then react with the labeled glycoprotein, bacteria, cells and other samples to be tested. After subsequent processing, the sugar chain structure of the sample to be detected is detected. The lectin chip can detect and analyze the glycosylation modification of proteins simply, quickly and with high throughput, and provide the sugar structure fingerprint of the sample to be tested in a short time.

[0040] The present invention finds through following a large number of experimental researches:

[0041] (1) Application of CCl 4 Successfully induced mouse liver fibrosis model research found: lectin MAL-II, LTL, SBA, PSA and WGA recognized sialic acid (s...

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Abstract

The invention provides a method for detecting alternative biological markers of liver neoplasms in saliva, serum and urine so as to detect expression information of alternative biological markers of liver neoplasms in a rapid, convenient and accurate manner. The method mainly comprises the following steps of: 1) preparing agglutinin chips corresponding to detection of saliva, serum and urine samples respectively; 2) pretreating stock solutions of saliva, serum and urine samples, removing impurities and purifying proteins, labeling by using a fluorescent reagent and removing redundant fluorescence by using G-25 columns to obtain corresponding samples to be detected; and 3) respectively loading three samples to be detected onto corresponding agglutinin chips to obtain respective glycoprotein sugar chain spectrum in each sample to be detected, and obtain information of the alternative biological markers according to reaction results of any sample to be detected. By the method, the sensibility during detection is high and detection results are accurate; moreover, the method has the advantages of safety, no damage, convenience for sample collection, simplicity in storage and the like.

Description

technical field [0001] The present invention relates to a method for detecting surrogate biomarkers of tumors, in particular to a method for detecting surrogate biomarkers of liver tumors in saliva, serum and / or urine. Background technique [0002] At present, there is a lack of sensitive and specific early liver cancer diagnostic markers clinically, and the detected early liver cancer cases account for about 25% of all liver cancer cases. Although many biomacromolecules that can be used as diagnostic markers for liver cancer have been discovered, the results of these markers still remain in the diagnosis of advanced liver cancer. Alpha-fetoprotein (AFP) is currently a specific indicator for the diagnosis of liver cancer, but there are problems of false positives and false negatives in the determination of AFP. About 20% of patients with advanced liver cancer, until the death of the disease, the AFP test is still negative. However, no specific and effective molecular targe...

Claims

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Application Information

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IPC IPC(8): G01N33/96G01N33/68G01N33/533
Inventor 李铮于汉杰朱珉之秦棪楠简强张华
Owner NORTHWEST UNIV
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